Q8U1Z9 (KHSE_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	PF1054

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Homoserine kinase HAMAP MF_00384		PRO_0000156646
Regions
	Nucleotide binding	81 – 91	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8U1Z9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 293B342E084EF112	FASTA	292	31,261
	10 20 30 40 50 60 MKVYAPATIA NFGPGFDVFG LAIETPRDTV IAKESDDFRI EVEGYKVPEN DENVALVAAK 70 80 90 100 110 120 ALFKLVGEEG GIYLKLKKGI RPKSGLGSSG ASSIAGAVAA ARILGVEDDE IIIRAALEGE 130 140 150 160 170 180 RKASGSPHGD NVVPSYYGNF TIVESLNPLR VHNIEVDFKV VVILPSVEVP TSEARKVLPK 190 200 210 220 230 240 KVPLKDAVKN IALASSLVLA LKEGNLEEVG RLLEDHLALP YRLSLVPWFP KVKEAAKEAG 250 260 270 280 290 AYGVMISGSG PAVFALGENL KEIGKAMKEA FESFGIEAEY WVTKVGRGAK WC

References

[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL81178.1.
RefSeq	NP_578783.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U1Z9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000004926; EBPYRP00000004787; EBPYRG00000004925.
GeneID	1468920.
GenomeReviews	Gene locus PF1054 in contig AE009950_GR.
KEGG	pfu:PF1054.
NMPDR	fig|186497.1.peg.1091.
Phylogenomic databases
GeneTree	EBGT00050000022725.
HOGENOM	HBG646290.
OMA	GSAHADN.
PhylomeDB	Q8U1Z9.
ProtClustDB	PRK01212.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_PYRFU

Accession

Primary (citable) accession number: Q8U1Z9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program