Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8U1V2 (NADK_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:PF1103
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 277277NAD kinase HAMAP-Rule MF_00361
PRO_0000120706

Regions

Nucleotide binding67 – 682NAD By similarity
Nucleotide binding137 – 1382NAD By similarity
Nucleotide binding178 – 1836NAD By similarity

Sites

Active site671Proton acceptor By similarity
Binding site721NAD By similarity
Binding site1481NAD By similarity
Binding site1651NAD By similarity
Binding site1671NAD By similarity
Binding site2021NAD; via carbonyl oxygen By similarity
Binding site2361NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8U1V2 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 2B544411178CA954

FASTA27731,275
        10         20         30         40         50         60 
MKFGIVARRD KEEALKLAYR VYDFLKVSGY DVVVDKDTYE HFPYFNERDV IPLEEFDVDF 

        70         80         90        100        110        120 
IIAIGGDGTI LRIEHMTKKD IPILSVNMGT LGFLTEVEPS DTFFALSRLI EGEYYIDERI 

       130        140        150        160        170        180 
KVRTYINGEN RVPDALNEVA ILTGIPGKII HLKYYVDGGL ADEVRADGLV VSTPTGSTGY 

       190        200        210        220        230        240 
AMSAGGPFVD PRLDVILVVP LLPLPKTSVP MVIPGSSRVD ITLVSDREII LAIDGQYYEY 

       250        260        270 
LPPDVEITVV KSPRKTKFVR FTKEIYPKYT MKIKERH 

« Hide

References

[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009950 Genomic DNA. Translation: AAL81227.1.
RefSeqNP_578832.1. NC_003413.1.

3D structure databases

ProteinModelPortalQ8U1V2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING186497.PF1103.

Proteomic databases

PRIDEQ8U1V2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL81227; AAL81227; PF1103.
GeneID1468971.
KEGGpfu:PF1103.

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227222.
KOK00858.
OMAPRKTKFV.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_PYRFU
AccessionPrimary (citable) accession number: Q8U1V2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families