Q8U1T0 (CMR5_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CRISPR system Cmr subunit Cmr5

Alternative name(s):
CRISPR type III-B/RAMP module-associated protein Cmr5

Gene names

Name:	cmr5
Ordered Locus Names:	PF1125

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus furiosus

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage. Ref.2
Subunit structure	Part of the Cmr ribonucleoprotein complex which includes mature crRNA (39 or 45 nt in length) core components Cmr2, Cmr3, Cmr4 and Cmr6 and probably other subunits Cmr1-1, Cmr1-2 and Cmr5. Ref.2
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the CRISPR system Cmr5 family.

Ontologies

Keywords
Biological process	Antiviral defense
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 169

169

CRISPR system Cmr subunit Cmr5

PRO_0000418079

Secondary structure

1

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169

Helix Strand Turn

Details...

Helix

18 – 36

19

Helix

39 – 59

21

Helix

61 – 71

11

Helix

76 – 83

8

Beta strand

88 – 90

3

Helix

94 – 96

3

Helix

103 – 118

16

Helix

127 – 129

3

Helix

133 – 157

25

Sequences

	Sequence		Length	Mass (Da)
	Q8U1T0 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 22AF2EA37ABA2249	FASTA	169	19,687
	10 20 30 40 50 60 MEVHMLSKDN KKSIRKTLEQ RRGEYAYYVI KEVADLNDKQ LEEKYASLVK KAPVMILSNG 70 80 90 100 110 120 LLQTLAFLLA KAETSPEKAN QILSRVNEYP PRFIEKLGND KDEHLLLYLH IVYWLRENVD 130 140 150 160 RNIDVKTLLS QDYSKVLWAT KEAIALLNWM RRFAVAMLKE EGKENEGSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex." Hale C.R., Zhao P., Olson S., Duff M.O., Graveley B.R., Wells L., Terns R.M., Terns M.P. Cell 139:945-956(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION IN CMR COMPLEX, SUBCELLULAR LOCATION, SUBUNIT. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE009950 Genomic DNA. Translation: AAL81249.1.

RefSeq

NP_578854.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GKF	X-ray	2.10	A/B	1-169	[»]

ProteinModelPortal

Q8U1T0.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8U1T0. 2 interactions.

STRING

186497.PF1125.

Proteomic databases

PRIDE

Q8U1T0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL81249; AAL81249; PF1125.

GeneID

1468994.

KEGG

pfu:PF1125.

Phylogenomic databases

eggNOG

COG3337.

ProtClustDB

CLSK541883.

Family and domain databases

Gene3D

1.10.520.30. 1 hit.

InterPro

IPR023101. AF1862-like_domain.
IPR010160. CRISPR-assoc_prot_Cmr5.
[Graphical view]

Pfam

PF09701. Cas_Cmr5. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01881. cas_Cmr5. 1 hit.

ProtoNet

Search...

Entry information

Entry name

CMR5_PYRFU

Accession

Primary (citable) accession number: Q8U1T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2012
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program