Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8U1T0

- CMR5_PYRFU

UniProt

Q8U1T0 - CMR5_PYRFU

Protein

CRISPR system Cmr subunit Cmr5

Gene

cmr5

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW

    Keywords - Biological processi

    Antiviral defense

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CRISPR system Cmr subunit Cmr5
    Alternative name(s):
    CRISPR type III-B/RAMP module-associated protein Cmr5
    Gene namesi
    Name:cmr5
    Ordered Locus Names:PF1125
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 169169CRISPR system Cmr subunit Cmr5PRO_0000418079Add
    BLAST

    Proteomic databases

    PRIDEiQ8U1T0.

    Interactioni

    Subunit structurei

    Part of the Cmr ribonucleoprotein complex which includes mature crRNA (39 or 45 nt in length) core components Cmr2, Cmr3, Cmr4 and Cmr6 and probably other subunits Cmr1-1, Cmr1-2 and Cmr5.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    cmr4Q8U1S92EBI-2504964,EBI-2504950

    Protein-protein interaction databases

    DIPiDIP-54369N.
    IntActiQ8U1T0. 3 interactions.
    MINTiMINT-8408912.
    STRINGi186497.PF1125.

    Structurei

    Secondary structure

    1
    169
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi18 – 3619
    Helixi39 – 5921
    Helixi61 – 7111
    Helixi76 – 838
    Beta strandi88 – 903
    Helixi94 – 963
    Helixi103 – 11816
    Helixi127 – 1293
    Helixi133 – 15725

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GKFX-ray2.10A/B1-169[»]
    ProteinModelPortaliQ8U1T0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CRISPR system Cmr5 family.Curated

    Phylogenomic databases

    eggNOGiCOG3337.
    OMAiMLNATRI.

    Family and domain databases

    Gene3Di1.10.520.30. 1 hit.
    InterProiIPR023101. AF1862-like_domain.
    IPR010160. CRISPR-assoc_prot_Cmr5.
    [Graphical view]
    PfamiPF09701. Cas_Cmr5. 1 hit.
    [Graphical view]
    SUPFAMiSSF158568. SSF158568. 1 hit.
    TIGRFAMsiTIGR01881. cas_Cmr5. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8U1T0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVHMLSKDN KKSIRKTLEQ RRGEYAYYVI KEVADLNDKQ LEEKYASLVK    50
    KAPVMILSNG LLQTLAFLLA KAETSPEKAN QILSRVNEYP PRFIEKLGND 100
    KDEHLLLYLH IVYWLRENVD RNIDVKTLLS QDYSKVLWAT KEAIALLNWM 150
    RRFAVAMLKE EGKENEGSS 169
    Length:169
    Mass (Da):19,687
    Last modified:June 1, 2002 - v1
    Checksum:i22AF2EA37ABA2249
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81249.1.
    RefSeqiNP_578854.1. NC_003413.1.
    WP_011012265.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81249; AAL81249; PF1125.
    GeneIDi1468994.
    KEGGipfu:PF1125.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81249.1 .
    RefSeqi NP_578854.1. NC_003413.1.
    WP_011012265.1. NC_003413.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GKF X-ray 2.10 A/B 1-169 [» ]
    ProteinModelPortali Q8U1T0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-54369N.
    IntActi Q8U1T0. 3 interactions.
    MINTi MINT-8408912.
    STRINGi 186497.PF1125.

    Proteomic databases

    PRIDEi Q8U1T0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL81249 ; AAL81249 ; PF1125 .
    GeneIDi 1468994.
    KEGGi pfu:PF1125.

    Phylogenomic databases

    eggNOGi COG3337.
    OMAi MLNATRI.

    Family and domain databases

    Gene3Di 1.10.520.30. 1 hit.
    InterProi IPR023101. AF1862-like_domain.
    IPR010160. CRISPR-assoc_prot_Cmr5.
    [Graphical view ]
    Pfami PF09701. Cas_Cmr5. 1 hit.
    [Graphical view ]
    SUPFAMi SSF158568. SSF158568. 1 hit.
    TIGRFAMsi TIGR01881. cas_Cmr5. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    2. "RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex."
      Hale C.R., Zhao P., Olson S., Duff M.O., Graveley B.R., Wells L., Terns R.M., Terns M.P.
      Cell 139:945-956(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION IN CMR COMPLEX, SUBCELLULAR LOCATION, SUBUNIT.
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

    Entry informationi

    Entry nameiCMR5_PYRFU
    AccessioniPrimary (citable) accession number: Q8U1T0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2012
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3