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Q8U1T0 (CMR5_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CRISPR system Cmr subunit Cmr5
Alternative name(s):
CRISPR type III-B/RAMP module-associated protein Cmr5
Gene names
Name:cmr5
Ordered Locus Names:PF1125
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length169 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage. Ref.2

Subunit structure

Part of the Cmr ribonucleoprotein complex which includes mature crRNA (39 or 45 nt in length) core components Cmr2, Cmr3, Cmr4 and Cmr6 and probably other subunits Cmr1-1, Cmr1-2 and Cmr5. Ref.2

Subcellular location

Cytoplasm Ref.2.

Sequence similarities

Belongs to the CRISPR system Cmr5 family.

Ontologies

Keywords
   Biological processAntiviral defense
   Cellular componentCytoplasm
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to virus

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 23370277. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

cmr4Q8U1S92EBI-2504964,EBI-2504950

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 169169CRISPR system Cmr subunit Cmr5
PRO_0000418079

Secondary structure

................... 169
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8U1T0 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 22AF2EA37ABA2249

FASTA16919,687
        10         20         30         40         50         60 
MEVHMLSKDN KKSIRKTLEQ RRGEYAYYVI KEVADLNDKQ LEEKYASLVK KAPVMILSNG 

        70         80         90        100        110        120 
LLQTLAFLLA KAETSPEKAN QILSRVNEYP PRFIEKLGND KDEHLLLYLH IVYWLRENVD 

       130        140        150        160 
RNIDVKTLLS QDYSKVLWAT KEAIALLNWM RRFAVAMLKE EGKENEGSS 

« Hide

References

« Hide 'large scale' references
[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]"RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex."
Hale C.R., Zhao P., Olson S., Duff M.O., Graveley B.R., Wells L., Terns R.M., Terns M.P.
Cell 139:945-956(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION IN CMR COMPLEX, SUBCELLULAR LOCATION, SUBUNIT.
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009950 Genomic DNA. Translation: AAL81249.1.
RefSeqNP_578854.1. NC_003413.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4GKFX-ray2.10A/B1-169[»]
ProteinModelPortalQ8U1T0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-54369N.
IntActQ8U1T0. 3 interactions.
MINTMINT-8408912.
STRING186497.PF1125.

Proteomic databases

PRIDEQ8U1T0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL81249; AAL81249; PF1125.
GeneID1468994.
KEGGpfu:PF1125.

Phylogenomic databases

eggNOGCOG3337.
OMAMLNATRI.

Family and domain databases

Gene3D1.10.520.30. 1 hit.
InterProIPR023101. AF1862-like_domain.
IPR010160. CRISPR-assoc_prot_Cmr5.
[Graphical view]
PfamPF09701. Cas_Cmr5. 1 hit.
[Graphical view]
SUPFAMSSF158568. SSF158568. 1 hit.
TIGRFAMsTIGR01881. cas_Cmr5. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCMR5_PYRFU
AccessionPrimary (citable) accession number: Q8U1T0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references