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Q8U1T0

- CMR5_PYRFU

UniProt

Q8U1T0 - CMR5_PYRFU

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Protein
CRISPR system Cmr subunit Cmr5
Gene
cmr5, PF1125
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antiviral defense

Names & Taxonomyi

Protein namesi
Recommended name:
CRISPR system Cmr subunit Cmr5
Alternative name(s):
CRISPR type III-B/RAMP module-associated protein Cmr5
Gene namesi
Name:cmr5
Ordered Locus Names:PF1125
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 169169CRISPR system Cmr subunit Cmr5
PRO_0000418079Add
BLAST

Proteomic databases

PRIDEiQ8U1T0.

Interactioni

Subunit structurei

Part of the Cmr ribonucleoprotein complex which includes mature crRNA (39 or 45 nt in length) core components Cmr2, Cmr3, Cmr4 and Cmr6 and probably other subunits Cmr1-1, Cmr1-2 and Cmr5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
cmr4Q8U1S92EBI-2504964,EBI-2504950

Protein-protein interaction databases

DIPiDIP-54369N.
IntActiQ8U1T0. 3 interactions.
MINTiMINT-8408912.
STRINGi186497.PF1125.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 3619
Helixi39 – 5921
Helixi61 – 7111
Helixi76 – 838
Beta strandi88 – 903
Helixi94 – 963
Helixi103 – 11816
Helixi127 – 1293
Helixi133 – 15725

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GKFX-ray2.10A/B1-169[»]
ProteinModelPortaliQ8U1T0.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3337.
OMAiMLNATRI.

Family and domain databases

Gene3Di1.10.520.30. 1 hit.
InterProiIPR023101. AF1862-like_domain.
IPR010160. CRISPR-assoc_prot_Cmr5.
[Graphical view]
PfamiPF09701. Cas_Cmr5. 1 hit.
[Graphical view]
SUPFAMiSSF158568. SSF158568. 1 hit.
TIGRFAMsiTIGR01881. cas_Cmr5. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8U1T0-1 [UniParc]FASTAAdd to Basket

« Hide

MEVHMLSKDN KKSIRKTLEQ RRGEYAYYVI KEVADLNDKQ LEEKYASLVK    50
KAPVMILSNG LLQTLAFLLA KAETSPEKAN QILSRVNEYP PRFIEKLGND 100
KDEHLLLYLH IVYWLRENVD RNIDVKTLLS QDYSKVLWAT KEAIALLNWM 150
RRFAVAMLKE EGKENEGSS 169
Length:169
Mass (Da):19,687
Last modified:June 1, 2002 - v1
Checksum:i22AF2EA37ABA2249
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL81249.1.
RefSeqiNP_578854.1. NC_003413.1.
WP_011012265.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81249; AAL81249; PF1125.
GeneIDi1468994.
KEGGipfu:PF1125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL81249.1 .
RefSeqi NP_578854.1. NC_003413.1.
WP_011012265.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GKF X-ray 2.10 A/B 1-169 [» ]
ProteinModelPortali Q8U1T0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-54369N.
IntActi Q8U1T0. 3 interactions.
MINTi MINT-8408912.
STRINGi 186497.PF1125.

Proteomic databases

PRIDEi Q8U1T0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL81249 ; AAL81249 ; PF1125 .
GeneIDi 1468994.
KEGGi pfu:PF1125.

Phylogenomic databases

eggNOGi COG3337.
OMAi MLNATRI.

Family and domain databases

Gene3Di 1.10.520.30. 1 hit.
InterProi IPR023101. AF1862-like_domain.
IPR010160. CRISPR-assoc_prot_Cmr5.
[Graphical view ]
Pfami PF09701. Cas_Cmr5. 1 hit.
[Graphical view ]
SUPFAMi SSF158568. SSF158568. 1 hit.
TIGRFAMsi TIGR01881. cas_Cmr5. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex."
    Hale C.R., Zhao P., Olson S., Duff M.O., Graveley B.R., Wells L., Terns R.M., Terns M.P.
    Cell 139:945-956(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION IN CMR COMPLEX, SUBCELLULAR LOCATION, SUBUNIT.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiCMR5_PYRFU
AccessioniPrimary (citable) accession number: Q8U1T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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