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Protein

CRISPR-associated endoribonuclease Cas6

Gene

cas6

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), also called psiRNA (prokaryotic silencing) in this organism. This protein processes pre-crRNA into individual crRNA units, with an 8-nt 5'-repeat DNA tag that may help other proteins recognize the crRNA. Further processing occurs at their 3' termini in this organism. Generates a 5'-hydroxy and 2',3'-cyclic phosphodiester.2 Publications

Cofactori

Note: Does not require a metal cofactor.2 Publications

Temperature dependencei

Optimum temperature is 70 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei31Proton acceptor1 Publication1
Active sitei46Proton donor1 Publication1
Sitei52Transition state stabilizer1 Publication1

GO - Molecular functioni

GO - Biological processi

  • maintenance of CRISPR repeat elements Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CRISPR-associated endoribonuclease Cas6 (EC:3.1.-.-)
Gene namesi
Name:cas6
Ordered Locus Names:PF1131
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31Y → A: No RNA cleavage, binds pre-crRNA normally. 1 Publication1
Mutagenesisi46H → A: No RNA cleavage, binds pre-crRNA normally. 1 Publication1
Mutagenesisi52K → A: 40-fold reduction in RNA cleavage, binds pre-crRNA normally. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004179701 – 264CRISPR-associated endoribonuclease Cas6Add BLAST264

Interactioni

Subunit structurei

Monomer, binds precursor but not mature crRNA; is not part of the Cmr effector complex.1 Publication

Protein-protein interaction databases

STRINGi186497.PF1131.

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 14Combined sources13
Beta strandi16 – 20Combined sources5
Helixi23 – 37Combined sources15
Helixi39 – 47Combined sources9
Beta strandi53 – 56Combined sources4
Beta strandi60 – 63Combined sources4
Beta strandi71 – 86Combined sources16
Helixi88 – 100Combined sources13
Beta strandi103 – 106Combined sources4
Beta strandi109 – 118Combined sources10
Beta strandi129 – 134Combined sources6
Helixi155 – 171Combined sources17
Beta strandi172 – 175Combined sources4
Beta strandi180 – 191Combined sources12
Turni195 – 197Combined sources3
Beta strandi201 – 211Combined sources11
Helixi213 – 222Combined sources10
Beta strandi224 – 226Combined sources3
Helixi228 – 230Combined sources3
Beta strandi235 – 237Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I4HX-ray2.25X2-264[»]
3PKMX-ray3.10A/X2-264[»]
SMRiQ8U1S4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U1S4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG04342. Archaea.
COG1583. LUCA.
HOGENOMiHOG000153717.
KOiK19091.
OMAiHQYYLAS.

Family and domain databases

InterProiIPR010156. CRISPR-assoc_prot_Cas6.
[Graphical view]
PfamiPF01881. Cas_Cas6. 1 hit.
[Graphical view]
PIRSFiPIRSF005054. PF1131. 1 hit.
TIGRFAMsiTIGR01877. cas_cas6. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8U1S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLIRLVPE DKDRAFKVPY NHQYYLQGLI YNAIKSSNPK LATYLHEVKG
60 70 80 90 100
PKLFTYSLFM AEKREHPKGL PYFLGYKKGF FYFSTCVPEI AEALVNGLLM
110 120 130 140 150
NPEVRLWDER FYLHEIKVLR EPKKFNGSTF VTLSPIAVTV VRKGKSYDVP
160 170 180 190 200
PMEKEFYSII KDDLQDKYVM AYGDKPPSEF EMEVLIAKPK RFRIKPGIYQ
210 220 230 240 250
TAWHLVFRAY GNDDLLKVGY EVGFGEKNSL GFGMVKVEGN KTTKEAEEQE
260
KITFNSREEL KTGV
Length:264
Mass (Da):30,704
Last modified:June 1, 2002 - v1
Checksum:i8FB43EB6BF649D1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81255.1.
RefSeqiWP_011012271.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81255; AAL81255; PF1131.
GeneIDi1469000.
KEGGipfu:PF1131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81255.1.
RefSeqiWP_011012271.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I4HX-ray2.25X2-264[»]
3PKMX-ray3.10A/X2-264[»]
SMRiQ8U1S4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81255; AAL81255; PF1131.
GeneIDi1469000.
KEGGipfu:PF1131.

Phylogenomic databases

eggNOGiarCOG04342. Archaea.
COG1583. LUCA.
HOGENOMiHOG000153717.
KOiK19091.
OMAiHQYYLAS.

Miscellaneous databases

EvolutionaryTraceiQ8U1S4.

Family and domain databases

InterProiIPR010156. CRISPR-assoc_prot_Cas6.
[Graphical view]
PfamiPF01881. Cas_Cas6. 1 hit.
[Graphical view]
PIRSFiPIRSF005054. PF1131. 1 hit.
TIGRFAMsiTIGR01877. cas_cas6. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCAS6_PYRFU
AccessioniPrimary (citable) accession number: Q8U1S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.