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Protein

CRISPR-associated endoribonuclease Cas6

Gene

cas6

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), also called psiRNA (prokaryotic silencing) in this organism. This protein processes pre-crRNA into individual crRNA units, with an 8-nt 5'-repeat DNA tag that may help other proteins recognize the crRNA. Further processing occurs at their 3' termini in this organism. Generates a 5'-hydroxy and 2',3'-cyclic phosphodiester.2 Publications

Cofactori

Note: Does not require a metal cofactor.2 Publications

Temperature dependencei

Optimum temperature is 70 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei31 – 311Proton acceptor1 Publication
Active sitei46 – 461Proton donor1 Publication
Sitei52 – 521Transition state stabilizer1 Publication

GO - Molecular functioni

GO - Biological processi

  • maintenance of CRISPR repeat elements Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CRISPR-associated endoribonuclease Cas6 (EC:3.1.-.-)
Gene namesi
Name:cas6
Ordered Locus Names:PF1131
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi31 – 311Y → A: No RNA cleavage, binds pre-crRNA normally. 1 Publication
Mutagenesisi46 – 461H → A: No RNA cleavage, binds pre-crRNA normally. 1 Publication
Mutagenesisi52 – 521K → A: 40-fold reduction in RNA cleavage, binds pre-crRNA normally. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264CRISPR-associated endoribonuclease Cas6PRO_0000417970Add
BLAST

Proteomic databases

PRIDEiQ8U1S4.

Interactioni

Subunit structurei

Monomer, binds precursor but not mature crRNA; is not part of the Cmr effector complex.1 Publication

Protein-protein interaction databases

STRINGi186497.PF1131.

Structurei

Secondary structure

1
264
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1413Combined sources
Beta strandi16 – 205Combined sources
Helixi23 – 3715Combined sources
Helixi39 – 479Combined sources
Beta strandi53 – 564Combined sources
Beta strandi60 – 634Combined sources
Beta strandi71 – 8616Combined sources
Helixi88 – 10013Combined sources
Beta strandi103 – 1064Combined sources
Beta strandi109 – 11810Combined sources
Beta strandi129 – 1346Combined sources
Helixi155 – 17117Combined sources
Beta strandi172 – 1754Combined sources
Beta strandi180 – 19112Combined sources
Turni195 – 1973Combined sources
Beta strandi201 – 21111Combined sources
Helixi213 – 22210Combined sources
Beta strandi224 – 2263Combined sources
Helixi228 – 2303Combined sources
Beta strandi235 – 2373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I4HX-ray2.25X2-264[»]
3PKMX-ray3.10A/X2-264[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U1S4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG04342. Archaea.
COG1583. LUCA.
HOGENOMiHOG000153717.
KOiK19091.
OMAiGFFYFST.

Family and domain databases

InterProiIPR010156. CRISPR-assoc_prot_Cas6.
[Graphical view]
PfamiPF01881. Cas_Cas6. 1 hit.
[Graphical view]
PIRSFiPIRSF005054. PF1131. 1 hit.
TIGRFAMsiTIGR01877. cas_cas6. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8U1S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLIRLVPE DKDRAFKVPY NHQYYLQGLI YNAIKSSNPK LATYLHEVKG
60 70 80 90 100
PKLFTYSLFM AEKREHPKGL PYFLGYKKGF FYFSTCVPEI AEALVNGLLM
110 120 130 140 150
NPEVRLWDER FYLHEIKVLR EPKKFNGSTF VTLSPIAVTV VRKGKSYDVP
160 170 180 190 200
PMEKEFYSII KDDLQDKYVM AYGDKPPSEF EMEVLIAKPK RFRIKPGIYQ
210 220 230 240 250
TAWHLVFRAY GNDDLLKVGY EVGFGEKNSL GFGMVKVEGN KTTKEAEEQE
260
KITFNSREEL KTGV
Length:264
Mass (Da):30,704
Last modified:June 1, 2002 - v1
Checksum:i8FB43EB6BF649D1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81255.1.
RefSeqiWP_011012271.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81255; AAL81255; PF1131.
GeneIDi1469000.
KEGGipfu:PF1131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81255.1.
RefSeqiWP_011012271.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3I4HX-ray2.25X2-264[»]
3PKMX-ray3.10A/X2-264[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1131.

Proteomic databases

PRIDEiQ8U1S4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81255; AAL81255; PF1131.
GeneIDi1469000.
KEGGipfu:PF1131.

Phylogenomic databases

eggNOGiarCOG04342. Archaea.
COG1583. LUCA.
HOGENOMiHOG000153717.
KOiK19091.
OMAiGFFYFST.

Miscellaneous databases

EvolutionaryTraceiQ8U1S4.

Family and domain databases

InterProiIPR010156. CRISPR-assoc_prot_Cas6.
[Graphical view]
PfamiPF01881. Cas_Cas6. 1 hit.
[Graphical view]
PIRSFiPIRSF005054. PF1131. 1 hit.
TIGRFAMsiTIGR01877. cas_cas6. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. Cited for: FUNCTION AS AN ENDORIBONUCLEASE, RNA-BINDING, COFACTOR, ACTIVE SITE, MUTAGENESIS OF TYR-31; HIS-46 AND LYS-52.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  3. "Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes."
    Carte J., Wang R., Li H., Terns R.M., Terns M.P.
    Genes Dev. 22:3489-3496(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 2-264, FUNCTION AS AN ENDORIBONUCLEASE, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, RNA-BINDING.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  4. "Interaction of the Cas6 riboendonuclease with CRISPR RNAs: recognition and cleavage."
    Wang R., Preamplume G., Terns M.P., Terns R.M., Li H.
    Structure 19:257-264(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 2-264 IN COMPLEX WITH RNA.

Entry informationi

Entry nameiCAS6_PYRFU
AccessioniPrimary (citable) accession number: Q8U1S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.