Q8U1R8 (IF2P_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable translation initiation factor IF-2

Cleaved into the following chain:

1. Pfu infB intein
   Alternative name(s):
   Pfu IF2 intein

Gene names

Name:	infB
Ordered Locus Names:	PF1137

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	984 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 By similarity. HAMAP MF_00100_A
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP MF_00100_A
Miscellaneous	The intein interrupts the GTP-binding site. HAMAP MF_00100_A
Sequence similarities	Belongs to the IF-2 family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing Protein biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Initiation factor Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 19	19	Probable translation initiation factor IF-2, 1st part Potential		PRO_0000014490
	Chain	20 – 406	387	Pfu infB intein Potential		PRO_0000014491
	Chain	407 – 984	578	Probable translation initiation factor IF-2, 2nd part Potential		PRO_0000014492
Regions
	Domain	94 – 215	122	DOD-type homing endonuclease
	Nucleotide binding	464 – 468	5	GTP By similarity
	Nucleotide binding	518 – 521	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U1R8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: DA1277D8F666BF02	FASTA	984	110,945
	10 20 30 40 50 60 MKKIRQPIIA VLGHVDHGKC LLPEEKVVLP EIGLVTLREL FELANEVVVK DEEKEVRKLG 70 80 90 100 110 120 KMLTGVDERG NVKLLNALYV WRVAHKGEMI RVKVNGWYSV TVTPEHPFLT NRGWVKAGEL 130 140 150 160 170 180 KEGDYIAIPR RVYGNEDLMK FSKIAKELGI KGDEKEFYLA GASLDIPIKV LFLAPSKLVS 190 200 210 220 230 240 AFLRGYFDAK GVVRENYIEV PLFEDLPLLL LRFGIVSRIE KSTLKISGKR NLELFRKHVG 250 260 270 280 290 300 FTDSEKAKAL DELISKAKES ERYPILEELR RLGLLFGFTR NELRIEENPT YEVLMEILER 310 320 330 340 350 360 IERGSPNLAE KIAVLEGRIK EENYLRILEE EGLIENGKLT ELGKELLEVW RNREFDSKDV 370 380 390 400 410 420 DYVRNIVENL VFLPVEKVER IEYEGYVYDV TTETHNFVAN GILVHNTTLL DRIRKTNVAA 430 440 450 460 470 480 KEAGGITQHI GATEVPIDVV KEIAGPLIKL WKAEIKLPGL LFIDTPGHEA FTSLRARGGS 490 500 510 520 530 540 LADLAVLVVD INEGFQPQTI ESIEILRRYK TPFVVAANKI DRIKGWVIQE DEPFLLNSKR 550 560 570 580 590 600 QDQRAIQELE TKLWELIGKF YEFGFQANRF DRVQNFTREL AIVPISAKYG IGIAELLVLI 610 620 630 640 650 660 AGLSQKYLEE RLKIEVEGPA RGTILEVREE PGLGHTIDVI IYEGTLHKDD TIVVGGKDKA 670 680 690 700 710 720 IVTKVRALLK PKPLDEIRDP RFRFDYVDEV TAAAGVKIAA PGLEEALAGS PVIAAPTPEA 730 740 750 760 770 780 VEKAKEEIMR QIQSVVISTD KMGVIIKADT LGSLEALSKE LQEKGIPIRK ADVGNISKTD 790 800 810 820 830 840 VMEALSVREE DPKYGVIIGF NVKVNEDAQE IAKAKGVPIF VGNIIYKLIE DYEAWVKEEE 850 860 870 880 890 900 EKKKRELLAK VTFPGVIRLY PDERYVFRRS NPAIVGIEVI EGRIKPGVTL IKQNGQKVGT 910 920 930 940 950 960 IKSIKSRDEF LQEAKKGQAV AVAIEGAIVG RHIHPGETLY VDISRDDAIT LLKYLRDVLE 970 980 DSDIKALKII AQIKAKEDPF WRAI

References

[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL81261.1.
RefSeq	NP_578866.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U1R8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000004080; EBPYRP00000003941; EBPYRG00000004080.
GeneID	1469006.
GenomeReviews	Gene locus PF1137 in contig AE009950_GR.
KEGG	pfu:PF1137.
NMPDR	fig|186497.1.peg.1177.
Phylogenomic databases
GeneTree	EBGT00050000022153.
HOGENOM	HBG645564.
OMA	ELLEVWR.
PhylomeDB	Q8U1R8.
ProtClustDB	PRK14845.
Family and domain databases
HAMAP	MF_00100_A. IF-2_A. [Tree]
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR000795. ProtSyn_GTP-bd. IPR005225. Small_GTP-bd_dom. IPR004544. TIF_aIF-2_arc. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.40.50.10050. TIF_IF2_dom3. 1 hit.
KO	K03243.
PANTHER	PTHR23115:SF41. aIF-2. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00491. AIF-2. 1 hit. TIGR01443. Intein_Cterm. 1 hit. TIGR01445. Intein_Nterm. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS01176. IF2. False negative. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2P_PYRFU

Accession

Primary (citable) accession number: Q8U1R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program