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Protein

NAD-dependent protein deacylase

Gene

cobB

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.UniRule annotation

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.UniRule annotation
NAD+ + a succinylprotein = nicotinamide + O-succinyl-ADP-ribose + a protein.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641SubstrateUniRule annotation
Binding sitei67 – 671SubstrateUniRule annotation
Active sitei116 – 1161Proton acceptorUniRule annotation
Metal bindingi124 – 1241ZincUniRule annotation
Metal bindingi127 – 1271ZincUniRule annotation
Metal bindingi150 – 1501ZincUniRule annotation
Metal bindingi153 – 1531ZincUniRule annotation
Binding sitei234 – 2341NAD; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 3920NADUniRule annotationAdd
BLAST
Nucleotide bindingi98 – 1014NADUniRule annotation
Nucleotide bindingi190 – 1923NADUniRule annotation
Nucleotide bindingi216 – 2183NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacylaseUniRule annotation (EC:3.5.1.-UniRule annotation)
Alternative name(s):
Regulatory protein SIR2 homologUniRule annotation
Gene namesi
Name:cobBUniRule annotation
Ordered Locus Names:PF1154
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250NAD-dependent protein deacylasePRO_0000110384Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi186497.PF1154.

Structurei

3D structure databases

ProteinModelPortaliQ8U1Q1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 250248Deacetylase sirtuin-typeUniRule annotationAdd
BLAST

Domaini

2 residues (Tyr-64 and Arg-67) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.UniRule annotation

Sequence similaritiesi

Belongs to the sirtuin family. Class III subfamily.UniRule annotation
Contains 1 deacetylase sirtuin-type domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04248. Archaea.
COG0846. LUCA.
HOGENOMiHOG000085950.
KOiK12410.
OMAiLIHMHGE.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01121. Sirtuin_ClassIII.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR027546. Sirtuin_class_III.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U1Q1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGEVSKILA KSSMAIAFTG AGISAESGIP TFRGKDGLWR KYRAEELATP
60 70 80 90 100
EAFKRDPKLV WEFYKWRIKK ILEAKPNPAH IALAELEKMG IIKAVITQNV
110 120 130 140 150
DDLHREAGSK NVIELHGNIF RVKCTSCSYR EYLKESDRIG WLLSQELPRC
160 170 180 190 200
PKCGSLLRPD VVWFGEALPE KELTTAFSLA KKADVVLVVG TSGVVYPAAY
210 220 230 240 250
IPYIVKESGG IVVEINIEPS AITPIADFFL RGKAGEVLPK LVEEIRRISK
Length:250
Mass (Da):27,786
Last modified:October 31, 2003 - v2
Checksum:i8028E229C286E8CC
GO

Sequence cautioni

The sequence AAL81278.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81278.1. Different initiation.
RefSeqiWP_011012294.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81278; AAL81278; PF1154.
GeneIDi1469023.
KEGGipfu:PF1154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81278.1. Different initiation.
RefSeqiWP_011012294.1. NC_003413.1.

3D structure databases

ProteinModelPortaliQ8U1Q1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81278; AAL81278; PF1154.
GeneIDi1469023.
KEGGipfu:PF1154.

Phylogenomic databases

eggNOGiarCOG04248. Archaea.
COG0846. LUCA.
HOGENOMiHOG000085950.
KOiK12410.
OMAiLIHMHGE.

Family and domain databases

Gene3Di3.30.1600.10. 2 hits.
3.40.50.1220. 3 hits.
HAMAPiMF_01121. Sirtuin_ClassIII.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR027546. Sirtuin_class_III.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiNPD_PYRFU
AccessioniPrimary (citable) accession number: Q8U1Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: July 6, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.