Putative chitinase - Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Preferred order of sections

Names and origin

Protein names

Submitted name:
Putative chitinase EMBL AAL81357.1

Gene names

Ordered Locus Names:

PF1233

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	717 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 3A4X PDB 2CZN PDB 2CWR PDB 2DSK PDB 3A4W PDB 3AFB Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

587 – 590

4

N-acetyl-D-glucosamine binding PDB 3A4X

Sites

Binding site

421

1

N-acetyl-D-glucosamine PDB 3A4X

Binding site

490

1

N-acetyl-D-glucosamine; via amide nitrogen PDB 3A4X

Binding site

526

1

N-acetyl-D-glucosamine PDB 3A4X

Binding site

555

1

N-acetyl-D-glucosamine PDB 3A4X

Binding site

636

1

N-acetyl-D-glucosamine PDB 3A4X

Binding site

664

1

N-acetyl-D-glucosamine PDB 3A4X

Binding site

679

1

N-acetyl-D-glucosamine PDB 3A4X

Sequences

Sequence

Length

Mass (Da)

Tools

Q8U1H5 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: FBCB55B9C850E38B
Show »

FASTA

717

78,635

        10         20         30         40         50         60 
MKYLDFINVM TYDYHGAWDP ITGHLAPLYA DPNAPYEDPN IKWNFNVNAS IQWYLKHGVN 

        70         80         90        100        110        120 
PKQLGLGLPF YGRSFANVPP ENNGLYQPFS GTPAGTWGPA YETHGVMDYW DIEEKRASGQ 

       130        140        150        160        170        180 
YNYYWDPVAM VPWLYSPSLK IFISYDDKKS IGIKVDYALK YNLGGVMVWE ITADRKPGTN 

       190        200        210        220        230        240 
SHPLLDTILE HIAQGGGVVP TPSPTPTPTP TPTPTPTTTT TTSTPTPTTT TTPSPTTTTT 

       250        260        270        280        290        300 
TTTTTTTTST TTTPTTTTTP VPVSGSLEVK VNDWGSGAEY DVTLNLDGQY DWTVKVKLAP 

       310        320        330        340        350        360 
GATVGSFWSA NKQEGNGYVI FTPVSWNKGP TATFGFIVNG PQGDKVEEIT LEINGQVIDI 

       370        380        390        400        410        420 
WTPTGGTTPT PTTTTTSTPT PSQTPTPTPT PTPTPTPTPT LTPTPLPGNA NPIPEHFFAP 

       430        440        450        460        470        480 
YIDMSLSVHK PLVEYAKLTG TKYFTLAFIL YSSVYNGPAW AGSIPLEKFV DEVRELREIG 

       490        500        510        520        530        540 
GEVIIAFGGA VGPYLCQQAS TPEQLAEWYI KVIDTYNATY LDFDIEAGID ADKLADALLI 

       550        560        570        580        590        600 
VQRERPWVKF SFTLPSDPGI GLAGGYGIIE TMAKKGVRVD RVNPMTMDYY WTPSNAENAI 

       610        620        630        640        650        660 
KVAENVFRQL KQIYPEKSDE EIWKMIGLTP MIGVNDDKSV FTLEDAQQLV DWAIQHKIGS 

       670        680        690        700        710 
LAFWSVDRDH PGPTGEVSPL HRGTNDPDWA FSHVFVKFME AFGYTFSAQT SEASVPT

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tertiary structure and carbohydrate recognition by the chitin-binding domain of a hyperthermophilic chitinase from Pyrococcus furiosus." Nakamura T., Mine S., Hagihara Y., Ishikawa K., Ikegami T., Uegaki K. J. Mol. Biol. 381:670-680(2008) [PubMed: 18582475] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 258-358.
[2]	"Structure of the catalytic domain of the hyperthermophilic chitinase from Pyrococcus furiosus." Nakamura T., Mine S., Hagihara Y., Ishikawa K., Uegaki K. Acta Crystallogr. F 63:7-11(2007) [PubMed: 17183162] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 409-717.
[3]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[4]	"Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus- the role of conserved residues in the active site." Tsuji H., Nishimura S., Inui T., Kado Y., Ishikawa K., Nakamura T., Uegaki K. FEBS J. 277:2683-2695(2010) [PubMed: 20553502] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 409-717 IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE009950 Genomic DNA. Translation: AAL81357.1.

RefSeq

NP_578962.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CWR	X-ray	1.70	A	258-358	[»]
2CZN	NMR	-	A	258-358	[»]
2DSK	X-ray	1.50	A/B	409-717	[»]
3A4W	X-ray	1.80	A/B	409-717	[»]
3A4X	X-ray	1.76	A/B	409-717	[»]
3AFB	X-ray	1.76	A/B	409-717	[»]

ProteinModelPortal

Q8U1H5.

SMR

Q8U1H5. Positions 258-358, 409-706.

ModBase

Search...

Protein family/group databases

CAZy

CBM2. Carbohydrate-Binding Module Family 2.
GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBPYRT00000004871; EBPYRP00000004732; EBPYRG00000004870.

GeneID

1469105.

GenomeReviews

Gene locus PF1233 in contig AE009950_GR.

KEGG

pfu:PF1233.

NMPDR

fig|186497.1.peg.1276.

Phylogenomic databases

GeneTree

EBGT00050000023457.

ProtClustDB

CLSK491906.

Family and domain databases

InterPro

IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR001223. Glyco_hydro18cat.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:2.60.40.290. CBD_carb_bd. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 3 hits.

KO

K01183.

Pfam

PF00553. CBM_2. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]

SMART

SM00637. CBD_II. 1 hit.
[Graphical view]

SUPFAM

SSF49384. Cellul_bind. 1 hit.
SSF51445. Glyco_hydro_cat. 2 hits.

PROSITE

PS51173. CBM2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q8U1H5_PYRFU

Accession

Primary (citable) accession number: Q8U1H5

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)