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Q8U191 (THIM_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:PF1335
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000156971

Sites

Binding site431Substrate; via amide nitrogen By similarity
Binding site1181ATP By similarity
Binding site1651ATP By similarity
Binding site1921Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8U191 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 43994B6A6CFAAD20

FASTA26528,327
        10         20         30         40         50         60 
MISVGEALAK VRERKPLVHN ITNFVVMNTT ANALLALGAS PVMAHAEEEL EEMVRIADSV 

        70         80         90        100        110        120 
VVNIGTLDKF WINSMIKAAK LAKEYGKPLV LDPVGAGATR LRTKTALEVL KIGATIVKGN 

       130        140        150        160        170        180 
FGEISALLGE EGKTRGVDSS TYDPERAKEL ALAVAEEFST IVAVTGKVDY VSDGKRVYAV 

       190        200        210        220        230        240 
YNGHELLGRV TGTGCIVAAL TGAFVAVNDP LTSAVSSLVV FEVAAEKAAE EAKAPGTFHA 

       250        260 
KLYDWLYLLT PEDVERLKKI EVVKV 

« Hide

References

[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009950 Genomic DNA. Translation: AAL81459.1.
RefSeqNP_579064.1. NC_003413.1.

3D structure databases

ProteinModelPortalQ8U191.
SMRQ8U191. Positions 6-264.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING186497.PF1335.

Proteomic databases

PRIDEQ8U191.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL81459; AAL81459; PF1335.
GeneID1469210.
KEGGpfu:PF1335.

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAVSGTGCM.

Enzyme and pathway databases

UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_PYRFU
AccessionPrimary (citable) accession number: Q8U191
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways