SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8U160

- RL7A_PYRFU

UniProt

Q8U160 - RL7A_PYRFU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
50S ribosomal protein L7Ae
Gene
rpl7ae, PF1367
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs. Component of the 70S ribosome. Component of a box C/D small ribonucleoprotein (sRNP) particle that is involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.UniRule annotation

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-KW
  2. ribonuclease P activity Source: UniProtKB-HAMAP
  3. structural constituent of ribosome Source: InterPro

GO - Biological processi

  1. rRNA processing Source: UniProtKB-KW
  2. tRNA 5'-leader removal Source: UniProtKB-HAMAP
  3. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L7Ae
Alternative name(s):
Ribosomal protein L8e
Gene namesi
Name:rpl7ae
Ordered Locus Names:PF1367
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. ribosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12312350S ribosomal protein L7AeUniRule annotation
PRO_0000136802Add
BLAST

Proteomic databases

PRIDEiQ8U160.

Interactioni

Subunit structurei

Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme. Probably part of the RNase P complex.1 Publication

Protein-protein interaction databases

DIPiDIP-48528N.
STRINGi186497.PF1367.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2917
Beta strandi30 – 356
Helixi36 – 449
Beta strandi49 – 557
Helixi60 – 623
Turni63 – 653
Helixi66 – 738
Beta strandi77 – 815
Helixi83 – 897
Beta strandi92 – 943
Beta strandi97 – 1037
Helixi105 – 1073
Helixi108 – 12215

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HVYX-ray2.30D1-123[»]
3HAXX-ray2.11D2-123[»]
3HAYX-ray4.99D2-123[»]
3HJWX-ray2.35C3-122[»]
3J20electron microscopy6.6031-123[»]
3J21electron microscopy6.604/G1-123[»]
3J43electron microscopy9.0031-123[»]
3J44electron microscopy9.00G1-123[»]
3LWOX-ray2.86C1-123[»]
3LWPX-ray2.50C1-123[»]
3LWQX-ray2.68C1-123[»]
3LWRX-ray2.20C1-123[»]
3LWVX-ray2.50C1-123[»]
3NMUX-ray2.73C/G2-123[»]
3NVIX-ray2.71B/D2-123[»]
3NVKX-ray3.21E/H2-123[»]
4BY9NMR-D/G/J/M3-123[»]
ProteinModelPortaliQ8U160.
SMRiQ8U160. Positions 3-123.

Miscellaneous databases

EvolutionaryTraceiQ8U160.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1358.
HOGENOMiHOG000055225.
KOiK02936.
OMAiYANSATF.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
HAMAPiMF_00326. Ribosomal_L7Ae_Arch.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.
SUPFAMiSSF55315. SSF55315. 1 hit.
TIGRFAMsiTIGR03677. rpl7ae. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U160-1 [UniParc]FASTAAdd to Basket

« Hide

MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL    50
VIIAEDVDPE EIVAHLPPLC EEKEIPYIYV PSKKELGAAA GIEVAAASVA 100
IIEPGKARDL VEEIAMKVKE LMK 123
Length:123
Mass (Da):13,395
Last modified:August 30, 2005 - v2
Checksum:i934817F41BE2D4C5
GO

Sequence cautioni

The sequence AAL81491.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL81491.1. Different initiation.
RefSeqiNP_579096.1. NC_003413.1.
WP_011012514.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81491; AAL81491; PF1367.
GeneIDi1469243.
KEGGipfu:PF1367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL81491.1 . Different initiation.
RefSeqi NP_579096.1. NC_003413.1.
WP_011012514.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HVY X-ray 2.30 D 1-123 [» ]
3HAX X-ray 2.11 D 2-123 [» ]
3HAY X-ray 4.99 D 2-123 [» ]
3HJW X-ray 2.35 C 3-122 [» ]
3J20 electron microscopy 6.60 3 1-123 [» ]
3J21 electron microscopy 6.60 4/G 1-123 [» ]
3J43 electron microscopy 9.00 3 1-123 [» ]
3J44 electron microscopy 9.00 G 1-123 [» ]
3LWO X-ray 2.86 C 1-123 [» ]
3LWP X-ray 2.50 C 1-123 [» ]
3LWQ X-ray 2.68 C 1-123 [» ]
3LWR X-ray 2.20 C 1-123 [» ]
3LWV X-ray 2.50 C 1-123 [» ]
3NMU X-ray 2.73 C/G 2-123 [» ]
3NVI X-ray 2.71 B/D 2-123 [» ]
3NVK X-ray 3.21 E/H 2-123 [» ]
4BY9 NMR - D/G/J/M 3-123 [» ]
ProteinModelPortali Q8U160.
SMRi Q8U160. Positions 3-123.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48528N.
STRINGi 186497.PF1367.

Proteomic databases

PRIDEi Q8U160.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL81491 ; AAL81491 ; PF1367 .
GeneIDi 1469243.
KEGGi pfu:PF1367.

Phylogenomic databases

eggNOGi COG1358.
HOGENOMi HOG000055225.
KOi K02936.
OMAi YANSATF.

Miscellaneous databases

EvolutionaryTracei Q8U160.

Family and domain databases

Gene3Di 3.30.1330.30. 1 hit.
HAMAPi MF_00326. Ribosomal_L7Ae_Arch.
InterProi IPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view ]
Pfami PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view ]
PRINTSi PR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.
SUPFAMi SSF55315. SSF55315. 1 hit.
TIGRFAMsi TIGR03677. rpl7ae. 1 hit.
PROSITEi PS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle."
    Xue S., Wang R., Yang F., Terns R.M., Terns M.P., Zhang X., Maxwell E.S., Li H.
    Mol. Cell 39:939-949(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.71 ANGSTROMS) IN COMPLEXES WITH FLPA NOP5 AND BOX C/D RNA, SUBUNIT.
  3. "Promiscuous behaviour of archaeal ribosomal proteins: implications for eukaryotic ribosome evolution."
    Armache J.P., Anger A.M., Marquez V., Franckenberg S., Frohlich T., Villa E., Berninghausen O., Thomm M., Arnold G.J., Beckmann R., Wilson D.N.
    Nucleic Acids Res. 41:1284-1293(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (6.6 ANGSTROMS) OF 70S RIBOSOME.

Entry informationi

Entry nameiRL7A_PYRFU
AccessioniPrimary (citable) accession number: Q8U160
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: August 30, 2005
Last modified: September 3, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi