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Protein

50S ribosomal protein L7Ae

Gene

rpl7ae

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs. Component of the 70S ribosome. Component of a box C/D small ribonucleoprotein (sRNP) particle that is involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L7AeUniRule annotation
Alternative name(s):
Ribosomal protein L8eUniRule annotation
Gene namesi
Name:rpl7aeUniRule annotation
Ordered Locus Names:PF1367
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12312350S ribosomal protein L7AePRO_0000136802Add
BLAST

Proteomic databases

PRIDEiQ8U160.

Interactioni

Subunit structurei

Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme. Probably part of the RNase P complex.1 Publication

Protein-protein interaction databases

DIPiDIP-48528N.
STRINGi186497.PF1367.

Structurei

Secondary structure

1
123
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2917Combined sources
Beta strandi30 – 356Combined sources
Helixi36 – 449Combined sources
Beta strandi49 – 557Combined sources
Helixi60 – 623Combined sources
Turni63 – 653Combined sources
Helixi66 – 738Combined sources
Beta strandi77 – 815Combined sources
Helixi83 – 897Combined sources
Beta strandi92 – 943Combined sources
Beta strandi97 – 1037Combined sources
Helixi105 – 1073Combined sources
Helixi108 – 12215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HVYX-ray2.30D1-123[»]
3HAXX-ray2.11D2-123[»]
3HAYX-ray4.99D2-123[»]
3HJWX-ray2.35C3-122[»]
3LWOX-ray2.86C1-123[»]
3LWPX-ray2.50C1-123[»]
3LWQX-ray2.68C1-123[»]
3LWRX-ray2.20C1-123[»]
3LWVX-ray2.50C1-123[»]
3NMUX-ray2.73C/G2-123[»]
3NVIX-ray2.71B/D2-123[»]
3NVKX-ray3.21E/H2-123[»]
4BY9NMR-D/G/J/M3-123[»]
4V4Nelectron microscopy9.003/G1-123[»]
4V6Uelectron microscopy6.60A3/B4/BG1-123[»]
ProteinModelPortaliQ8U160.
SMRiQ8U160. Positions 3-123.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U160.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L7Ae family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1358.
HOGENOMiHOG000055225.
KOiK02936.
OMAiMAKAIYV.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
HAMAPiMF_00326. Ribosomal_L7Ae_Arch.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.
SUPFAMiSSF55315. SSF55315. 1 hit.
TIGRFAMsiTIGR03677. eL8_ribo. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U160-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL
60 70 80 90 100
VIIAEDVDPE EIVAHLPPLC EEKEIPYIYV PSKKELGAAA GIEVAAASVA
110 120
IIEPGKARDL VEEIAMKVKE LMK
Length:123
Mass (Da):13,395
Last modified:August 30, 2005 - v2
Checksum:i934817F41BE2D4C5
GO

Sequence cautioni

The sequence AAL81491.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81491.1. Different initiation.
RefSeqiNP_579096.1. NC_003413.1.
WP_011012514.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81491; AAL81491; PF1367.
GeneIDi1469243.
KEGGipfu:PF1367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81491.1. Different initiation.
RefSeqiNP_579096.1. NC_003413.1.
WP_011012514.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HVYX-ray2.30D1-123[»]
3HAXX-ray2.11D2-123[»]
3HAYX-ray4.99D2-123[»]
3HJWX-ray2.35C3-122[»]
3LWOX-ray2.86C1-123[»]
3LWPX-ray2.50C1-123[»]
3LWQX-ray2.68C1-123[»]
3LWRX-ray2.20C1-123[»]
3LWVX-ray2.50C1-123[»]
3NMUX-ray2.73C/G2-123[»]
3NVIX-ray2.71B/D2-123[»]
3NVKX-ray3.21E/H2-123[»]
4BY9NMR-D/G/J/M3-123[»]
4V4Nelectron microscopy9.003/G1-123[»]
4V6Uelectron microscopy6.60A3/B4/BG1-123[»]
ProteinModelPortaliQ8U160.
SMRiQ8U160. Positions 3-123.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48528N.
STRINGi186497.PF1367.

Proteomic databases

PRIDEiQ8U160.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81491; AAL81491; PF1367.
GeneIDi1469243.
KEGGipfu:PF1367.

Phylogenomic databases

eggNOGiCOG1358.
HOGENOMiHOG000055225.
KOiK02936.
OMAiMAKAIYV.

Miscellaneous databases

EvolutionaryTraceiQ8U160.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
HAMAPiMF_00326. Ribosomal_L7Ae_Arch.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.
SUPFAMiSSF55315. SSF55315. 1 hit.
TIGRFAMsiTIGR03677. eL8_ribo. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle."
    Xue S., Wang R., Yang F., Terns R.M., Terns M.P., Zhang X., Maxwell E.S., Li H.
    Mol. Cell 39:939-949(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.71 ANGSTROMS) IN COMPLEXES WITH FLPA NOP5 AND BOX C/D RNA, SUBUNIT.
  3. "Promiscuous behaviour of archaeal ribosomal proteins: implications for eukaryotic ribosome evolution."
    Armache J.P., Anger A.M., Marquez V., Franckenberg S., Frohlich T., Villa E., Berninghausen O., Thomm M., Arnold G.J., Beckmann R., Wilson D.N.
    Nucleic Acids Res. 41:1284-1293(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (6.6 ANGSTROMS) OF 70S RIBOSOME.

Entry informationi

Entry nameiRL7A_PYRFU
AccessioniPrimary (citable) accession number: Q8U160
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: August 30, 2005
Last modified: April 29, 2015
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.