Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8U160 (RL7A_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L7Ae
Alternative name(s):
Ribosomal protein L8e
Gene names
Name:rpl7ae
Ordered Locus Names:PF1367
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length123 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs. Component of the 70S ribosome. Component of a box C/D small ribonucleoprotein (sRNP) particle that is involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. HAMAP-Rule MF_00326

Subunit structure

Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme. Probably part of the RNase P complex. Ref.2

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00326.

Sequence similarities

Belongs to the ribosomal protein L7Ae family.

Sequence caution

The sequence AAL81491.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processrRNA processing
tRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
rRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processrRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA 5'-leader removal

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionrRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonuclease P activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

structural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12312350S ribosomal protein L7Ae HAMAP-Rule MF_00326
PRO_0000136802

Secondary structure

...................... 123
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8U160 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: 934817F41BE2D4C5

FASTA12313,395
        10         20         30         40         50         60 
MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL VIIAEDVDPE 

        70         80         90        100        110        120 
EIVAHLPPLC EEKEIPYIYV PSKKELGAAA GIEVAAASVA IIEPGKARDL VEEIAMKVKE 


LMK 

« Hide

References

« Hide 'large scale' references
[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]"Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle."
Xue S., Wang R., Yang F., Terns R.M., Terns M.P., Zhang X., Maxwell E.S., Li H.
Mol. Cell 39:939-949(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.71 ANGSTROMS) IN COMPLEXES WITH FLPA NOP5 AND BOX C/D RNA, SUBUNIT.
[3]"Promiscuous behaviour of archaeal ribosomal proteins: implications for eukaryotic ribosome evolution."
Armache J.P., Anger A.M., Marquez V., Franckenberg S., Frohlich T., Villa E., Berninghausen O., Thomm M., Arnold G.J., Beckmann R., Wilson D.N.
Nucleic Acids Res. 41:1284-1293(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (6.6 ANGSTROMS) OF 70S RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009950 Genomic DNA. Translation: AAL81491.1. Different initiation.
RefSeqNP_579096.1. NC_003413.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2HVYX-ray2.30D1-123[»]
3HAXX-ray2.11D2-123[»]
3HAYX-ray4.99D2-123[»]
3HJWX-ray2.35C3-122[»]
3J20electron microscopy6.6031-123[»]
3J21electron microscopy6.604/G1-123[»]
3J43electron microscopy9.0031-123[»]
3J44electron microscopy9.00G1-123[»]
3LWOX-ray2.86C1-123[»]
3LWPX-ray2.50C1-123[»]
3LWQX-ray2.68C1-123[»]
3LWRX-ray2.20C1-123[»]
3LWVX-ray2.50C1-123[»]
3NMUX-ray2.73C/G2-123[»]
3NVIX-ray2.71B/D2-123[»]
3NVKX-ray3.21E/H2-123[»]
4BY9NMR-D/G/J/M3-123[»]
ProteinModelPortalQ8U160.
SMRQ8U160. Positions 3-123.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48528N.
STRING186497.PF1367.

Proteomic databases

PRIDEQ8U160.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL81491; AAL81491; PF1367.
GeneID1469243.
KEGGpfu:PF1367.

Phylogenomic databases

eggNOGCOG1358.
HOGENOMHOG000055225.
KOK02936.
OMAYANSATF.

Family and domain databases

Gene3D3.30.1330.30. 1 hit.
HAMAPMF_00326. Ribosomal_L7Ae_Arch.
InterProIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view]
PfamPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSPR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.
SUPFAMSSF55315. SSF55315. 1 hit.
TIGRFAMsTIGR03677. rpl7ae. 1 hit.
PROSITEPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8U160.

Entry information

Entry nameRL7A_PYRFU
AccessionPrimary (citable) accession number: Q8U160
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: August 30, 2005
Last modified: July 9, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references