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Q8U160

- RL7A_PYRFU

UniProt

Q8U160 - RL7A_PYRFU

Protein

50S ribosomal protein L7Ae

Gene

rpl7ae

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs. Component of the 70S ribosome. Component of a box C/D small ribonucleoprotein (sRNP) particle that is involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.

    GO - Molecular functioni

    1. ribonuclease P activity Source: UniProtKB-HAMAP
    2. rRNA binding Source: UniProtKB-KW
    3. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. rRNA processing Source: UniProtKB-KW
    2. translation Source: UniProtKB-HAMAP
    3. tRNA 5'-leader removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    rRNA processing, tRNA processing

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L7AeUniRule annotation
    Alternative name(s):
    Ribosomal protein L8eUniRule annotation
    Gene namesi
    Name:rpl7aeUniRule annotation
    Ordered Locus Names:PF1367
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. ribosome Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12312350S ribosomal protein L7AePRO_0000136802Add
    BLAST

    Proteomic databases

    PRIDEiQ8U160.

    Interactioni

    Subunit structurei

    Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme. Probably part of the RNase P complex.1 Publication

    Protein-protein interaction databases

    DIPiDIP-48528N.
    STRINGi186497.PF1367.

    Structurei

    Secondary structure

    1
    123
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi13 – 2917
    Beta strandi30 – 356
    Helixi36 – 449
    Beta strandi49 – 557
    Helixi60 – 623
    Turni63 – 653
    Helixi66 – 738
    Beta strandi77 – 815
    Helixi83 – 897
    Beta strandi92 – 943
    Beta strandi97 – 1037
    Helixi105 – 1073
    Helixi108 – 12215

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HVYX-ray2.30D1-123[»]
    3HAXX-ray2.11D2-123[»]
    3HAYX-ray4.99D2-123[»]
    3HJWX-ray2.35C3-122[»]
    3J20electron microscopy6.6031-123[»]
    3J21electron microscopy6.604/G1-123[»]
    3J43electron microscopy9.0031-123[»]
    3J44electron microscopy9.00G1-123[»]
    3LWOX-ray2.86C1-123[»]
    3LWPX-ray2.50C1-123[»]
    3LWQX-ray2.68C1-123[»]
    3LWRX-ray2.20C1-123[»]
    3LWVX-ray2.50C1-123[»]
    3NMUX-ray2.73C/G2-123[»]
    3NVIX-ray2.71B/D2-123[»]
    3NVKX-ray3.21E/H2-123[»]
    4BY9NMR-D/G/J/M3-123[»]
    ProteinModelPortaliQ8U160.
    SMRiQ8U160. Positions 3-123.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8U160.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L7Ae family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1358.
    HOGENOMiHOG000055225.
    KOiK02936.
    OMAiYANSATF.

    Family and domain databases

    Gene3Di3.30.1330.30. 1 hit.
    HAMAPiMF_00326. Ribosomal_L7Ae_Arch.
    InterProiIPR029064. L30e-like.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    IPR018492. Ribosomal_L7Ae/L8/Nhp2.
    IPR022481. Ribosomal_L7Ae_arc.
    IPR004037. Ribosomal_L7Ae_CS.
    IPR000948. Ribosomal_L7Ae_prok.
    [Graphical view]
    PfamiPF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view]
    PRINTSiPR00881. L7ARS6FAMILY.
    PR00884. RIBOSOMALHS6.
    SUPFAMiSSF55315. SSF55315. 1 hit.
    TIGRFAMsiTIGR03677. rpl7ae. 1 hit.
    PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8U160-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL    50
    VIIAEDVDPE EIVAHLPPLC EEKEIPYIYV PSKKELGAAA GIEVAAASVA 100
    IIEPGKARDL VEEIAMKVKE LMK 123
    Length:123
    Mass (Da):13,395
    Last modified:August 30, 2005 - v2
    Checksum:i934817F41BE2D4C5
    GO

    Sequence cautioni

    The sequence AAL81491.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81491.1. Different initiation.
    RefSeqiNP_579096.1. NC_003413.1.
    WP_011012514.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81491; AAL81491; PF1367.
    GeneIDi1469243.
    KEGGipfu:PF1367.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81491.1 . Different initiation.
    RefSeqi NP_579096.1. NC_003413.1.
    WP_011012514.1. NC_003413.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HVY X-ray 2.30 D 1-123 [» ]
    3HAX X-ray 2.11 D 2-123 [» ]
    3HAY X-ray 4.99 D 2-123 [» ]
    3HJW X-ray 2.35 C 3-122 [» ]
    3J20 electron microscopy 6.60 3 1-123 [» ]
    3J21 electron microscopy 6.60 4/G 1-123 [» ]
    3J43 electron microscopy 9.00 3 1-123 [» ]
    3J44 electron microscopy 9.00 G 1-123 [» ]
    3LWO X-ray 2.86 C 1-123 [» ]
    3LWP X-ray 2.50 C 1-123 [» ]
    3LWQ X-ray 2.68 C 1-123 [» ]
    3LWR X-ray 2.20 C 1-123 [» ]
    3LWV X-ray 2.50 C 1-123 [» ]
    3NMU X-ray 2.73 C/G 2-123 [» ]
    3NVI X-ray 2.71 B/D 2-123 [» ]
    3NVK X-ray 3.21 E/H 2-123 [» ]
    4BY9 NMR - D/G/J/M 3-123 [» ]
    ProteinModelPortali Q8U160.
    SMRi Q8U160. Positions 3-123.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48528N.
    STRINGi 186497.PF1367.

    Proteomic databases

    PRIDEi Q8U160.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL81491 ; AAL81491 ; PF1367 .
    GeneIDi 1469243.
    KEGGi pfu:PF1367.

    Phylogenomic databases

    eggNOGi COG1358.
    HOGENOMi HOG000055225.
    KOi K02936.
    OMAi YANSATF.

    Miscellaneous databases

    EvolutionaryTracei Q8U160.

    Family and domain databases

    Gene3Di 3.30.1330.30. 1 hit.
    HAMAPi MF_00326. Ribosomal_L7Ae_Arch.
    InterProi IPR029064. L30e-like.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    IPR018492. Ribosomal_L7Ae/L8/Nhp2.
    IPR022481. Ribosomal_L7Ae_arc.
    IPR004037. Ribosomal_L7Ae_CS.
    IPR000948. Ribosomal_L7Ae_prok.
    [Graphical view ]
    Pfami PF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view ]
    PRINTSi PR00881. L7ARS6FAMILY.
    PR00884. RIBOSOMALHS6.
    SUPFAMi SSF55315. SSF55315. 1 hit.
    TIGRFAMsi TIGR03677. rpl7ae. 1 hit.
    PROSITEi PS01082. RIBOSOMAL_L7AE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    2. "Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle."
      Xue S., Wang R., Yang F., Terns R.M., Terns M.P., Zhang X., Maxwell E.S., Li H.
      Mol. Cell 39:939-949(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.71 ANGSTROMS) IN COMPLEXES WITH FLPA NOP5 AND BOX C/D RNA, SUBUNIT.
    3. "Promiscuous behaviour of archaeal ribosomal proteins: implications for eukaryotic ribosome evolution."
      Armache J.P., Anger A.M., Marquez V., Franckenberg S., Frohlich T., Villa E., Berninghausen O., Thomm M., Arnold G.J., Beckmann R., Wilson D.N.
      Nucleic Acids Res. 41:1284-1293(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (6.6 ANGSTROMS) OF 70S RIBOSOME.

    Entry informationi

    Entry nameiRL7A_PYRFU
    AccessioniPrimary (citable) accession number: Q8U160
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 26, 2002
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3