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Q8U151

- RNP2_PYRFU

UniProt

Q8U151 - RNP2_PYRFU

Protein

Ribonuclease P protein component 2

Gene

rnp2

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. The RNA is catalytic, but its KM for pre-tRNA is 170-fold decreased in the presence of the 4 known protein subunits (Rnp1-4). The protein subunits also decrease the amount of Mg2+ needed for activity.3 PublicationsUniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.1 PublicationUniRule annotation

    Kineticsi

    kcat is 9.5 min(-1). For enzyme reconstituted with RNA and 4 known subunits (Rnp1-4).

    1. KM=0.18 µM for E.coli pre-tRNA(Tyr)1 Publication

    GO - Molecular functioni

    1. ribonuclease P activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA 5'-leader removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein component 2UniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P component 2UniRule annotation
    Alternative name(s):
    Pop5UniRule annotation
    Gene namesi
    Name:rnp2UniRule annotation
    Ordered Locus Names:PF1378
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. ribonuclease P complex Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 120120Ribonuclease P protein component 2PRO_0000140026Add
    BLAST

    Proteomic databases

    PRIDEiQ8U151.

    Interactioni

    Subunit structurei

    Consists of a catalytic RNA component and at least 4-5 protein subunits. Forms a subcomplex with Rnp3 which stimulates the catalytic RNA.4 PublicationsUniRule annotation

    Protein-protein interaction databases

    IntActiQ8U151. 1 interaction.
    STRINGi186497.PF1378.

    Structurei

    Secondary structure

    1
    120
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi16 – 2712
    Helixi31 – 5424
    Beta strandi57 – 626
    Turni63 – 664
    Beta strandi67 – 737
    Helixi74 – 763
    Helixi77 – 859
    Beta strandi93 – 10513
    Helixi106 – 1127
    Turni113 – 1186

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AV5X-ray3.15A/B/C/D/E1-120[»]
    ProteinModelPortaliQ8U151.
    SMRiQ8U151. Positions 15-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8U151.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic/archaeal RNase P protein component 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1369.
    HOGENOMiHOG000286243.
    KOiK03537.
    OMAiNPWLIDY.

    Family and domain databases

    HAMAPiMF_00755. RNase_P_2.
    InterProiIPR002759. RNase_P/MRP_subunit.
    IPR016434. RNase_P_comp-2.
    [Graphical view]
    PfamiPF01900. RNase_P_Rpp14. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004952. RNase_P_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8U151-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSERPKTLPP TLRDKKRYIA FKVISENQFN KDEIKEAIWN ACLRTLGELG    50
    TAKAKPWLIK FDETTQTGII RCDRNHVYDV IFSLTLVSDI NGNKAIIKVL 100
    GVSGTIKRLK RKFLSQFGWR 120
    Length:120
    Mass (Da):13,839
    Last modified:June 1, 2002 - v1
    Checksum:iB690DF4C26299915
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81502.1.
    RefSeqiNP_579107.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81502; AAL81502; PF1378.
    GeneIDi1469254.
    KEGGipfu:PF1378.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81502.1 .
    RefSeqi NP_579107.1. NC_003413.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2AV5 X-ray 3.15 A/B/C/D/E 1-120 [» ]
    ProteinModelPortali Q8U151.
    SMRi Q8U151. Positions 15-120.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8U151. 1 interaction.
    STRINGi 186497.PF1378.

    Proteomic databases

    PRIDEi Q8U151.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL81502 ; AAL81502 ; PF1378 .
    GeneIDi 1469254.
    KEGGi pfu:PF1378.

    Phylogenomic databases

    eggNOGi COG1369.
    HOGENOMi HOG000286243.
    KOi K03537.
    OMAi NPWLIDY.

    Miscellaneous databases

    EvolutionaryTracei Q8U151.

    Family and domain databases

    HAMAPi MF_00755. RNase_P_2.
    InterProi IPR002759. RNase_P/MRP_subunit.
    IPR016434. RNase_P_comp-2.
    [Graphical view ]
    Pfami PF01900. RNase_P_Rpp14. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004952. RNase_P_2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    2. "Functional reconstitution and characterization of Pyrococcus furiosus RNase P."
      Tsai H.Y., Pulukkunat D.K., Woznick W.K., Gopalan V.
      Proc. Natl. Acad. Sci. U.S.A. 103:16147-16152(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    3. "Cooperative RNP assembly: complementary rescue of structural defects by protein and RNA subunits of archaeal RNase P."
      Chen W.Y., Xu Y., Cho I.M., Oruganti S.V., Foster M.P., Gopalan V.
      J. Mol. Biol. 411:368-383(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    4. "Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors."
      Chen W.Y., Singh D., Lai L.B., Stiffler M.A., Lai H.D., Foster M.P., Gopalan V.
      Nucleic Acids Res. 40:4666-4680(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RNP3, SUBUNIT.
    5. Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS), INTERACTION WITH RNP3, SUBUNIT.
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

    Entry informationi

    Entry nameiRNP2_PYRFU
    AccessioniPrimary (citable) accession number: Q8U151
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3