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Protein

Ribonuclease P protein component 2

Gene

rnp2

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. The RNA is catalytic, but its KM for pre-tRNA is 170-fold decreased in the presence of the 4 known protein subunits (Rnp1-4). The protein subunits also decrease the amount of Mg2+ needed for activity.UniRule annotation3 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation1 Publication

Kineticsi

kcat is 9.5 min(-1). For enzyme reconstituted with RNA and 4 known subunits (Rnp1-4).

  1. KM=0.18 µM for E.coli pre-tRNA(Tyr)1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BRENDAi3.1.26.5. 5243.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein component 2UniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P component 2UniRule annotation
    Alternative name(s):
    Pop5UniRule annotation
    Gene namesi
    Name:rnp2UniRule annotation
    Ordered Locus Names:PF1378
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    Proteomesi
    • UP000001013 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 120120Ribonuclease P protein component 2PRO_0000140026Add
    BLAST

    Proteomic databases

    PRIDEiQ8U151.

    Interactioni

    Subunit structurei

    Consists of a catalytic RNA component and at least 4-5 protein subunits. Forms a subcomplex with Rnp3 which stimulates the catalytic RNA.UniRule annotation4 Publications

    Protein-protein interaction databases

    IntActiQ8U151. 1 interaction.
    STRINGi186497.PF1378.

    Structurei

    Secondary structure

    1
    120
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi16 – 2712Combined sources
    Helixi31 – 5424Combined sources
    Beta strandi57 – 626Combined sources
    Turni63 – 664Combined sources
    Beta strandi67 – 737Combined sources
    Helixi74 – 763Combined sources
    Helixi77 – 859Combined sources
    Beta strandi93 – 10513Combined sources
    Helixi106 – 1127Combined sources
    Turni113 – 1186Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AV5X-ray3.15A/B/C/D/E1-120[»]
    ProteinModelPortaliQ8U151.
    SMRiQ8U151. Positions 15-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8U151.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic/archaeal RNase P protein component 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiarCOG01365. Archaea.
    COG1369. LUCA.
    HOGENOMiHOG000286243.
    KOiK03537.
    OMAiNPWLIDY.

    Family and domain databases

    HAMAPiMF_00755. RNase_P_2. 1 hit.
    InterProiIPR002759. RNase_P/MRP_subunit.
    IPR016434. RNase_P_comp-2.
    [Graphical view]
    PfamiPF01900. RNase_P_Rpp14. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004952. RNase_P_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8U151-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSERPKTLPP TLRDKKRYIA FKVISENQFN KDEIKEAIWN ACLRTLGELG
    60 70 80 90 100
    TAKAKPWLIK FDETTQTGII RCDRNHVYDV IFSLTLVSDI NGNKAIIKVL
    110 120
    GVSGTIKRLK RKFLSQFGWR
    Length:120
    Mass (Da):13,839
    Last modified:June 1, 2002 - v1
    Checksum:iB690DF4C26299915
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81502.1.
    RefSeqiWP_011012525.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81502; AAL81502; PF1378.
    GeneIDi1469254.
    KEGGipfu:PF1378.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81502.1.
    RefSeqiWP_011012525.1. NC_003413.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AV5X-ray3.15A/B/C/D/E1-120[»]
    ProteinModelPortaliQ8U151.
    SMRiQ8U151. Positions 15-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ8U151. 1 interaction.
    STRINGi186497.PF1378.

    Proteomic databases

    PRIDEiQ8U151.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL81502; AAL81502; PF1378.
    GeneIDi1469254.
    KEGGipfu:PF1378.

    Phylogenomic databases

    eggNOGiarCOG01365. Archaea.
    COG1369. LUCA.
    HOGENOMiHOG000286243.
    KOiK03537.
    OMAiNPWLIDY.

    Enzyme and pathway databases

    BRENDAi3.1.26.5. 5243.

    Miscellaneous databases

    EvolutionaryTraceiQ8U151.

    Family and domain databases

    HAMAPiMF_00755. RNase_P_2. 1 hit.
    InterProiIPR002759. RNase_P/MRP_subunit.
    IPR016434. RNase_P_comp-2.
    [Graphical view]
    PfamiPF01900. RNase_P_Rpp14. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004952. RNase_P_2. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRNP2_PYRFU
    AccessioniPrimary (citable) accession number: Q8U151
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: June 1, 2002
    Last modified: November 11, 2015
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.