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Q8U151

- RNP2_PYRFU

UniProt

Q8U151 - RNP2_PYRFU

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Protein

Ribonuclease P protein component 2

Gene

rnp2

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. The RNA is catalytic, but its KM for pre-tRNA is 170-fold decreased in the presence of the 4 known protein subunits (Rnp1-4). The protein subunits also decrease the amount of Mg2+ needed for activity.3 PublicationsUniRule annotation

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.1 PublicationUniRule annotation

Kineticsi

kcat is 9.5 min(-1). For enzyme reconstituted with RNA and 4 known subunits (Rnp1-4).

  1. KM=0.18 µM for E.coli pre-tRNA(Tyr)1 Publication

GO - Molecular functioni

  1. ribonuclease P activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA 5'-leader removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P protein component 2UniRule annotation (EC:3.1.26.5UniRule annotation)
Short name:
RNase P component 2UniRule annotation
Alternative name(s):
Pop5UniRule annotation
Gene namesi
Name:rnp2UniRule annotation
Ordered Locus Names:PF1378
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. ribonuclease P complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120Ribonuclease P protein component 2PRO_0000140026Add
BLAST

Proteomic databases

PRIDEiQ8U151.

Interactioni

Subunit structurei

Consists of a catalytic RNA component and at least 4-5 protein subunits. Forms a subcomplex with Rnp3 which stimulates the catalytic RNA.4 PublicationsUniRule annotation

Protein-protein interaction databases

IntActiQ8U151. 1 interaction.
STRINGi186497.PF1378.

Structurei

Secondary structure

1
120
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 2712Combined sources
Helixi31 – 5424Combined sources
Beta strandi57 – 626Combined sources
Turni63 – 664Combined sources
Beta strandi67 – 737Combined sources
Helixi74 – 763Combined sources
Helixi77 – 859Combined sources
Beta strandi93 – 10513Combined sources
Helixi106 – 1127Combined sources
Turni113 – 1186Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AV5X-ray3.15A/B/C/D/E1-120[»]
ProteinModelPortaliQ8U151.
SMRiQ8U151. Positions 15-120.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U151.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic/archaeal RNase P protein component 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1369.
HOGENOMiHOG000286243.
KOiK03537.
OMAiNPWLIDY.

Family and domain databases

HAMAPiMF_00755. RNase_P_2.
InterProiIPR002759. RNase_P/MRP_subunit.
IPR016434. RNase_P_comp-2.
[Graphical view]
PfamiPF01900. RNase_P_Rpp14. 1 hit.
[Graphical view]
PIRSFiPIRSF004952. RNase_P_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8U151-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSERPKTLPP TLRDKKRYIA FKVISENQFN KDEIKEAIWN ACLRTLGELG
60 70 80 90 100
TAKAKPWLIK FDETTQTGII RCDRNHVYDV IFSLTLVSDI NGNKAIIKVL
110 120
GVSGTIKRLK RKFLSQFGWR
Length:120
Mass (Da):13,839
Last modified:June 1, 2002 - v1
Checksum:iB690DF4C26299915
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81502.1.
RefSeqiNP_579107.1. NC_003413.1.
WP_011012525.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81502; AAL81502; PF1378.
GeneIDi1469254.
KEGGipfu:PF1378.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81502.1 .
RefSeqi NP_579107.1. NC_003413.1.
WP_011012525.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2AV5 X-ray 3.15 A/B/C/D/E 1-120 [» ]
ProteinModelPortali Q8U151.
SMRi Q8U151. Positions 15-120.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8U151. 1 interaction.
STRINGi 186497.PF1378.

Proteomic databases

PRIDEi Q8U151.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL81502 ; AAL81502 ; PF1378 .
GeneIDi 1469254.
KEGGi pfu:PF1378.

Phylogenomic databases

eggNOGi COG1369.
HOGENOMi HOG000286243.
KOi K03537.
OMAi NPWLIDY.

Miscellaneous databases

EvolutionaryTracei Q8U151.

Family and domain databases

HAMAPi MF_00755. RNase_P_2.
InterProi IPR002759. RNase_P/MRP_subunit.
IPR016434. RNase_P_comp-2.
[Graphical view ]
Pfami PF01900. RNase_P_Rpp14. 1 hit.
[Graphical view ]
PIRSFi PIRSF004952. RNase_P_2. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Functional reconstitution and characterization of Pyrococcus furiosus RNase P."
    Tsai H.Y., Pulukkunat D.K., Woznick W.K., Gopalan V.
    Proc. Natl. Acad. Sci. U.S.A. 103:16147-16152(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  3. "Cooperative RNP assembly: complementary rescue of structural defects by protein and RNA subunits of archaeal RNase P."
    Chen W.Y., Xu Y., Cho I.M., Oruganti S.V., Foster M.P., Gopalan V.
    J. Mol. Biol. 411:368-383(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  4. "Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors."
    Chen W.Y., Singh D., Lai L.B., Stiffler M.A., Lai H.D., Foster M.P., Gopalan V.
    Nucleic Acids Res. 40:4666-4680(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RNP3, SUBUNIT.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS), INTERACTION WITH RNP3, SUBUNIT.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiRNP2_PYRFU
AccessioniPrimary (citable) accession number: Q8U151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3