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Protein

Uridylate kinase

Gene

pyrH

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor.1 Publication

Catalytic activityi

ATP + UMP = ADP + UDP.

Enzyme regulationi

Inhibited by UTP.By similarity

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi6Magnesium 11 Publication1
Binding sitei44UMP; via amide nitrogen1 Publication1
Binding sitei45ATP; via amide nitrogen1
Binding sitei49ATPBy similarity1
Binding sitei66UMP1 Publication1
Metal bindingi120Magnesium 11 Publication1
Metal bindingi121Magnesium 11 Publication1
Metal bindingi121Magnesium 21 Publication1
Binding sitei140ATP1
Binding sitei141ATP1
Binding sitei146ATP; via amide nitrogen and carbonyl oxygen1
Binding sitei149ATP1
Binding sitei179UMP; via amide nitrogen1 Publication1
Metal bindingi182Magnesium 2; via carbonyl oxygen1 Publication1
Binding sitei182ATP; via amide nitrogen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 10ATP2
Nucleotide bindingi114 – 120UMP1 Publication7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.4.22. 5243.
UniPathwayiUPA00159; UER00275.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinase (EC:2.7.4.22)
Short name:
UK
Alternative name(s):
Uridine monophosphate kinase
Short name:
UMP kinase
Short name:
UMPK
Gene namesi
Name:pyrH
Ordered Locus Names:PF1407
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001439231 – 225Uridylate kinaseAdd BLAST225

Proteomic databases

PRIDEiQ8U122.

Interactioni

Subunit structurei

Homohexamer; trimer of dimers.2 Publications

Protein-protein interaction databases

STRINGi186497.PF1407.

Structurei

Secondary structure

1225
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi9 – 12Combined sources4
Beta strandi14 – 16Combined sources3
Helixi19 – 35Combined sources17
Beta strandi36 – 42Combined sources7
Helixi45 – 56Combined sources12
Turni57 – 59Combined sources3
Helixi62 – 84Combined sources23
Helixi85 – 87Combined sources3
Helixi96 – 104Combined sources9
Beta strandi115 – 118Combined sources4
Helixi120 – 130Combined sources11
Beta strandi134 – 139Combined sources6
Beta strandi141 – 144Combined sources4
Beta strandi146 – 148Combined sources3
Turni150 – 152Combined sources3
Beta strandi160 – 162Combined sources3
Helixi164 – 172Combined sources9
Beta strandi182 – 184Combined sources3
Helixi186 – 195Combined sources10
Beta strandi199 – 202Combined sources4
Helixi204 – 207Combined sources4
Helixi210 – 214Combined sources5
Beta strandi218 – 223Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BMUX-ray2.55A/B1-225[»]
2BRIX-ray3.00A/B1-225[»]
2BRXX-ray2.40A/B1-225[»]
2JI5X-ray2.45A/B1-225[»]
ProteinModelPortaliQ8U122.
SMRiQ8U122.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U122.

Family & Domainsi

Sequence similaritiesi

Belongs to the UMP kinase family.Curated

Phylogenomic databases

eggNOGiarCOG00858. Archaea.
COG0528. LUCA.
HOGENOMiHOG000047188.
KOiK09903.
OMAiYIGIHIT.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_A. PyrH_A. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR011818. Uridylate_kinase_arch/spir.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02076. pyrH_arch. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8U122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIVFDIGGS VLVPENPDID FIKEIAYQLT KVSEDHEVAV VVGGGKLARK
60 70 80 90 100
YIEVAEKFNS SETFKDFIGI QITRANAMLL IAALREKAYP VVVEDFWEAW
110 120 130 140 150
KAVQLKKIPV MGGTHPGHTT DAVAALLAEF LKADLLVVIT NVDGVYTADP
160 170 180 190 200
KKDPTAKKIK KMKPEELLEI VGKGIEKAGS SSVIDPLAAK IIARSGIKTI
210 220
VIGKEDAKDL FRVIKGDHNG TTIEP
Length:225
Mass (Da):24,491
Last modified:June 1, 2002 - v1
Checksum:i510D151A02F8D534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81531.1.
RefSeqiWP_011012554.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81531; AAL81531; PF1407.
GeneIDi1469283.
KEGGipfu:PF1407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81531.1.
RefSeqiWP_011012554.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BMUX-ray2.55A/B1-225[»]
2BRIX-ray3.00A/B1-225[»]
2BRXX-ray2.40A/B1-225[»]
2JI5X-ray2.45A/B1-225[»]
ProteinModelPortaliQ8U122.
SMRiQ8U122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1407.

Proteomic databases

PRIDEiQ8U122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81531; AAL81531; PF1407.
GeneIDi1469283.
KEGGipfu:PF1407.

Phylogenomic databases

eggNOGiarCOG00858. Archaea.
COG0528. LUCA.
HOGENOMiHOG000047188.
KOiK09903.
OMAiYIGIHIT.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275.
BRENDAi2.7.4.22. 5243.

Miscellaneous databases

EvolutionaryTraceiQ8U122.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_A. PyrH_A. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR011818. Uridylate_kinase_arch/spir.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02076. pyrH_arch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYRH_PYRFU
AccessioniPrimary (citable) accession number: Q8U122
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.