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Protein

Membrane-bound hydrogenase subunit alpha

Gene

mbhL

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha subunit of a hydrogen-evolving hydrogenase that utilizes protons both as a substrate for hydrogen production and proton translocation. Acts by coupling the redox reaction via ferredoxin and iron-sulfur (Fe-S) clusters to proton translocation across the membrane thereby conserving the redox energy in a proton gradient.3 Publications

Caution

The subunit composition of membrane-bound hydrogenase complex is currently unclear. It has been shown to be a heterodimer of MbhK and MbhL (PubMed:10852873). Other studies have shown it to contain MbhJ in addition to MbhK and MbhL (PubMed:11054105).2 Publications

Catalytic activityi

H2 + 2 oxidized ferredoxin = 2 reduced ferredoxin + 2 H+.3 Publications

Cofactori

Ni2+1 PublicationNote: Binds 1 nickel ion per mole of protein.1 Publication

Enzyme regulationi

Inhibited by 0.1 mM Cu2+.1 Publication

Kineticsi

Measured for the whole complex.1 Publication
  1. KM=36 µM for ferredoxin2 Publications

    pH dependencei

    Optimum pH is 7.0. Active between pH 6.0-8.5.2 Publications

    Temperature dependencei

    Optimum temperature is 90 degrees Celsius for membrane-bound enzyme but 80 degrees Celsius for the purified enzyme. Membrane-bound enzyme has a half-life of 2h at 100 degrees Celsius whereas the half-life of the purified enzyme is 30 minutes at 100 degrees Celsius.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi68NickelBy similarity1
    Metal bindingi71NickelBy similarity1
    Metal bindingi374NickelBy similarity1
    Metal bindingi377NickelBy similarity1

    GO - Molecular functioni

    Keywordsi

    Molecular functionOxidoreductase
    LigandMetal-binding, Nickel

    Enzyme and pathway databases

    BRENDAi1.12.7.2 5243

    Protein family/group databases

    TCDBi3.D.1.4.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Membrane-bound hydrogenase subunit alpha1 Publication (EC:1.12.7.21 Publication)
    Short name:
    MBH-alpha1 Publication
    Gene namesi
    Name:mbhL1 Publication
    Synonyms:mbh121 Publication
    Ordered Locus Names:PF1434
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    Proteomesi
    • UP000001013 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004207911 – 427Membrane-bound hydrogenase subunit alphaAdd BLAST427

    Proteomic databases

    PRIDEiQ8U0Z6

    Interactioni

    Subunit structurei

    The membrane-bound hydrogenase complex is composed of MbhK and MbhL, and may also contain MbhJ.1 Publication

    Protein-protein interaction databases

    STRINGi186497.PF1434

    Structurei

    3D structure databases

    ProteinModelPortaliQ8U0Z6
    SMRiQ8U0Z6
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 49 kDa subunit family.Curated

    Phylogenomic databases

    eggNOGiarCOG01547 Archaea
    COG3261 LUCA
    HOGENOMiHOG000228263
    KOiK18016
    OMAiASIDPCL
    OrthoDBiPOG093Z02AQ

    Family and domain databases

    Gene3Di1.10.645.20, 1 hit
    InterProiView protein in InterPro
    IPR001135 NADH_Q_OxRdtase_suD
    IPR038290 NADH_Q_OxRdtase_suD_sf
    IPR001501 Ni-dep_hyd_lsu
    IPR029014 NiFe-Hase_large
    PfamiView protein in Pfam
    PF00346 Complex1_49kDa, 1 hit
    PF00374 NiFeSe_Hases, 1 hit
    SUPFAMiSSF56762 SSF56762, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q8U0Z6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKKVEYWVKI PFGPIHPGLE EPEKFIITLD GERIVNVDVK LGYNLRGVQW
    60 70 80 90 100
    IGMRRNYVQI MYLAERMCGI CSFSHNHTYV RAVEEMAGIE VPERAEYIRV
    110 120 130 140 150
    IVGELERIHS HLLNLGVVGH DIGYDTVLHL TWLARERVMD VLEAVSGNRV
    160 170 180 190 200
    NYSMVTIGGV RRDIGEKQKR LILDMIKYYR EVLPQIEDVF LHDSTIEARL
    210 220 230 240 250
    RDVAVVPKKL AIEMGAVGPT ARGSGIKEDS RWSEQLGVYP DLGIKPVTPE
    260 270 280 290 300
    DVTGEKARGD VYDRMAVRIG ELWMSLDLLE HALDQMPEGK IKTFPKDNIL
    310 320 330 340 350
    VAKLKLLGDG EGIGRYEAPR GELVHYVRGQ KGRDGPVRWK PREPTFPNLF
    360 370 380 390 400
    TIAKALEGNE LADLVVAIAS IDPCLSCTDR VAIVKEGKKV VLTEKDLLKL
    410 420
    SIEKTKEINP NVKGDPTPTG IGCSRGV
    Length:427
    Mass (Da):47,934
    Last modified:June 1, 2002 - v1
    Checksum:iC7083303E5502B92
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE009950 Genomic DNA Translation: AAL81558.1
    RefSeqiWP_011012581.1, NC_003413.1

    Genome annotation databases

    EnsemblBacteriaiAAL81558; AAL81558; PF1434
    GeneIDi1469310
    KEGGipfu:PF1434
    PATRICifig|186497.12.peg.1496

    Similar proteinsi

    Entry informationi

    Entry nameiMBHLA_PYRFU
    AccessioniPrimary (citable) accession number: Q8U0Z6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
    Last sequence update: June 1, 2002
    Last modified: March 28, 2018
    This is version 86 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

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