Reviewed, UniProtKB/Swiss-Prot Q8U0R9 (GUAAA_PYRFU)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit A
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	PF1515

Organism

Pyrococcus furiosus [Complete proteome] [HAMAP]

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140229
Regions
	Domain	1 – 188	188	Glutamine amidotransferase type-1
Sites
	Active site	78	1	Nucleophile By similarity
	Active site	165	1	By similarity
	Active site	167	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U0R9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: B9E5ADC7CF832EBF	FASTA	188	21,306
	10 20 30 40 50 60 MIIIMDNGGQ YVHRIWRTLR YLGVEAKIIP NTTPLDEIKA MKPKGIIFSG GPSLENTGNC 70 80 90 100 110 120 EKILEHYEEF NVPILGICLG HQLIAKFFGG EVGRGEKAEY SLVEIEIIDE NDIFKGLPRK 130 140 150 160 170 180 VRVWESHMDE VKKLPPKFKL LARSDTCPVE AMKHEELPIY GVQFHPEVAH TEHGADILRN FAKICGEL

References

[1]	"The complete sequence of the Pyrococcus furiosus genome." Weiss R.B., Dunn D.M., Robb F.T., Brown J.R. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009950 Genomic DNA. Translation: AAL81639.1.
RefSeq	NP_579244.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
SMR	Q8U0R9. Positions 1-187.
ModBase	Search...
Genome annotation databases
GeneID	1469391.
GenomeReviews	Gene locus PF1515 in contig AE009950_GR.
KEGG	pfu:PF1515.
NMPDR	fig|186497.1.peg.1562.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8U0R9.
OMA	GQYVHRI.
Enzyme and pathway databases
BRENDA	6.3.5.2. 321.
Family and domain databases
HAMAP	MF_01510. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR004739. GMP_synth_N. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_PYRFU

Accession

Primary (citable) accession number: Q8U0R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)