Q8U0R8 (GUAAB_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	PF1516

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140248
Regions
	Domain	6 – 190	185	GMPS ATP-PPase
	Nucleotide binding	33 – 39	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U0R8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 8BFAF701EBC3CD3F	FASTA	313	35,282
	10 20 30 40 50 60 MKRWVKVWEK FIEEKIREIR ETVGDSKAII ALSGGVDSST AAVLAHRAIG DRLHAVFVNT 70 80 90 100 110 120 GFLRKGEPEF VIKTFRDEFG MNLHYVDAQD RFFAALKGVT DPEEKRKIIG RVFIEVFEEV 130 140 150 160 170 180 AKDIGAEYLI QGTIAPDWIE SQGKIKSHHN VGGLPEKLNL KIIEPLRDLY KDEVRQLAKE 190 200 210 220 230 240 LGLPEKIYNR MPFPGPGLAV RVIGEVTPEK IRIVREANAI VEEEIEKAGL RPWQAFAVLL 250 260 270 280 290 300 GVKTVGVQGD IRAYKETIAV RIVESVDGMT ANAMNVPWEV LQRIAFRITS EIPEVGRVLY 310 DITNKPPATI EFE

References

[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL81640.1.
RefSeq	NP_579245.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U0R8.
SMR	Q8U0R8. Positions 8-313.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000004306; EBPYRP00000004167; EBPYRG00000004306.
GeneID	1469392.
GenomeReviews	Gene locus PF1516 in contig AE009950_GR.
KEGG	pfu:PF1516.
NMPDR	fig|186497.1.peg.1563.
Phylogenomic databases
GeneTree	EBGT00050000022268.
HOGENOM	HBG391775.
OMA	QGTIAPD.
PhylomeDB	Q8U0R8.
ProtClustDB	PRK00919.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_PYRFU

Accession

Primary (citable) accession number: Q8U0R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program