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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADUniRule annotation1
Binding sitei62NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei126NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi154Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi169IronUniRule annotation1
Binding sitei217NAD; via amide nitrogenUniRule annotation1
Binding sitei227GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 55NADUniRule annotation2
Nucleotide bindingi152 – 154NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:PF1530
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539521 – 252Thiamine thiazole synthaseAdd BLAST252

Proteomic databases

PRIDEiQ8U0Q5.

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi186497.PF1530.

Structurei

3D structure databases

ProteinModelPortaliQ8U0Q5.
SMRiQ8U0Q5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OMAiMFPRIVV.
OrthoDBiPOG093Z09PU.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8U0Q5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKDVVISRA IVESYFKDLL NNLELDVAIV GAGPSGMVAG YYLAKGGAKV
60 70 80 90 100
AIFEKKLSIG GGIWGGGMGF NKIVVQEEAK EILDEFDIRY EEFEKGYYVA
110 120 130 140 150
DAIEVATTIA SKTVKAGVKI FNMVEVEDLV VKDDRVSGIV INWTPVKMTG
160 170 180 190 200
LHVDPLTVEA KYVIDSTGHG AQVTQFLLKR GLIEKIPGEG AMWAEMGEKL
210 220 230 240 250
TVENTKEVFP GLYVTGMAAN AVSGAPRMGP IFGGMFLSGR KAAMEILQKL

GL
Length:252
Mass (Da):27,157
Last modified:June 1, 2002 - v1
Checksum:iB91920C50B46F3FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81654.1.
RefSeqiWP_011012677.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81654; AAL81654; PF1530.
GeneIDi1469406.
KEGGipfu:PF1530.
PATRICifig|186497.12.peg.1595.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTHI4_PYRFU
AccessioniPrimary (citable) accession number: Q8U0Q5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 2002
Last modified: June 7, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families