Q8U0N7 (RTCA_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	PF1549

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156432
Regions
	Nucleotide binding	283 – 287	5	ATP By similarity
Sites
	Active site	307	1	Tele-AMP-histidine intermediate By similarity
	Binding site	100	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8U0N7 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D4D2E5898CB3B4BE	FASTA	342	36,804
	10 20 30 40 50 60 MIIIDGSYGE GGGQILRTSI ALSAITGEPV KIINIRANRP NPGLRPQHLN AILALKKLAN 70 80 90 100 110 120 AKVEGAEVGS REVTFIPGEL KGGEIRVDIG TAGSITLVLQ ALLPAMVFAK DTVEFKITGG 130 140 150 160 170 180 TDVSWSPPVD YLINVTMFAL RKIGIEGEIK LLRRGHYPKG GGIVAGYVKP WIERKELIAE 190 200 210 220 230 240 EFENIYKVSG ISHATNLPAH VAERQAKAAM EELKVLGVPI EIKKEVSHSL GPGSGIVVWA 250 260 270 280 290 300 ETECLRLGGD ALGKKGKPAE EVGREAAQEL LSQVKTKACV DKFLGDQIIP FLAISGGKIK 310 320 330 340 VAEITKHLIT NVWVVEQFFG KVFEVKGGVG EKGEVRVVRK AW

References

[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009950 Genomic DNA. Translation: AAL81673.1.
RefSeq	NP_579278.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q8U0N7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000006026; EBPYRP00000005887; EBPYRG00000006025.
GeneID	1469425.
GenomeReviews	Gene locus PF1549 in contig AE009950_GR.
KEGG	pfu:PF1549.
NMPDR	fig|186497.1.peg.1596.
Phylogenomic databases
GeneTree	EBGT00050000022634.
HOGENOM	HBG683539.
OMA	HYLALVW.
PhylomeDB	Q8U0N7.
ProtClustDB	PRK04204.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_PYRFU

Accession

Primary (citable) accession number: Q8U0N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program