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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtc

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.1 Publication

Catalytic activityi

GTP + RNA 3'-terminal-phosphate = GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.1 Publication

Enzyme regulationi

Inhibited by GMP.1 Publication

Temperature dependencei

Optimum temperature is 95 degrees Celsius.1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.51 Publication)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Alternative name(s):
GTP-dependent RNA 3'-terminal-phosphate cyclaseCurated
Gene namesi
Name:rtc1 Publication
Synonyms:rtcAUniRule annotation
Ordered Locus Names:PF1549
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342RNA 3'-terminal phosphate cyclasePRO_0000156432Add
BLAST

Proteomic databases

PRIDEiQ8U0N7.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF1549.

Structurei

3D structure databases

ProteinModelPortaliQ8U0N7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK18105.
OMAiMGDQIIP.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U0N7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIIIDGSYGE GGGQILRTSI ALSAITGEPV KIINIRANRP NPGLRPQHLN
60 70 80 90 100
AILALKKLAN AKVEGAEVGS REVTFIPGEL KGGEIRVDIG TAGSITLVLQ
110 120 130 140 150
ALLPAMVFAK DTVEFKITGG TDVSWSPPVD YLINVTMFAL RKIGIEGEIK
160 170 180 190 200
LLRRGHYPKG GGIVAGYVKP WIERKELIAE EFENIYKVSG ISHATNLPAH
210 220 230 240 250
VAERQAKAAM EELKVLGVPI EIKKEVSHSL GPGSGIVVWA ETECLRLGGD
260 270 280 290 300
ALGKKGKPAE EVGREAAQEL LSQVKTKACV DKFLGDQIIP FLAISGGKIK
310 320 330 340
VAEITKHLIT NVWVVEQFFG KVFEVKGGVG EKGEVRVVRK AW
Length:342
Mass (Da):36,804
Last modified:June 1, 2002 - v1
Checksum:iD4D2E5898CB3B4BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81673.1.
RefSeqiNP_579278.1. NC_003413.1.
WP_011012696.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81673; AAL81673; PF1549.
GeneIDi1469425.
KEGGipfu:PF1549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81673.1.
RefSeqiNP_579278.1. NC_003413.1.
WP_011012696.1. NC_003413.1.

3D structure databases

ProteinModelPortaliQ8U0N7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1549.

Proteomic databases

PRIDEiQ8U0N7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81673; AAL81673; PF1549.
GeneIDi1469425.
KEGGipfu:PF1549.

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK18105.
OMAiMGDQIIP.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "GTP-dependent RNA 3'-terminal phosphate cyclase from the hyperthermophilic archaeon Pyrococcus furiosus."
    Sato A., Soga T., Igarashi K., Takesue K., Tomita M., Kanai A.
    Genes Cells 16:1190-1199(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, RNA-BINDING, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiRTCA_PYRFU
AccessioniPrimary (citable) accession number: Q8U0N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: February 4, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Generally, RNA 3'-terminal phosphate cyclases are ATP dependent, but P.furiosus enzyme preferentially uses GTP rather than ATP.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.