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Protein

Tryptophan synthase beta chain 2

Gene

trpB2

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.By similarity

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain 2 (trpB2), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain 1 (trpB1)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain 2 (EC:4.2.1.20)
Gene namesi
Name:trpB2
Ordered Locus Names:PF1592
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Tryptophan synthase beta chain 2PRO_0000099051Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei110 – 1101N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PRIDEiQ8U0J5.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.By similarity

Protein-protein interaction databases

STRINGi186497.PF1592.

Structurei

3D structure databases

ProteinModelPortaliQ8U0J5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Phylogenomic databases

eggNOGiarCOG01432. Archaea.
COG1350. LUCA.
HOGENOMiHOG000161709.
KOiK06001.
OMAiNHVMLHQ.

Family and domain databases

HAMAPiMF_00133. Trp_synth_beta.
InterProiIPR006316. Trp_synth_b-like.
IPR006653. Trp_synth_b_CS.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PANTHERiPTHR10314:SF21. PTHR10314:SF21. 2 hits.
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
PIRSF500824. TrpB_prok. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01415. trpB_rel. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U0J5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVVLPDGRI PRRWYNILPD LPEPLAPPLD PETNEPVDPK KLERIFAKEL
60 70 80 90 100
VKQEMSTKRY IKIPEEVRKM YSKIGRPTPL FRATNLEKYL NTPARIYFKF
110 120 130 140 150
EGATVTGSHK INTALAQAYY AKKEGIERLV TETGAGQWGT ALSLAGALMG
160 170 180 190 200
IKVRVYMARA SYEQKPYRKV LMRIYGAEVF PSPSENTEIG KRFLSENPNH
210 220 230 240 250
PGSLGIAISE AIEDVLKDEK ARYSLGSVLN HVLMHQTVIG LEAKQQMEEF
260 270 280 290 300
EEPDVIIGCV GGGSNFAGLA YPFVKEVLDG DNEYEFIAVE PKAAPSMTRG
310 320 330 340 350
VYTYDFGDSG ELTPKLKMHT LGHRYHVPPI HAGGLRYHGV APTLSVLVNN
360 370 380 390 400
GIVKPIAYHQ TEVFEAAALF AKLEGIVPAP ESAHAIKATI DKAIEAKREG
410 420 430 440
KEIVILFNLS GHGLLDLHGY EEYLEGRLQD YEPKDLPISN PLNPKP
Length:446
Mass (Da):49,616
Last modified:June 1, 2002 - v1
Checksum:i11564BFD6C41A64F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81716.1.
RefSeqiWP_011012738.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81716; AAL81716; PF1592.
GeneIDi1469468.
KEGGipfu:PF1592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81716.1.
RefSeqiWP_011012738.1. NC_003413.1.

3D structure databases

ProteinModelPortaliQ8U0J5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1592.

Proteomic databases

PRIDEiQ8U0J5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81716; AAL81716; PF1592.
GeneIDi1469468.
KEGGipfu:PF1592.

Phylogenomic databases

eggNOGiarCOG01432. Archaea.
COG1350. LUCA.
HOGENOMiHOG000161709.
KOiK06001.
OMAiNHVMLHQ.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Family and domain databases

HAMAPiMF_00133. Trp_synth_beta.
InterProiIPR006316. Trp_synth_b-like.
IPR006653. Trp_synth_b_CS.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PANTHERiPTHR10314:SF21. PTHR10314:SF21. 2 hits.
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
PIRSF500824. TrpB_prok. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01415. trpB_rel. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiTRPB2_PYRFU
AccessioniPrimary (citable) accession number: Q8U0J5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.