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Protein

AMP phosphorylase

Gene

PF1607

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO.UniRule annotation

Catalytic activityi

AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate.UniRule annotation
CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate.UniRule annotation
UMP + phosphate = uracil + alpha-D-ribose 1,5-bisphosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei168 – 1681AMP; via amide nitrogenUniRule annotation
Binding sitei203 – 2031AMP; via amide nitrogenUniRule annotation
Active sitei256 – 2561Proton donorUniRule annotation
Binding sitei264 – 2641AMPUniRule annotation
Binding sitei288 – 2881AMPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi194 – 1996AMPUniRule annotation

GO - Molecular functioni

  1. AMP binding Source: UniProtKB-HAMAP
  2. phosphorylase activity Source: InterPro
  3. thymidine phosphorylase activity Source: InterPro

GO - Biological processi

  1. AMP catabolic process Source: UniProtKB-HAMAP
  2. pyrimidine deoxyribonucleoside metabolic process Source: InterPro
  3. pyrimidine nucleobase metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
AMP phosphorylaseUniRule annotation (EC:2.4.2.57UniRule annotation)
Short name:
AMPpaseUniRule annotation
Alternative name(s):
Nucleoside monophosphate phosphorylaseUniRule annotation
Short name:
NMP phosphorylaseUniRule annotation
Gene namesi
Ordered Locus Names:PF1607
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503AMP phosphorylasePRO_0000059091Add
BLAST

Proteomic databases

PRIDEiQ8U0I2.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF1607.

Structurei

3D structure databases

ProteinModelPortaliQ8U0I2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0213.
HOGENOMiHOG000252767.
KOiK00758.
OMAiDWVVDAY.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
HAMAPiMF_02132. AMP_phosphorylase.
InterProiIPR017713. AMP_phosphorylase.
IPR009010. Asp_de-COase-like_dom.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR000053. Pyrmidine_PPase.
IPR017872. Pyrmidine_PPase_CS.
IPR013466. Thymidine/AMP_Pase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000478. TP_PyNP. 1 hit.
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF50692. SSF50692. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR03327. AMP_phos. 1 hit.
TIGR02645. ARCH_P_rylase. 1 hit.
PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U0I2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGKIKILDI ETGNLAIFIN PEDAEQWRIH PNDLVKIESG KRYIYGSAFI
60 70 80 90 100
GNIVEKGEIG ISKDVLSIHQ FSNGEIVSLS PAGTPESVKY IKKKMRGEKL
110 120 130 140 150
KKVEIETIVR DIVDRKLRNT EISAFVSAIE INGLDMEEIA ALTIAMAETG
160 170 180 190 200
DMLDIERKPI MDIHSIGGVP GNKTNVIVVP IVAAAGLTIP KTSSRAITSA
210 220 230 240 250
AGTADVVEVL TNVTLTLEEI KRIVEKIGAC LVWGGALNLA PADDLMIHVE
260 270 280 290 300
RRLSLDPRGL MLASIMAKKY AIGSQYILID IPTGKGAKVE SMEEARSLAR
310 320 330 340 350
DFIELGKRLG QYVEVAITYG GQPIGYTVGP ALEAKEALET LMTGRGPGSL
360 370 380 390 400
VEKAIGLAGL LLEMGGAAPK GKGKIIAREI LEKGKAYQKM REIIEEQGGD
410 420 430 440 450
PDIKPEDIPI GDKTYTIHAQ TNGYVTAIDN RGITAIAREA GAPEDKGAGI
460 470 480 490 500
RLHVKVGDKV KEGDPLFTIH AESESRLDKA IVLARRLEPI KIEGMVLQVI

ENL
Length:503
Mass (Da):54,340
Last modified:June 1, 2002 - v1
Checksum:iDB90216833A12512
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81731.1.
RefSeqiNP_579336.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81731; AAL81731; PF1607.
GeneIDi1469483.
KEGGipfu:PF1607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81731.1.
RefSeqiNP_579336.1. NC_003413.1.

3D structure databases

ProteinModelPortaliQ8U0I2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1607.

Proteomic databases

PRIDEiQ8U0I2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81731; AAL81731; PF1607.
GeneIDi1469483.
KEGGipfu:PF1607.

Phylogenomic databases

eggNOGiCOG0213.
HOGENOMiHOG000252767.
KOiK00758.
OMAiDWVVDAY.

Family and domain databases

Gene3Di3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
HAMAPiMF_02132. AMP_phosphorylase.
InterProiIPR017713. AMP_phosphorylase.
IPR009010. Asp_de-COase-like_dom.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR000053. Pyrmidine_PPase.
IPR017872. Pyrmidine_PPase_CS.
IPR013466. Thymidine/AMP_Pase.
[Graphical view]
PANTHERiPTHR10515. PTHR10515. 1 hit.
PfamiPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000478. TP_PyNP. 1 hit.
SMARTiSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMiSSF47648. SSF47648. 1 hit.
SSF50692. SSF50692. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsiTIGR03327. AMP_phos. 1 hit.
TIGR02645. ARCH_P_rylase. 1 hit.
PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiAMPPA_PYRFU
AccessioniPrimary (citable) accession number: Q8U0I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.