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Q8U0H4 (RTCB_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing ligase RtcB

EC=6.5.1.-

Cleaved into the following chain:

  1. Pfu hyp2 intein
    EC=3.1.-.-
Gene names
Name:rtcB
Ordered Locus Names:PF1615
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length970 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

tRNA-splicing ligase that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs By similarity.

Subunit structure

Monomer By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the RtcB family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 105105tRNA-splicing ligase RtcB, 1st part Potential
PRO_0000232532
Chain106 – 586481Pfu hyp2 intein Potential
PRO_0000232533
Chain587 – 970384tRNA-splicing ligase RtcB, 2nd part Potential
PRO_0000232534

Regions

Domain256 – 420165DOD-type homing endonuclease

Sites

Metal binding5871Zinc Potential
Metal binding6921Zinc Potential
Metal binding7231Zinc Potential

Sequences

Sequence LengthMass (Da)Tools
Q8U0H4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: CC0AEE4573CFE7C2

FASTA970109,712
        10         20         30         40         50         60 
MIILRVVNVA VPLKRIDKIR WEIPKFDKRM KVPGRVYADD VLIEKMRQDR TLEQAANVAM 

        70         80         90        100        110        120 
LPGIYKYSIV MPDGHQGYGF PIGGVAAFDI KEGVISPGGI GYDINCLAPG TKVLTEHGYW 

       130        140        150        160        170        180 
LKIEEMPEKF KLQRLRLYNI EEGHNDFSRV AFVAERNIEK DETAIRIVTE TGTLIEGSED 

       190        200        210        220        230        240 
HPVLTPQGYV YLKNIKEGDY VIVYPFEGVP YEEKKGIIID ESAFEGEDPQ VIKFLKERNL 

       250        260        270        280        290        300 
LPLRWEDPKI GTLARILGFA LGDGHLGEMG GRLVLAFYGR EETLRELKKD LESLGIKANL 

       310        320        330        340        350        360 
YVREKNYRIK TESGEYSGKT VLAELRVSSR SFALLLEKLG MPRGEKTKKA YRIPVWIMEA 

       370        380        390        400        410        420 
PLWVKRNFLA GFFGADGSIV EFKGTTPLPI HLTQAKDVAL EENLKEFLYD ISRILEEFGV 

       430        440        450        460        470        480 
KTTIYKVNSK KSVTYRLSIV GEENIRNFLG KINYEYDPKK KAKGLIAYAY LKFKESVKKE 

       490        500        510        520        530        540 
RRKAMEISKK IYEETGNIDR AYKAVKDIVN RRFVERTIYE GERNPRVPKN FLTFEEFAKE 

       550        560        570        580        590        600 
RGYEGGFVAE KVVKVERIKP EYDRFYDIGV YHEAHNFIAN GIVVHNCGVR LIRTNLTEKD 

       610        620        630        640        650        660 
VRPKIKQLVD TLFKNVPSGV GSQGKVRLHW TQIDDVLVDG AKWAVDQGYG WERDLERLEE 

       670        680        690        700        710        720 
GGRMEGADPD AVSQRAKQRG APQLGSLGSG NHFLEVQVVD KIFDEEIAKA YGLFEGQVVV 

       730        740        750        760        770        780 
MVHTGSRGLG HQVASDYLRI MERAIRKYGI PWPDRELVSV PFQSEEGQRY FSAMKAAANF 

       790        800        810        820        830        840 
AWANRQMITH WVRESFQEVF RQDPEGDLGM EIVYDVAHNI GKVEEHEVDG KKVKVIVHRK 

       850        860        870        880        890        900 
GATRAFPPGH EAIPKIYRDV GQPVLIPGSM GTASYVLAGT EGAMAETFGS TCHGAGRVLS 

       910        920        930        940        950        960 
RAAATRQYRG DRIRDELLRR GIYVRAASMR VVAEEAPGAY KNVDNVVKVV SEAGIAKLVA 

       970 
RMRPIGVAKG 

« Hide

References

[1]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009950 Genomic DNA. Translation: AAL81739.1.
RefSeqNP_579344.1. NC_003413.1.

3D structure databases

ProteinModelPortalQ8U0H4.
SMRQ8U0H4. Positions 11-106, 586-970.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8U0H4. 1 interaction.
STRING186497.PF1615.

Proteomic databases

PRIDEQ8U0H4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL81739; AAL81739; PF1615.
GeneID1469492.
KEGGpfu:PF1615.

Phylogenomic databases

eggNOGCOG1372.
HOGENOMHOG000296282.
KOK14415.
OMAVHNCGVR.

Family and domain databases

Gene3D2.170.16.10. 3 hits.
3.10.28.10. 2 hits.
InterProIPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR001233. RtcB.
[Graphical view]
PANTHERPTHR11118. PTHR11118. 1 hit.
PfamPF14528. LAGLIDADG_3. 1 hit.
PF01139. RtcB. 2 hits.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF103365. SSF103365. 2 hits.
SSF51294. SSF51294. 3 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCB_PYRFU
AccessionPrimary (citable) accession number: Q8U0H4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries