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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain 2 (trpB2), Tryptophan synthase beta chain 1 (trpB1)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36Proton acceptor1
Active sitei47Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BRENDAi4.2.1.20. 5243.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotation
Ordered Locus Names:PF1705
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000988971 – 248Tryptophan synthase alpha chainAdd BLAST248

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.UniRule annotation

Protein-protein interaction databases

STRINGi186497.PF1705.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Helixi18 – 28Combined sources11
Helixi29 – 31Combined sources3
Beta strandi35 – 38Combined sources4
Helixi49 – 60Combined sources12
Helixi65 – 76Combined sources12
Beta strandi83 – 87Combined sources5
Helixi89 – 95Combined sources7
Helixi97 – 107Combined sources11
Beta strandi111 – 114Combined sources4
Helixi119 – 121Combined sources3
Helixi122 – 132Combined sources11
Beta strandi135 – 140Combined sources6
Helixi146 – 155Combined sources10
Beta strandi157 – 163Combined sources7
Helixi177 – 189Combined sources13
Beta strandi194 – 198Combined sources5
Helixi203 – 211Combined sources9
Beta strandi215 – 219Combined sources5
Helixi221 – 230Combined sources10
Helixi231 – 233Combined sources3
Helixi235 – 246Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GEQX-ray2.00A/B1-248[»]
1WDWX-ray3.00A/C/E/G/I/K1-248[»]
2DZPX-ray2.40A/B1-248[»]
2DZSX-ray2.40A/B1-248[»]
2DZTX-ray2.40A/B1-248[»]
2DZUX-ray2.46A/B1-248[»]
2DZVX-ray2.40A/B1-248[»]
2DZWX-ray2.40A/B1-248[»]
2DZXX-ray2.40A/B1-248[»]
2E09X-ray2.40A/B1-248[»]
5E0KX-ray2.76A/C/E/G/I/K1-248[»]
ProteinModelPortaliQ8U094.
SMRiQ8U094.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U094.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01086. Archaea.
COG0159. LUCA.
HOGENOMiHOG000223815.
KOiK01695.
OMAiPVFICPP.

Family and domain databases

CDDicd04724. Tryptophan_synthase_alpha. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U094-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKDGSLIPY LTAGDPDKQS TLNFLLALDE YAGAIELGIP FSDPIADGKT
60 70 80 90 100
IQESHYRALK NGFKLREAFW IVKEFRRHSS TPIVLMTYYN PIYRAGVRNF
110 120 130 140 150
LAEAKASGVD GILVVDLPVF HAKEFTEIAR EEGIKTVFLA APNTPDERLK
160 170 180 190 200
VIDDMTTGFV YLVSLYGTTG AREEIPKTAY DLLRRAKRIC RNKVAVGFGV
210 220 230 240
SKREHVVSLL KEGANGVVVG SALVKIIGEK GREATEFLKK KVEELLGI
Length:248
Mass (Da):27,497
Last modified:June 1, 2002 - v1
Checksum:i45D6C877FB8393EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080770 Genomic DNA. Translation: BAC11856.1.
AE009950 Genomic DNA. Translation: AAL81829.1.
RefSeqiWP_011012851.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81829; AAL81829; PF1705.
GeneIDi1469582.
KEGGipfu:PF1705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080770 Genomic DNA. Translation: BAC11856.1.
AE009950 Genomic DNA. Translation: AAL81829.1.
RefSeqiWP_011012851.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GEQX-ray2.00A/B1-248[»]
1WDWX-ray3.00A/C/E/G/I/K1-248[»]
2DZPX-ray2.40A/B1-248[»]
2DZSX-ray2.40A/B1-248[»]
2DZTX-ray2.40A/B1-248[»]
2DZUX-ray2.46A/B1-248[»]
2DZVX-ray2.40A/B1-248[»]
2DZWX-ray2.40A/B1-248[»]
2DZXX-ray2.40A/B1-248[»]
2E09X-ray2.40A/B1-248[»]
5E0KX-ray2.76A/C/E/G/I/K1-248[»]
ProteinModelPortaliQ8U094.
SMRiQ8U094.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81829; AAL81829; PF1705.
GeneIDi1469582.
KEGGipfu:PF1705.

Phylogenomic databases

eggNOGiarCOG01086. Archaea.
COG0159. LUCA.
HOGENOMiHOG000223815.
KOiK01695.
OMAiPVFICPP.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BRENDAi4.2.1.20. 5243.

Miscellaneous databases

EvolutionaryTraceiQ8U094.

Family and domain databases

CDDicd04724. Tryptophan_synthase_alpha. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA_PYRFU
AccessioniPrimary (citable) accession number: Q8U094
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.