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Q8U094

- TRPA_PYRFU

UniProt

Q8U094 - TRPA_PYRFU

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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361Proton acceptor
Active sitei47 – 471Proton acceptor

GO - Molecular functioni

  1. tryptophan synthase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotation
Ordered Locus Names:PF1705
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Tryptophan synthase alpha chainPRO_0000098897Add
BLAST

Proteomic databases

PRIDEiQ8U094.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.UniRule annotation

Protein-protein interaction databases

STRINGi186497.PF1705.

Structurei

Secondary structure

1
248
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127
Helixi18 – 2811
Helixi29 – 313
Beta strandi35 – 384
Helixi49 – 6012
Helixi65 – 7612
Beta strandi83 – 875
Helixi89 – 957
Helixi97 – 10711
Beta strandi111 – 1144
Helixi119 – 1213
Helixi122 – 13211
Beta strandi135 – 1406
Helixi146 – 15510
Beta strandi157 – 1637
Helixi177 – 18913
Beta strandi194 – 1985
Helixi203 – 2119
Beta strandi215 – 2195
Helixi221 – 23010
Helixi231 – 2333
Helixi235 – 24612

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GEQX-ray2.00A/B1-248[»]
1WDWX-ray3.00A/C/E/G/I/K1-248[»]
2DZPX-ray2.40A/B1-248[»]
2DZSX-ray2.40A/B1-248[»]
2DZTX-ray2.40A/B1-248[»]
2DZUX-ray2.46A/B1-248[»]
2DZVX-ray2.40A/B1-248[»]
2DZWX-ray2.40A/B1-248[»]
2DZXX-ray2.40A/B1-248[»]
2E09X-ray2.40A/B1-248[»]
ProteinModelPortaliQ8U094.
SMRiQ8U094. Positions 1-248.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U094.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0159.
HOGENOMiHOG000223815.
KOiK01695.
OMAiRIDRRMA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U094 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKDGSLIPY LTAGDPDKQS TLNFLLALDE YAGAIELGIP FSDPIADGKT
60 70 80 90 100
IQESHYRALK NGFKLREAFW IVKEFRRHSS TPIVLMTYYN PIYRAGVRNF
110 120 130 140 150
LAEAKASGVD GILVVDLPVF HAKEFTEIAR EEGIKTVFLA APNTPDERLK
160 170 180 190 200
VIDDMTTGFV YLVSLYGTTG AREEIPKTAY DLLRRAKRIC RNKVAVGFGV
210 220 230 240
SKREHVVSLL KEGANGVVVG SALVKIIGEK GREATEFLKK KVEELLGI
Length:248
Mass (Da):27,497
Last modified:June 1, 2002 - v1
Checksum:i45D6C877FB8393EE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB080770 Genomic DNA. Translation: BAC11856.1.
AE009950 Genomic DNA. Translation: AAL81829.1.
RefSeqiNP_579434.1. NC_003413.1.
WP_011012851.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81829; AAL81829; PF1705.
GeneIDi1469582.
KEGGipfu:PF1705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB080770 Genomic DNA. Translation: BAC11856.1 .
AE009950 Genomic DNA. Translation: AAL81829.1 .
RefSeqi NP_579434.1. NC_003413.1.
WP_011012851.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GEQ X-ray 2.00 A/B 1-248 [» ]
1WDW X-ray 3.00 A/C/E/G/I/K 1-248 [» ]
2DZP X-ray 2.40 A/B 1-248 [» ]
2DZS X-ray 2.40 A/B 1-248 [» ]
2DZT X-ray 2.40 A/B 1-248 [» ]
2DZU X-ray 2.46 A/B 1-248 [» ]
2DZV X-ray 2.40 A/B 1-248 [» ]
2DZW X-ray 2.40 A/B 1-248 [» ]
2DZX X-ray 2.40 A/B 1-248 [» ]
2E09 X-ray 2.40 A/B 1-248 [» ]
ProteinModelPortali Q8U094.
SMRi Q8U094. Positions 1-248.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186497.PF1705.

Proteomic databases

PRIDEi Q8U094.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL81829 ; AAL81829 ; PF1705 .
GeneIDi 1469582.
KEGGi pfu:PF1705.

Phylogenomic databases

eggNOGi COG0159.
HOGENOMi HOG000223815.
KOi K01695.
OMAi RIDRRMA.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00044 .

Miscellaneous databases

EvolutionaryTracei Q8U094.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00131. Trp_synth_alpha.
InterProi IPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view ]
Pfami PF00290. Trp_syntA. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR00262. trpA. 1 hit.
PROSITEi PS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus."
    Ishida M., Oshima T., Yutani K.
    FEMS Microbiol. Lett. 216:179-183(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  3. "Structures of mutant tryptophan synthase alpha-subunits from a hyperthermophile, Pyrococcus furiosus."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2007) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Entry informationi

Entry nameiTRPA_PYRFU
AccessioniPrimary (citable) accession number: Q8U094
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: June 1, 2002
Last modified: October 1, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3