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Q8U094

- TRPA_PYRFU

UniProt

Q8U094 - TRPA_PYRFU

Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.

    Catalytic activityi

    L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei36 – 361Proton acceptor
    Active sitei47 – 471Proton acceptor

    GO - Molecular functioni

    1. tryptophan synthase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00035; UER00044.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
    Gene namesi
    Name:trpAUniRule annotation
    Ordered Locus Names:PF1705
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 248248Tryptophan synthase alpha chainPRO_0000098897Add
    BLAST

    Proteomic databases

    PRIDEiQ8U094.

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta chains.UniRule annotation

    Protein-protein interaction databases

    STRINGi186497.PF1705.

    Structurei

    Secondary structure

    1
    248
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 127
    Helixi18 – 2811
    Helixi29 – 313
    Beta strandi35 – 384
    Helixi49 – 6012
    Helixi65 – 7612
    Beta strandi83 – 875
    Helixi89 – 957
    Helixi97 – 10711
    Beta strandi111 – 1144
    Helixi119 – 1213
    Helixi122 – 13211
    Beta strandi135 – 1406
    Helixi146 – 15510
    Beta strandi157 – 1637
    Helixi177 – 18913
    Beta strandi194 – 1985
    Helixi203 – 2119
    Beta strandi215 – 2195
    Helixi221 – 23010
    Helixi231 – 2333
    Helixi235 – 24612

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GEQX-ray2.00A/B1-248[»]
    1WDWX-ray3.00A/C/E/G/I/K1-248[»]
    2DZPX-ray2.40A/B1-248[»]
    2DZSX-ray2.40A/B1-248[»]
    2DZTX-ray2.40A/B1-248[»]
    2DZUX-ray2.46A/B1-248[»]
    2DZVX-ray2.40A/B1-248[»]
    2DZWX-ray2.40A/B1-248[»]
    2DZXX-ray2.40A/B1-248[»]
    2E09X-ray2.40A/B1-248[»]
    ProteinModelPortaliQ8U094.
    SMRiQ8U094. Positions 1-248.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8U094.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TrpA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0159.
    HOGENOMiHOG000223815.
    KOiK01695.
    OMAiRIDRRMA.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00131. Trp_synth_alpha.
    InterProiIPR013785. Aldolase_TIM.
    IPR011060. RibuloseP-bd_barrel.
    IPR018204. Trp_synthase_alpha_AS.
    IPR002028. Trp_synthase_suA.
    [Graphical view]
    PfamiPF00290. Trp_syntA. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    TIGRFAMsiTIGR00262. trpA. 1 hit.
    PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8U094-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKDGSLIPY LTAGDPDKQS TLNFLLALDE YAGAIELGIP FSDPIADGKT    50
    IQESHYRALK NGFKLREAFW IVKEFRRHSS TPIVLMTYYN PIYRAGVRNF 100
    LAEAKASGVD GILVVDLPVF HAKEFTEIAR EEGIKTVFLA APNTPDERLK 150
    VIDDMTTGFV YLVSLYGTTG AREEIPKTAY DLLRRAKRIC RNKVAVGFGV 200
    SKREHVVSLL KEGANGVVVG SALVKIIGEK GREATEFLKK KVEELLGI 248
    Length:248
    Mass (Da):27,497
    Last modified:June 1, 2002 - v1
    Checksum:i45D6C877FB8393EE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB080770 Genomic DNA. Translation: BAC11856.1.
    AE009950 Genomic DNA. Translation: AAL81829.1.
    RefSeqiNP_579434.1. NC_003413.1.
    WP_011012851.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81829; AAL81829; PF1705.
    GeneIDi1469582.
    KEGGipfu:PF1705.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB080770 Genomic DNA. Translation: BAC11856.1 .
    AE009950 Genomic DNA. Translation: AAL81829.1 .
    RefSeqi NP_579434.1. NC_003413.1.
    WP_011012851.1. NC_003413.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GEQ X-ray 2.00 A/B 1-248 [» ]
    1WDW X-ray 3.00 A/C/E/G/I/K 1-248 [» ]
    2DZP X-ray 2.40 A/B 1-248 [» ]
    2DZS X-ray 2.40 A/B 1-248 [» ]
    2DZT X-ray 2.40 A/B 1-248 [» ]
    2DZU X-ray 2.46 A/B 1-248 [» ]
    2DZV X-ray 2.40 A/B 1-248 [» ]
    2DZW X-ray 2.40 A/B 1-248 [» ]
    2DZX X-ray 2.40 A/B 1-248 [» ]
    2E09 X-ray 2.40 A/B 1-248 [» ]
    ProteinModelPortali Q8U094.
    SMRi Q8U094. Positions 1-248.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 186497.PF1705.

    Proteomic databases

    PRIDEi Q8U094.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL81829 ; AAL81829 ; PF1705 .
    GeneIDi 1469582.
    KEGGi pfu:PF1705.

    Phylogenomic databases

    eggNOGi COG0159.
    HOGENOMi HOG000223815.
    KOi K01695.
    OMAi RIDRRMA.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00044 .

    Miscellaneous databases

    EvolutionaryTracei Q8U094.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00131. Trp_synth_alpha.
    InterProi IPR013785. Aldolase_TIM.
    IPR011060. RibuloseP-bd_barrel.
    IPR018204. Trp_synthase_alpha_AS.
    IPR002028. Trp_synthase_suA.
    [Graphical view ]
    Pfami PF00290. Trp_syntA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    TIGRFAMsi TIGR00262. trpA. 1 hit.
    PROSITEi PS00167. TRP_SYNTHASE_ALPHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus."
      Ishida M., Oshima T., Yutani K.
      FEMS Microbiol. Lett. 216:179-183(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    2. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    3. "Structures of mutant tryptophan synthase alpha-subunits from a hyperthermophile, Pyrococcus furiosus."
      RIKEN structural genomics initiative (RSGI)
      Submitted (OCT-2007) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

    Entry informationi

    Entry nameiTRPA_PYRFU
    AccessioniPrimary (citable) accession number: Q8U094
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3