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Protein

Tryptophan synthase beta chain 1

Gene

trpB1

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.By similarity

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain 2 (trpB2), Tryptophan synthase beta chain 1 (trpB1)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi4.2.1.20. 5243.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain 1 (EC:4.2.1.20)
Gene namesi
Name:trpB1
Ordered Locus Names:PF1706
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000990501 – 388Tryptophan synthase beta chain 1Add BLAST388

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei82N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PRIDEiQ8U093.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.By similarity

Protein-protein interaction databases

STRINGi186497.PF1706.

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 15Combined sources3
Helixi16 – 29Combined sources14
Helixi33 – 45Combined sources13
Beta strandi53 – 55Combined sources3
Helixi57 – 63Combined sources7
Beta strandi65 – 71Combined sources7
Helixi73 – 75Combined sources3
Helixi82 – 95Combined sources14
Beta strandi100 – 108Combined sources9
Helixi109 – 121Combined sources13
Beta strandi124 – 130Combined sources7
Helixi131 – 136Combined sources6
Helixi138 – 146Combined sources9
Beta strandi150 – 154Combined sources5
Helixi161 – 175Combined sources15
Turni176 – 178Combined sources3
Beta strandi179 – 181Combined sources3
Beta strandi184 – 186Combined sources3
Helixi192 – 215Combined sources24
Beta strandi220 – 225Combined sources6
Beta strandi227 – 229Combined sources3
Helixi230 – 236Combined sources7
Helixi237 – 239Combined sources3
Beta strandi246 – 254Combined sources9
Helixi257 – 259Combined sources3
Helixi265 – 268Combined sources4
Beta strandi270 – 274Combined sources5
Beta strandi277 – 281Combined sources5
Beta strandi287 – 289Combined sources3
Helixi297 – 299Combined sources3
Helixi306 – 313Combined sources8
Beta strandi316 – 323Combined sources8
Helixi324 – 338Combined sources15
Helixi344 – 359Combined sources16
Beta strandi365 – 369Combined sources5
Beta strandi372 – 374Combined sources3
Helixi375 – 377Combined sources3
Helixi378 – 383Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V8ZX-ray2.21A/B/C/D1-388[»]
1WDWX-ray3.00B/D/F/H/J/L1-385[»]
5DVZX-ray1.69A/B/C/D1-388[»]
5DW0X-ray2.01A/B/C/D1-388[»]
5DW3X-ray1.74A/B/C/D1-388[»]
5E0KX-ray2.76B/D/F/H/J/L1-388[»]
5IXJX-ray1.54A/B/C/D1-388[»]
ProteinModelPortaliQ8U093.
SMRiQ8U093.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U093.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Phylogenomic databases

eggNOGiarCOG01433. Archaea.
COG0133. LUCA.
HOGENOMiHOG000161710.
KOiK01696.
OMAiCQKEGII.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFGEFGGQY VPETLIEPLK ELEKAYKRFK DDEEFNRQLN YYLKTWAGRP
60 70 80 90 100
TPLYYAKRLT EKIGGAKIYL KREDLVHGGA HKTNNAIGQA LLAKFMGKTR
110 120 130 140 150
LIAETGAGQH GVATAMAGAL LGMKVDIYMG AEDVERQKMN VFRMKLLGAN
160 170 180 190 200
VIPVNSGSRT LKDAINEALR DWVATFEYTH YLIGSVVGPH PYPTIVRDFQ
210 220 230 240 250
SVIGREAKAQ ILEAEGQLPD VIVACVGGGS NAMGIFYPFV NDKKVKLVGV
260 270 280 290 300
EAGGKGLESG KHSASLNAGQ VGVFHGMLSY FLQDEEGQIK PTHSIAPGLD
310 320 330 340 350
YPGVGPEHAY LKKIQRAEYV TVTDEEALKA FHELSRTEGI IPALESAHAV
360 370 380
AYAMKLAKEM SRDEIIIVNL SGRGDKDLDI VLKVSGNV
Length:388
Mass (Da):42,529
Last modified:June 1, 2002 - v1
Checksum:i62C811579520E04B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080770 Genomic DNA. Translation: BAC11855.1.
AE009950 Genomic DNA. Translation: AAL81830.1.
RefSeqiWP_011012852.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81830; AAL81830; PF1706.
GeneIDi1469583.
KEGGipfu:PF1706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080770 Genomic DNA. Translation: BAC11855.1.
AE009950 Genomic DNA. Translation: AAL81830.1.
RefSeqiWP_011012852.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V8ZX-ray2.21A/B/C/D1-388[»]
1WDWX-ray3.00B/D/F/H/J/L1-385[»]
5DVZX-ray1.69A/B/C/D1-388[»]
5DW0X-ray2.01A/B/C/D1-388[»]
5DW3X-ray1.74A/B/C/D1-388[»]
5E0KX-ray2.76B/D/F/H/J/L1-388[»]
5IXJX-ray1.54A/B/C/D1-388[»]
ProteinModelPortaliQ8U093.
SMRiQ8U093.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1706.

Proteomic databases

PRIDEiQ8U093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81830; AAL81830; PF1706.
GeneIDi1469583.
KEGGipfu:PF1706.

Phylogenomic databases

eggNOGiarCOG01433. Archaea.
COG0133. LUCA.
HOGENOMiHOG000161710.
KOiK01696.
OMAiCQKEGII.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BRENDAi4.2.1.20. 5243.

Miscellaneous databases

EvolutionaryTraceiQ8U093.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPB1_PYRFU
AccessioniPrimary (citable) accession number: Q8U093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.