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Protein

Tryptophan synthase beta chain 1

Gene

trpB1

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.By similarity

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi

GO - Molecular functioni

  1. tryptophan synthase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi4.2.1.20. 5243.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain 1 (EC:4.2.1.20)
Gene namesi
Name:trpB1
Ordered Locus Names:PF1706
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Tryptophan synthase beta chain 1PRO_0000099050Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei82 – 821N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PRIDEiQ8U093.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.By similarity

Protein-protein interaction databases

STRINGi186497.PF1706.

Structurei

Secondary structure

1
388
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 153Combined sources
Helixi16 – 3015Combined sources
Helixi33 – 4513Combined sources
Beta strandi53 – 553Combined sources
Helixi57 – 637Combined sources
Beta strandi65 – 728Combined sources
Helixi73 – 753Combined sources
Helixi82 – 9514Combined sources
Beta strandi100 – 1089Combined sources
Helixi109 – 12012Combined sources
Beta strandi124 – 1307Combined sources
Helixi131 – 1344Combined sources
Helixi138 – 1469Combined sources
Beta strandi150 – 1545Combined sources
Helixi161 – 17515Combined sources
Turni176 – 1783Combined sources
Beta strandi179 – 1813Combined sources
Helixi192 – 21524Combined sources
Beta strandi220 – 2256Combined sources
Beta strandi227 – 2293Combined sources
Helixi230 – 2367Combined sources
Helixi237 – 2393Combined sources
Beta strandi245 – 25410Combined sources
Helixi257 – 2593Combined sources
Helixi265 – 2684Combined sources
Beta strandi270 – 2745Combined sources
Beta strandi277 – 2815Combined sources
Helixi297 – 2993Combined sources
Helixi306 – 3138Combined sources
Beta strandi316 – 3238Combined sources
Helixi324 – 33815Combined sources
Helixi344 – 35815Combined sources
Beta strandi365 – 3706Combined sources
Beta strandi372 – 3743Combined sources
Helixi375 – 3773Combined sources
Helixi378 – 3858Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V8ZX-ray2.21A/B/C/D1-388[»]
1WDWX-ray3.00B/D/F/H/J/L1-385[»]
ProteinModelPortaliQ8U093.
SMRiQ8U093. Positions 1-387.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U093.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Phylogenomic databases

eggNOGiCOG0133.
HOGENOMiHOG000161710.
KOiK01696.
OMAiEYVGITD.

Family and domain databases

HAMAPiMF_00133. Trp_synth_beta.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFGEFGGQY VPETLIEPLK ELEKAYKRFK DDEEFNRQLN YYLKTWAGRP
60 70 80 90 100
TPLYYAKRLT EKIGGAKIYL KREDLVHGGA HKTNNAIGQA LLAKFMGKTR
110 120 130 140 150
LIAETGAGQH GVATAMAGAL LGMKVDIYMG AEDVERQKMN VFRMKLLGAN
160 170 180 190 200
VIPVNSGSRT LKDAINEALR DWVATFEYTH YLIGSVVGPH PYPTIVRDFQ
210 220 230 240 250
SVIGREAKAQ ILEAEGQLPD VIVACVGGGS NAMGIFYPFV NDKKVKLVGV
260 270 280 290 300
EAGGKGLESG KHSASLNAGQ VGVFHGMLSY FLQDEEGQIK PTHSIAPGLD
310 320 330 340 350
YPGVGPEHAY LKKIQRAEYV TVTDEEALKA FHELSRTEGI IPALESAHAV
360 370 380
AYAMKLAKEM SRDEIIIVNL SGRGDKDLDI VLKVSGNV
Length:388
Mass (Da):42,529
Last modified:May 31, 2002 - v1
Checksum:i62C811579520E04B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080770 Genomic DNA. Translation: BAC11855.1.
AE009950 Genomic DNA. Translation: AAL81830.1.
RefSeqiNP_579435.1. NC_003413.1.
WP_011012852.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81830; AAL81830; PF1706.
GeneIDi1469583.
KEGGipfu:PF1706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080770 Genomic DNA. Translation: BAC11855.1.
AE009950 Genomic DNA. Translation: AAL81830.1.
RefSeqiNP_579435.1. NC_003413.1.
WP_011012852.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V8ZX-ray2.21A/B/C/D1-388[»]
1WDWX-ray3.00B/D/F/H/J/L1-385[»]
ProteinModelPortaliQ8U093.
SMRiQ8U093. Positions 1-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1706.

Proteomic databases

PRIDEiQ8U093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81830; AAL81830; PF1706.
GeneIDi1469583.
KEGGipfu:PF1706.

Phylogenomic databases

eggNOGiCOG0133.
HOGENOMiHOG000161710.
KOiK01696.
OMAiEYVGITD.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BRENDAi4.2.1.20. 5243.

Miscellaneous databases

EvolutionaryTraceiQ8U093.

Family and domain databases

HAMAPiMF_00133. Trp_synth_beta.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus."
    Ishida M., Oshima T., Yutani K.
    FEMS Microbiol. Lett. 216:179-183(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiTRPB1_PYRFU
AccessioniPrimary (citable) accession number: Q8U093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2002
Last sequence update: May 31, 2002
Last modified: March 31, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.