Q8U092 (TRPF_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-(5'-phosphoribosyl)anthranilate isomerase
Short name=PRAI
EC=5.3.1.24

Gene names

Name:	trpF
Ordered Locus Names:	PF1707

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus furiosus

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP-Rule MF_00135
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5. HAMAP-Rule MF_00135
Sequence similarities	Belongs to the TrpF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	phosphoribosylanthranilate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 208

208

N-(5'-phosphoribosyl)anthranilate isomerase HAMAP-Rule MF_00135

PRO_0000154412

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

208

Helix Strand Turn

Details...

Beta strand

2 – 5

4

Helix

11 – 18

8

Beta strand

22 – 27

6

Beta strand

29 – 32

4

Helix

38 – 47

10

Beta strand

52 – 56

5

Helix

61 – 71

11

Beta strand

74 – 78

5

Helix

84 – 94

11

Beta strand

97 – 103

7

Helix

111 – 124

14

Beta strand

128 – 133

6

Helix

145 – 153

9

Beta strand

156 – 161

6

Turn

164 – 166

3

Helix

167 – 174

8

Beta strand

177 – 182

6

Helix

183 – 185

3

Helix

193 – 205

13

Sequences

	Sequence		Length	Mass (Da)
	Q8U092 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2A00EE4E9FDB7495	FASTA	208	22,994
	10 20 30 40 50 60 MFVKICGIKS LEELEIVEKH ADATGVVVNS NSKRRIPLEK AREIIENSAI PVFLVSTMVG 70 80 90 100 110 120 FSEWAMAIER TGAQYIQVHS NALPQTIDTL KKEFGVFVMK AFRVPTISKN PEEDANRLLS 130 140 150 160 170 180 EISRYNADMV LLDTGAGSGK LHDLRVSSLV ARKIPVIVAG GLNAENVEEV IKVVKPYGVD 190 200 VSSGVEKYGI KDPKLVEEFV RRAKNVVW

References

[1]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE009950 Genomic DNA. Translation: AAL81831.1.

RefSeq

NP_579436.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4AAJ	X-ray	1.75	A	1-208	[»]

ProteinModelPortal

Q8U092.

ModBase

Search...

Protein-protein interaction databases

STRING

186497.PF1707.

Proteomic databases

PRIDE

Q8U092.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL81831; AAL81831; PF1707.

GeneID

1469584.

KEGG

pfu:PF1707.

Phylogenomic databases

eggNOG

COG0135.

HOGENOM

HOG000161598.

KO

K01817.

OMA

TGVILEC.

ProtClustDB

PRK01222.

Enzyme and pathway databases

UniPathway

UPA00035; UER00042.

Family and domain databases

Gene3D

3.20.20.70. 1 hit.

HAMAP

MF_00135. PRAI.

InterPro

IPR013785. Aldolase_TIM.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]

Pfam

PF00697. PRAI. 1 hit.
[Graphical view]

SUPFAM

SSF51366. RibP_bind_barrel. 1 hit.

ProtoNet

Search...

Entry information

Entry name

TRPF_PYRFU

Accession

Primary (citable) accession number: Q8U092

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program