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Protein

Signal recognition particle 54 kDa protein

Gene

srp54

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 114GTPUniRule annotation8
Nucleotide bindingi189 – 193GTPUniRule annotation5
Nucleotide bindingi247 – 250GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.4 5243

Protein family/group databases

TCDBi3.A.5.7.2 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa proteinUniRule annotation
Short name:
SRP54UniRule annotation
Gene namesi
Name:srp54UniRule annotation
Ordered Locus Names:PF1731
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011831 – 443Signal recognition particle 54 kDa proteinAdd BLAST443

Proteomic databases

PRIDEiQ8U070

Interactioni

Subunit structurei

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19 (Probable).Curated

Protein-protein interaction databases

STRINGi186497.PF1731

Structurei

Secondary structure

1443
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 19Combined sources15
Helixi25 – 41Combined sources17
Helixi46 – 62Combined sources17
Helixi71 – 86Combined sources16
Beta strandi98 – 106Combined sources9
Helixi113 – 125Combined sources13
Turni126 – 128Combined sources3
Beta strandi131 – 135Combined sources5
Helixi142 – 151Combined sources10
Helixi152 – 154Combined sources3
Beta strandi157 – 159Combined sources3
Helixi167 – 180Combined sources14
Beta strandi184 – 189Combined sources6
Helixi198 – 211Combined sources14
Beta strandi214 – 221Combined sources8
Helixi222 – 227Combined sources6
Helixi228 – 237Combined sources10
Beta strandi242 – 247Combined sources6
Helixi255 – 263Combined sources9
Beta strandi269 – 273Combined sources5
Beta strandi275 – 277Combined sources3
Beta strandi281 – 283Combined sources3
Helixi286 – 293Combined sources8
Turni294 – 297Combined sources4
Helixi299 – 310Combined sources12
Helixi313 – 324Combined sources12
Helixi330 – 341Combined sources12
Helixi369 – 379Combined sources11
Helixi384 – 388Combined sources5
Helixi390 – 392Combined sources3
Helixi395 – 405Combined sources11
Helixi409 – 427Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DM5X-ray2.51A/B1-443[»]
ProteinModelPortaliQ8U070
SMRiQ8U070
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U070

Family & Domainsi

Domaini

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01228 Archaea
COG0541 LUCA
HOGENOMiHOG000036165
KOiK03106
OMAiDTAGRHK
OrthoDBiPOG093Z02NX

Family and domain databases

Gene3Di1.10.260.30, 1 hit
HAMAPiMF_00306 SRP54, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR036891 Signal_recog_part_SRP54_M_sf
IPR013822 Signal_recog_particl_SRP54_hlx
IPR004125 Signal_recog_particle_SRP54_M
IPR036225 SRP/SRP_N
IPR022941 SRP54
IPR000897 SRP54_GTPase_dom
PANTHERiPTHR11564 PTHR11564, 1 hit
PfamiView protein in Pfam
PF00448 SRP54, 1 hit
PF02881 SRP54_N, 1 hit
PF02978 SRP_SPB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00962 SRP54, 1 hit
SM00963 SRP54_N, 1 hit
SUPFAMiSSF47364 SSF47364, 1 hit
SSF47446 SSF47446, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00300 SRP54, 1 hit

Sequencei

Sequence statusi: Complete.

Q8U070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLDNLGKAL ANTLKKIARA SSVDEALIKE LVRDIQRALI QADVNVRLVL
60 70 80 90 100
QLTREIQRRA LEEKPPAGIS KKEHIIKIVY EELTKFLGTE AKPIEIKEKP
110 120 130 140 150
TILLMVGIQG SGKTTTVAKL ARYFQKRGYK VGVVCSDTWR PGAYHQLRQL
160 170 180 190 200
LDRYHIEVFG NPQEKDAIKL AKEGVDYFKS KGVDIIIVDT AGRHKEDKAL
210 220 230 240 250
IEEMKQISNV IHPHEVILVI DGTIGQQAYN QALAFKEATP IGSIIVTKLD
260 270 280 290 300
GSAKGGGALS AVAATGAPIK FIGTGEKIDD IEPFDPPRFV SRLLGLGDIQ
310 320 330 340 350
GLLEKFKELE KEVEIKEEDI ERFLRGKFTL KDMYAQLEAM RKMGPLKQIL
360 370 380 390 400
RMIPGLGYSL PDDVISIGEE RLKKFKVIMD SMTEEELLNP EIINYSRIKR
410 420 430 440
IARGSGTSTK DVKELLDQYR QMKKLFKSMN KRQLSRLARR FGM
Length:443
Mass (Da):49,879
Last modified:June 1, 2002 - v1
Checksum:i102D1717B207F71A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150L → V in BAB64926 (PubMed:11696367).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057373 Genomic DNA Translation: BAB64926.1
AE009950 Genomic DNA Translation: AAL81855.1
RefSeqiWP_011012877.1, NC_003413.1

Genome annotation databases

EnsemblBacteriaiAAL81855; AAL81855; PF1731
GeneIDi1469608
KEGGipfu:PF1731
PATRICifig|186497.12.peg.1799

Similar proteinsi

Entry informationi

Entry nameiSRP54_PYRFU
AccessioniPrimary (citable) accession number: Q8U070
Secondary accession number(s): Q977Q0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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