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Protein

Signal recognition particle 54 kDa protein

Gene

srp54

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi107 – 1148GTPUniRule annotation
Nucleotide bindingi189 – 1935GTPUniRule annotation
Nucleotide bindingi247 – 2504GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BRENDAi3.6.5.4. 5243.

Protein family/group databases

TCDBi3.A.5.7.2. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa proteinUniRule annotation
Short name:
SRP54UniRule annotation
Gene namesi
Name:srp54UniRule annotation
Ordered Locus Names:PF1731
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

  • Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Signal recognition particle 54 kDa proteinPRO_0000101183Add
BLAST

Proteomic databases

PRIDEiQ8U070.

Interactioni

Subunit structurei

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19 (Probable).Curated

Protein-protein interaction databases

STRINGi186497.PF1731.

Structurei

Secondary structure

1
443
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1915Combined sources
Helixi25 – 4117Combined sources
Helixi46 – 6217Combined sources
Helixi71 – 8616Combined sources
Beta strandi98 – 1069Combined sources
Helixi113 – 12513Combined sources
Turni126 – 1283Combined sources
Beta strandi131 – 1355Combined sources
Helixi142 – 15110Combined sources
Helixi152 – 1543Combined sources
Beta strandi157 – 1593Combined sources
Helixi167 – 18014Combined sources
Beta strandi184 – 1896Combined sources
Helixi198 – 21114Combined sources
Beta strandi214 – 2218Combined sources
Helixi222 – 2276Combined sources
Helixi228 – 23710Combined sources
Beta strandi242 – 2476Combined sources
Helixi255 – 2639Combined sources
Beta strandi269 – 2735Combined sources
Beta strandi275 – 2773Combined sources
Beta strandi281 – 2833Combined sources
Helixi286 – 2938Combined sources
Turni294 – 2974Combined sources
Helixi299 – 31012Combined sources
Helixi313 – 32412Combined sources
Helixi330 – 34112Combined sources
Helixi369 – 37911Combined sources
Helixi384 – 3885Combined sources
Helixi390 – 3923Combined sources
Helixi395 – 40511Combined sources
Helixi409 – 42719Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DM5X-ray2.51A/B1-443[»]
ProteinModelPortaliQ8U070.
SMRiQ8U070. Positions 3-429.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U070.

Family & Domainsi

Domaini

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01228. Archaea.
COG0541. LUCA.
HOGENOMiHOG000036165.
KOiK03106.
OMAiMEKMMSG.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLDNLGKAL ANTLKKIARA SSVDEALIKE LVRDIQRALI QADVNVRLVL
60 70 80 90 100
QLTREIQRRA LEEKPPAGIS KKEHIIKIVY EELTKFLGTE AKPIEIKEKP
110 120 130 140 150
TILLMVGIQG SGKTTTVAKL ARYFQKRGYK VGVVCSDTWR PGAYHQLRQL
160 170 180 190 200
LDRYHIEVFG NPQEKDAIKL AKEGVDYFKS KGVDIIIVDT AGRHKEDKAL
210 220 230 240 250
IEEMKQISNV IHPHEVILVI DGTIGQQAYN QALAFKEATP IGSIIVTKLD
260 270 280 290 300
GSAKGGGALS AVAATGAPIK FIGTGEKIDD IEPFDPPRFV SRLLGLGDIQ
310 320 330 340 350
GLLEKFKELE KEVEIKEEDI ERFLRGKFTL KDMYAQLEAM RKMGPLKQIL
360 370 380 390 400
RMIPGLGYSL PDDVISIGEE RLKKFKVIMD SMTEEELLNP EIINYSRIKR
410 420 430 440
IARGSGTSTK DVKELLDQYR QMKKLFKSMN KRQLSRLARR FGM
Length:443
Mass (Da):49,879
Last modified:June 1, 2002 - v1
Checksum:i102D1717B207F71A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti150 – 1501L → V in BAB64926 (PubMed:11696367).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057373 Genomic DNA. Translation: BAB64926.1.
AE009950 Genomic DNA. Translation: AAL81855.1.
RefSeqiWP_011012877.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81855; AAL81855; PF1731.
GeneIDi1469608.
KEGGipfu:PF1731.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB057373 Genomic DNA. Translation: BAB64926.1.
AE009950 Genomic DNA. Translation: AAL81855.1.
RefSeqiWP_011012877.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DM5X-ray2.51A/B1-443[»]
ProteinModelPortaliQ8U070.
SMRiQ8U070. Positions 3-429.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1731.

Protein family/group databases

TCDBi3.A.5.7.2. the general secretory pathway (sec) family.

Proteomic databases

PRIDEiQ8U070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81855; AAL81855; PF1731.
GeneIDi1469608.
KEGGipfu:PF1731.

Phylogenomic databases

eggNOGiarCOG01228. Archaea.
COG0541. LUCA.
HOGENOMiHOG000036165.
KOiK03106.
OMAiMEKMMSG.

Enzyme and pathway databases

BRENDAi3.6.5.4. 5243.

Miscellaneous databases

EvolutionaryTraceiQ8U070.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRP54_PYRFU
AccessioniPrimary (citable) accession number: Q8U070
Secondary accession number(s): Q977Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.