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Protein

Protein translocase subunit SecY

Gene

secY

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. Complements an E.coli temperature-sensitive secY mutation; deletion of the last 15 residues prevents complementation, which may indicate a role of this region in translocation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei78 – 781Pore ring
Sitei82 – 821Pore ring
Sitei177 – 1771Pore ring
Sitei181 – 1811Pore ring
Sitei291 – 2911Pore ring
Sitei438 – 4381Pore ring

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Protein family/group databases

TCDBi3.A.5.7.2. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecY
Alternative name(s):
Protein transport protein SEC61 subunit alpha homolog
Gene namesi
Name:secY
Ordered Locus Names:PF1801
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicAdd
BLAST
Transmembranei34 – 4815Helical; Name=Helix 1Add
BLAST
Topological domaini49 – 579Extracellular
Transmembranei58 – 8831Discontinuously helical; Name=Helix 2Add
BLAST
Intramembranei58 – 7114Helical; Name=Helix 2AAdd
BLAST
Intramembranei72 – 721
Intramembranei73 – 8816Helical; Name=Helix 2BAdd
BLAST
Topological domaini89 – 11527CytoplasmicAdd
BLAST
Transmembranei116 – 13015Helical; Name=Helix 3Add
BLAST
Topological domaini131 – 14818ExtracellularAdd
BLAST
Transmembranei149 – 16618Helical; Name=Helix 4Add
BLAST
Topological domaini167 – 17610Cytoplasmic
Transmembranei177 – 19620Helical; Name=Helix 5Add
BLAST
Topological domaini197 – 23943ExtracellularAdd
BLAST
Transmembranei240 – 25718Helical; Name=Helix 6Add
BLAST
Topological domaini258 – 28528CytoplasmicAdd
BLAST
Transmembranei286 – 30318Helical; Name=Helix 7Add
BLAST
Topological domaini304 – 34643ExtracellularAdd
BLAST
Transmembranei347 – 36317Helical; Name=Helix 8Add
BLAST
Topological domaini364 – 40744CytoplasmicAdd
BLAST
Transmembranei408 – 42720Helical; Name=Helix 9Add
BLAST
Topological domaini428 – 4281Extracellular
Transmembranei429 – 44315Helical; Name=Helix 10Add
BLAST
Topological domaini444 – 46825CytoplasmicAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi454 – 46815Missing : No longer complements secY24, an E.coli temperature-sensitive secY mutation. 1 PublicationAdd
BLAST
Mutagenesisi459 – 4613FPA → PPP: No longer complements secY24, an E.coli temperature-sensitive secY mutation. 1 Publication
Mutagenesisi463 – 4642RK → AA: No longer complements secY24, an E.coli temperature-sensitive secY mutation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Protein translocase subunit SecYPRO_0000131768Add
BLAST

Proteomic databases

PRIDEiQ8U019.

Interactioni

Subunit structurei

Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved. The beta subunit (SecG) was lost during crystallization.1 Publication

Protein-protein interaction databases

DIPiDIP-59389N.
STRINGi186497.PF1801.

Structurei

Secondary structure

1
468
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni27 – 293Combined sources
Helixi30 – 4516Combined sources
Turni62 – 665Combined sources
Helixi79 – 879Combined sources
Helixi115 – 13117Combined sources
Beta strandi132 – 1365Combined sources
Helixi147 – 17125Combined sources
Beta strandi172 – 1743Combined sources
Helixi176 – 19318Combined sources
Beta strandi207 – 2093Combined sources
Helixi215 – 22511Combined sources
Beta strandi235 – 2384Combined sources
Helixi241 – 25919Combined sources
Beta strandi281 – 2844Combined sources
Helixi287 – 30721Combined sources
Beta strandi313 – 3153Combined sources
Helixi329 – 3313Combined sources
Beta strandi336 – 3383Combined sources
Helixi340 – 3434Combined sources
Helixi345 – 36925Combined sources
Helixi374 – 3763Combined sources
Turni390 – 3956Combined sources
Helixi409 – 42719Combined sources
Helixi434 – 45724Combined sources
Helixi459 – 4646Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MP7X-ray2.90A1-468[»]
ProteinModelPortaliQ8U019.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U019.

Family & Domainsi

Sequence similaritiesi

Belongs to the SecY/SEC61-alpha family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG04169. Archaea.
COG0201. LUCA.
HOGENOMiHOG000231248.
KOiK03076.
OMAiRYIPYVT.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
HAMAPiMF_01465. SecY. 1 hit.
InterProiIPR026593. SecY.
IPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]
PIRSFiPIRSF004557. SecY. 1 hit.
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8U019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGARDIIYAL ERWFPEVERP KRRVPLRERF MWTGVALILY YVLAEIPVYG
60 70 80 90 100
IPERIQDYFQ FLRVVLAGRN GSILTLGIGP IVTAGIILQL LVGSEIIKLD
110 120 130 140 150
LANPEDRRFY QALQRVFSVF MCFFEAAVWI LGGAFGRVGV DVTYAIAVLM
160 170 180 190 200
ILQLAMGGIV LIILDELVSK WGIGSGISLF IAAGVSQTIL TRSLNPLTDP
210 220 230 240 250
NIIDPLTGQP AIVGAIPYFI QHILKGDLWG AIYRGGSAPD MLSVVATIVV
260 270 280 290 300
FFIVVYFESM RVEIPLGYRG VTVRGSYPIR FLYVSNIPII LTFALYANIQ
310 320 330 340 350
LWARVLDRLG HPWLGRFDPT TGSPISGFVL YVIPPRNIFS VIDNPVRAIV
360 370 380 390 400
YLILTVIFSL LFGYLWVELT GLDARSIARQ LQRAGLQIPG FRRDPRTLEK
410 420 430 440 450
VLQRYIPYVT FWGSLTVALI AVLADFLGAL GTGTGILLTV GILYRFYEEI
460
AREQITEMFP ALRKLFGA
Length:468
Mass (Da):52,299
Last modified:June 1, 2002 - v1
Checksum:i7969DCB9C1983F68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81925.1.
RefSeqiWP_011012942.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81925; AAL81925; PF1801.
GeneIDi1469680.
KEGGipfu:PF1801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81925.1.
RefSeqiWP_011012942.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MP7X-ray2.90A1-468[»]
ProteinModelPortaliQ8U019.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59389N.
STRINGi186497.PF1801.

Protein family/group databases

TCDBi3.A.5.7.2. the general secretory pathway (sec) family.

Proteomic databases

PRIDEiQ8U019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81925; AAL81925; PF1801.
GeneIDi1469680.
KEGGipfu:PF1801.

Phylogenomic databases

eggNOGiarCOG04169. Archaea.
COG0201. LUCA.
HOGENOMiHOG000231248.
KOiK03076.
OMAiRYIPYVT.

Miscellaneous databases

EvolutionaryTraceiQ8U019.

Family and domain databases

Gene3Di1.10.3370.10. 1 hit.
HAMAPiMF_01465. SecY. 1 hit.
InterProiIPR026593. SecY.
IPR002208. SecY/SEC61-alpha.
IPR030659. SecY_CS.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]
PANTHERiPTHR10906. PTHR10906. 1 hit.
PfamiPF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]
PIRSFiPIRSF004557. SecY. 1 hit.
SUPFAMiSSF103491. SSF103491. 1 hit.
TIGRFAMsiTIGR00967. 3a0501s007. 1 hit.
PROSITEiPS00755. SECY_1. 1 hit.
PS00756. SECY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSECY_PYRFU
AccessioniPrimary (citable) accession number: Q8U019
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.