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Protein

Ribonuclease P protein component 1

Gene

rnp1

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. The RNA is catalytic, but its KM for pre-tRNA is 170-fold decreased in the presence of the 4 known protein subunits (Rnp1-4). The protein subunits also decrease the amount of Mg2+ needed for activity.UniRule annotation3 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation1 Publication

Kineticsi

kcat is 9.5 min(-1). For enzyme reconstituted with RNA and 4 known subunits (Rnp1-4).

  1. KM=0.18 µM for E.coli pre-tRNA(Tyr)1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein component 1UniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P component 1UniRule annotation
    Alternative name(s):
    Rpp29UniRule annotation
    Gene namesi
    Name:rnp1UniRule annotation
    Ordered Locus Names:PF1816
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    Proteomesi
    • UP000001013 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1 – 3131Missing : No interaction with Rnp4, does not reconstitute RNase P activity. 1 PublicationAdd
    BLAST
    Mutagenesisi1 – 2424Missing : No interaction with Rnp4, reconstitutes RNase P activity. 1 PublicationAdd
    BLAST
    Mutagenesisi1 – 1717Missing : Interacts normally with Rnp4, reconstitutes RNase P activity. 1 PublicationAdd
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 127127Ribonuclease P protein component 1PRO_0000128436Add
    BLAST

    Proteomic databases

    PRIDEiQ8U007.

    Interactioni

    Subunit structurei

    Consists of a catalytic RNA component and at least 4 protein subunits. Forms a subcomplex with Rnp4 which stimulates the catalytic RNA.5 Publications

    Protein-protein interaction databases

    STRINGi186497.PF1816.

    Structurei

    Secondary structure

    1
    127
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni20 – 223Combined sources
    Beta strandi23 – 253Combined sources
    Helixi27 – 315Combined sources
    Helixi40 – 434Combined sources
    Beta strandi52 – 598Combined sources
    Turni61 – 655Combined sources
    Beta strandi66 – 749Combined sources
    Beta strandi77 – 826Combined sources
    Beta strandi85 – 884Combined sources
    Beta strandi91 – 10010Combined sources
    Turni101 – 1033Combined sources
    Turni110 – 1134Combined sources
    Helixi117 – 1215Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KI7NMR-A1-127[»]
    ProteinModelPortaliQ8U007.
    SMRiQ8U007. Positions 36-127.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8U007.

    Family & Domainsi

    Domaini

    Interaction with Rnp4 is mediated by the N-terminus; short deletion mutants are still able to reconstitute RNase P activity.1 Publication

    Sequence similaritiesi

    Belongs to the eukaryotic/archaeal RNase P protein component 1 family.UniRule annotation

    Phylogenomic databases

    eggNOGiarCOG00784. Archaea.
    COG1588. LUCA.
    HOGENOMiHOG000231353.
    KOiK03538.
    OMAiWHELIGL.

    Family and domain databases

    Gene3Di2.30.30.210. 1 hit.
    HAMAPiMF_00754. RNase_P_1. 1 hit.
    InterProiIPR002730. RNase_P/MRP_p29.
    IPR023538. RNP1.
    IPR023534. Rof/RNase_P-like.
    [Graphical view]
    PfamiPF01868. UPF0086. 1 hit.
    [Graphical view]
    SMARTiSM00538. POP4. 1 hit.
    [Graphical view]
    SUPFAMiSSF101744. SSF101744. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8U007-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MWRNSEEREN RTSGRSQGSY QEIIGRTWIF RGAHRGRVNK KNIVWHELIG
    60 70 80 90 100
    LKVRVVNSTH PGYVGIEGYV IDETRNMLVI AGENKVWKVP KDVCIFEFET
    110 120
    WDGTKIKISG EKLVGRPEMR LKKRWRK
    Length:127
    Mass (Da):14,953
    Last modified:June 1, 2002 - v1
    Checksum:i3CBBA84DD7AE7E4C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81940.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81940; AAL81940; PF1816.
    KEGGipfu:PF1816.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81940.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KI7NMR-A1-127[»]
    ProteinModelPortaliQ8U007.
    SMRiQ8U007. Positions 36-127.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi186497.PF1816.

    Proteomic databases

    PRIDEiQ8U007.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL81940; AAL81940; PF1816.
    KEGGipfu:PF1816.

    Phylogenomic databases

    eggNOGiarCOG00784. Archaea.
    COG1588. LUCA.
    HOGENOMiHOG000231353.
    KOiK03538.
    OMAiWHELIGL.

    Miscellaneous databases

    EvolutionaryTraceiQ8U007.

    Family and domain databases

    Gene3Di2.30.30.210. 1 hit.
    HAMAPiMF_00754. RNase_P_1. 1 hit.
    InterProiIPR002730. RNase_P/MRP_p29.
    IPR023538. RNP1.
    IPR023534. Rof/RNase_P-like.
    [Graphical view]
    PfamiPF01868. UPF0086. 1 hit.
    [Graphical view]
    SMARTiSM00538. POP4. 1 hit.
    [Graphical view]
    SUPFAMiSSF101744. SSF101744. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRNP1_PYRFU
    AccessioniPrimary (citable) accession number: Q8U007
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2004
    Last sequence update: June 1, 2002
    Last modified: November 11, 2015
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.