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Protein

Ribonuclease P protein component 1

Gene

rnp1

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. The RNA is catalytic, but its KM for pre-tRNA is 170-fold decreased in the presence of the 4 known protein subunits (Rnp1-4). The protein subunits also decrease the amount of Mg2+ needed for activity.UniRule annotation3 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation1 Publication

Kineticsi

kcat is 9.5 min(-1). For enzyme reconstituted with RNA and 4 known subunits (Rnp1-4).
  1. KM=0.18 µM for E.coli pre-tRNA(Tyr)1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionEndonuclease, Hydrolase, Nuclease
    Biological processtRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein component 1UniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P component 1UniRule annotation
    Alternative name(s):
    Rpp29UniRule annotation
    Gene namesi
    Name:rnp1UniRule annotation
    Ordered Locus Names:PF1816
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    Proteomesi
    • UP000001013 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi1 – 31Missing : No interaction with Rnp4, does not reconstitute RNase P activity. 1 PublicationAdd BLAST31
    Mutagenesisi1 – 24Missing : No interaction with Rnp4, reconstitutes RNase P activity. 1 PublicationAdd BLAST24
    Mutagenesisi1 – 17Missing : Interacts normally with Rnp4, reconstitutes RNase P activity. 1 PublicationAdd BLAST17

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001284361 – 127Ribonuclease P protein component 1Add BLAST127

    Proteomic databases

    PRIDEiQ8U007

    Interactioni

    Subunit structurei

    Consists of a catalytic RNA component and at least 4 protein subunits. Forms a subcomplex with Rnp4 which stimulates the catalytic RNA.5 Publications

    Protein-protein interaction databases

    STRINGi186497.PF1816

    Structurei

    Secondary structure

    1127
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Turni20 – 22Combined sources3
    Beta strandi23 – 25Combined sources3
    Helixi27 – 31Combined sources5
    Helixi40 – 43Combined sources4
    Beta strandi52 – 59Combined sources8
    Turni61 – 65Combined sources5
    Beta strandi66 – 74Combined sources9
    Beta strandi77 – 82Combined sources6
    Beta strandi85 – 88Combined sources4
    Beta strandi91 – 100Combined sources10
    Turni101 – 103Combined sources3
    Turni110 – 113Combined sources4
    Helixi117 – 121Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2KI7NMR-A1-127[»]
    ProteinModelPortaliQ8U007
    SMRiQ8U007
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8U007

    Family & Domainsi

    Domaini

    Interaction with Rnp4 is mediated by the N-terminus; short deletion mutants are still able to reconstitute RNase P activity.1 Publication

    Sequence similaritiesi

    Belongs to the eukaryotic/archaeal RNase P protein component 1 family.UniRule annotation

    Phylogenomic databases

    eggNOGiarCOG00784 Archaea
    COG1588 LUCA
    HOGENOMiHOG000231353
    KOiK03538
    OMAiWHELIGL
    OrthoDBiPOG093Z0M9M

    Family and domain databases

    Gene3Di2.30.30.210, 1 hit
    HAMAPiMF_00754 RNase_P_1, 1 hit
    InterProiView protein in InterPro
    IPR002730 RNase_P/MRP_p29
    IPR036980 RNase_P/MRP_p29_sf
    IPR023538 RNP1
    IPR023534 Rof/RNase_P-like
    PfamiView protein in Pfam
    PF01868 UPF0086, 1 hit
    SMARTiView protein in SMART
    SM00538 POP4, 1 hit
    SUPFAMiSSF101744 SSF101744, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q8U007-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MWRNSEEREN RTSGRSQGSY QEIIGRTWIF RGAHRGRVNK KNIVWHELIG
    60 70 80 90 100
    LKVRVVNSTH PGYVGIEGYV IDETRNMLVI AGENKVWKVP KDVCIFEFET
    110 120
    WDGTKIKISG EKLVGRPEMR LKKRWRK
    Length:127
    Mass (Da):14,953
    Last modified:June 1, 2002 - v1
    Checksum:i3CBBA84DD7AE7E4C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE009950 Genomic DNA Translation: AAL81940.1

    Genome annotation databases

    EnsemblBacteriaiAAL81940; AAL81940; PF1816
    KEGGipfu:PF1816
    PATRICifig|186497.12.peg.1887

    Similar proteinsi

    Entry informationi

    Entry nameiRNP1_PYRFU
    AccessioniPrimary (citable) accession number: Q8U007
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2004
    Last sequence update: June 1, 2002
    Last modified: May 23, 2018
    This is version 83 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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