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Protein

tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS

Gene

tiaS

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.UniRule annotation

Catalytic activityi

ATP + agmatine + (tRNA(Ile)2)-cytidine(34) + H2O = (tRNA(Ile)2)-2-agmatinylcytidine(34) + AMP + 2 phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi268 – 34174OBUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

tRNA processingUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile2) 2-agmatinylcytidine synthetase TiaSUniRule annotation (EC:6.3.4.22UniRule annotation)
Short name:
tRNA(Ile2)-agm2C synthetaseUniRule annotation
Alternative name(s):
tRNA(Ile2) agmatidine synthetaseUniRule annotation
Gene namesi
Name:tiaSUniRule annotation
Ordered Locus Names:PF1855Imported
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)Imported
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiQ8TZX0.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF1855.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3U02X-ray2.40A/B/C/D1-252[»]
ProteinModelPortaliQ8TZX0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini140 – 253114DUF1743InterPro annotationAdd
BLAST
Domaini268 – 34174OB DNA-bindingInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TiaS family.UniRule annotation
Contains 1 OB DNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01115. Archaea.
COG1571. LUCA.
HOGENOMiHOG000225999.
KOiK06932.
OMAiGMCTTYL.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_01892. tRNA_Ile2_agm2C_synt. 1 hit.
InterProiIPR013696. DUF1743.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR024913. tRNA_Ile2__agm2C_synt.
[Graphical view]
PfamiPF08489. DUF1743. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TZX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIHIGIDDT DSPNGMCTTY IGAILYREIS KIAEPLDFPR LIRLNPNVPY
60 70 80 90 100
KTRGNGAVAM SFKIDEEKIK EVKTLVIRYV RELADIDHEN TNPGIVFLIG
110 120 130 140 150
EVPKELEEFS LRALREHVTI EEAEHVARKV NAEVYKFKLG RGIIGGLAAI
160 170 180 190 200
GYPLEKFTYE LLAYRKREYW GTPRRVIKES VFYADKWSYP FTYDNVDPYK
210 220 230 240 250
RTVLITPHGK DPVLVGIRGI DVGKILQVFE MIKIEEPIEF FQVYKTNQNT
260 270 280 290 300
DDHLVVKKIK DVKPYDNVVI RGKVAGKYWE KGRHVFFELE DETGKIRVAA
310 320 330 340 350
FEPTKKFRNY VRKLLPGDEV IIAGGVKEFE GVLTVNLEKF YPVNLVPKVE
360 370 380 390 400
YEKPRCPKCG GTMKSKGDYL KCKKCGYKME KILTPVKRPR DLKKKIYEVP
410 420
PDARKHISRP LVLPGGEDKI LEAL
Length:424
Mass (Da):48,751
Last modified:June 1, 2002 - v1
Checksum:iA73FBC2D4B6249B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81979.1.
RefSeqiWP_011012995.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81979; AAL81979; PF1855.
GeneIDi1469734.
KEGGipfu:PF1855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81979.1.
RefSeqiWP_011012995.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3U02X-ray2.40A/B/C/D1-252[»]
ProteinModelPortaliQ8TZX0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1855.

Proteomic databases

PRIDEiQ8TZX0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81979; AAL81979; PF1855.
GeneIDi1469734.
KEGGipfu:PF1855.

Phylogenomic databases

eggNOGiarCOG01115. Archaea.
COG1571. LUCA.
HOGENOMiHOG000225999.
KOiK06932.
OMAiGMCTTYL.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_01892. tRNA_Ile2_agm2C_synt. 1 hit.
InterProiIPR013696. DUF1743.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR024913. tRNA_Ile2__agm2C_synt.
[Graphical view]
PfamiPF08489. DUF1743. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ8TZX0_PYRFU
AccessioniPrimary (citable) accession number: Q8TZX0
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: December 9, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.