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Protein

tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS

Gene

tiaS

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.UniRule annotation

Catalytic activityi

ATP + agmatine + (tRNA(Ile)2)-cytidine(34) + H2O = (tRNA(Ile)2)-2-agmatinylcytidine(34) + AMP + 2 phosphate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processtRNA processingUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile2) 2-agmatinylcytidine synthetase TiaSUniRule annotation (EC:6.3.4.22UniRule annotation)
Short name:
tRNA(Ile2)-agm2C synthetaseUniRule annotation
Alternative name(s):
tRNA(Ile2) agmatidine synthetaseUniRule annotation
Gene namesi
Name:tiaSUniRule annotation
Ordered Locus Names:PF1855Imported
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)Imported
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiQ8TZX0.

Interactioni

Protein-protein interaction databases

STRINGi186497.PF1855.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3U02X-ray2.40A/B/C/D1-252[»]
ProteinModelPortaliQ8TZX0.
SMRiQ8TZX0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 253DUF1743InterPro annotationAdd BLAST114
Domaini268 – 341OBInterPro annotationAdd BLAST74

Sequence similaritiesi

Belongs to the TiaS family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01115. Archaea.
COG1571. LUCA.
HOGENOMiHOG000225999.
KOiK06932.
OMAiGMCTTYL.
OrthoDBiPOG093Z06XL.

Family and domain databases

HAMAPiMF_01892. tRNA_Ile2_agm2C_synt. 1 hit.
InterProiView protein in InterPro
IPR013696. DUF1743.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
IPR024913. tRNA_Ile2__agm2C_synt.
PfamiView protein in Pfam
PF08489. DUF1743. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TZX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIHIGIDDT DSPNGMCTTY IGAILYREIS KIAEPLDFPR LIRLNPNVPY
60 70 80 90 100
KTRGNGAVAM SFKIDEEKIK EVKTLVIRYV RELADIDHEN TNPGIVFLIG
110 120 130 140 150
EVPKELEEFS LRALREHVTI EEAEHVARKV NAEVYKFKLG RGIIGGLAAI
160 170 180 190 200
GYPLEKFTYE LLAYRKREYW GTPRRVIKES VFYADKWSYP FTYDNVDPYK
210 220 230 240 250
RTVLITPHGK DPVLVGIRGI DVGKILQVFE MIKIEEPIEF FQVYKTNQNT
260 270 280 290 300
DDHLVVKKIK DVKPYDNVVI RGKVAGKYWE KGRHVFFELE DETGKIRVAA
310 320 330 340 350
FEPTKKFRNY VRKLLPGDEV IIAGGVKEFE GVLTVNLEKF YPVNLVPKVE
360 370 380 390 400
YEKPRCPKCG GTMKSKGDYL KCKKCGYKME KILTPVKRPR DLKKKIYEVP
410 420
PDARKHISRP LVLPGGEDKI LEAL
Length:424
Mass (Da):48,751
Last modified:June 1, 2002 - v1
Checksum:iA73FBC2D4B6249B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81979.1.
RefSeqiWP_011012995.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81979; AAL81979; PF1855.
GeneIDi1469734.
KEGGipfu:PF1855.
PATRICifig|186497.12.peg.1926.

Similar proteinsi

Entry informationi

Entry nameiQ8TZX0_PYRFU
AccessioniPrimary (citable) accession number: Q8TZX0
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: September 27, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported