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Protein

Protein-L-isoaspartate O-methyltransferase

Gene

pcm

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.77. 5243.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate O-methyltransferase (EC:2.1.1.77)
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name:
PIMT
Gene namesi
Name:pcm
Ordered Locus Names:PF1922
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119231 – 219Protein-L-isoaspartate O-methyltransferaseAdd BLAST219

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi186497.PF1922.

Structurei

Secondary structure

1219
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 20Combined sources16
Helixi27 – 35Combined sources9
Helixi38 – 41Combined sources4
Helixi44 – 49Combined sources6
Beta strandi52 – 54Combined sources3
Beta strandi56 – 58Combined sources3
Helixi59 – 61Combined sources3
Beta strandi62 – 64Combined sources3
Helixi67 – 77Combined sources11
Beta strandi85 – 88Combined sources4
Helixi94 – 103Combined sources10
Beta strandi107 – 112Combined sources6
Helixi114 – 126Combined sources13
Beta strandi131 – 137Combined sources7
Helixi139 – 141Combined sources3
Helixi144 – 146Combined sources3
Beta strandi149 – 154Combined sources6
Beta strandi156 – 160Combined sources5
Helixi163 – 167Combined sources5
Beta strandi169 – 179Combined sources11
Beta strandi181 – 184Combined sources4
Beta strandi186 – 194Combined sources9
Beta strandi197 – 206Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JG1X-ray1.20A1-219[»]
1JG2X-ray1.50A1-219[»]
1JG3X-ray2.10A/B1-219[»]
1JG4X-ray1.50A1-219[»]
ProteinModelPortaliQ8TZR3.
SMRiQ8TZR3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TZR3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00976. Archaea.
COG2518. LUCA.
HOGENOMiHOG000257189.
KOiK00573.
OMAiPYTVAFQ.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TZR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDEKELYEK WMRTVEMLKA EGIIRSKEVE RAFLKYPRYL FVEDKYKKYA
60 70 80 90 100
HIDEPLPIPA GQTVSAPHMV AIMLEIANLK PGMNILEVGT GSGWNAALIS
110 120 130 140 150
EIVKTDVYTI ERIPELVEFA KRNLERAGVK NVHVILGDGS KGFPPKAPYD
160 170 180 190 200
VIIVTAGAPK IPEPLIEQLK IGGKLIIPVG SYHLWQELLE VRKTKDGIKI
210
KNHGGVAFVP LIGEYGWKE
Length:219
Mass (Da):24,617
Last modified:October 10, 2002 - v2
Checksum:iA239F32E24C3445D
GO

Sequence cautioni

The sequence AAL82046 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL82046.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiAAL82046; AAL82046; PF1922.
KEGGipfu:PF1922.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL82046.1. Different initiation.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JG1X-ray1.20A1-219[»]
1JG2X-ray1.50A1-219[»]
1JG3X-ray2.10A/B1-219[»]
1JG4X-ray1.50A1-219[»]
ProteinModelPortaliQ8TZR3.
SMRiQ8TZR3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1922.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL82046; AAL82046; PF1922.
KEGGipfu:PF1922.

Phylogenomic databases

eggNOGiarCOG00976. Archaea.
COG2518. LUCA.
HOGENOMiHOG000257189.
KOiK00573.
OMAiPYTVAFQ.

Enzyme and pathway databases

BRENDAi2.1.1.77. 5243.

Miscellaneous databases

EvolutionaryTraceiQ8TZR3.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00090. PIMT. 1 hit.
InterProiIPR000682. PCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11579. PTHR11579. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00080. pimt. 1 hit.
PROSITEiPS01279. PCMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIMT_PYRFU
AccessioniPrimary (citable) accession number: Q8TZR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: October 10, 2002
Last modified: November 2, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.