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Protein

Transcription elongation factor Spt5

Gene

spt5

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stimulates transcription elongation.UniRule annotation2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor Spt5UniRule annotation
Gene namesi
Name:spt5UniRule annotation
Ordered Locus Names:PF1990
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004221361 – 152Transcription elongation factor Spt5Add BLAST152

Proteomic databases

PRIDEiQ8TZK1

Interactioni

Subunit structurei

Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP) independently of nucleic acids. Forms a homodimer in solution.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
spt4Q8U4403EBI-8606402,EBI-8606370

Protein-protein interaction databases

DIPiDIP-59596N
IntActiQ8TZK1, 2 interactors
MINTiQ8TZK1
STRINGi186497.PF1990

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Helixi15 – 29Combined sources15
Beta strandi35 – 38Combined sources4
Beta strandi44 – 52Combined sources9
Helixi53 – 60Combined sources8
Beta strandi66 – 69Combined sources4
Helixi76 – 78Combined sources3
Helixi80 – 82Combined sources3
Turni88 – 91Combined sources4
Beta strandi97 – 100Combined sources4
Turni104 – 107Combined sources4
Beta strandi109 – 116Combined sources8
Turni117 – 120Combined sources4
Beta strandi121 – 128Combined sources8
Beta strandi134 – 138Combined sources5
Helixi139 – 141Combined sources3
Beta strandi142 – 146Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P8BX-ray1.80B/D1-152[»]
3QQCX-ray3.30D1-152[»]
ProteinModelPortaliQ8TZK1
SMRiQ8TZK1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TZK1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini94 – 124KOWUniRule annotationAdd BLAST31

Domaini

Composed of only a NusG N-terminal (NGN) domain and a KOW domain, similar to bacterial NusG. The NGN domain is the effector domain of the complex that mediates the interaction with RNAP. The NGN domain closes the RNAP active center cleft to lock nucleic acids and render the elongation complex stable and processive. The KOW domain may interact with RNA and/or other factors.2 Publications

Sequence similaritiesi

Belongs to the archaeal Spt5 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01920 Archaea
COG0250 LUCA
HOGENOMiHOG000229920
KOiK02601
OMAiFSEIEHF
OrthoDBiPOG093Z0DP6

Family and domain databases

Gene3Di2.30.30.30, 1 hit
3.30.70.940, 1 hit
HAMAPiMF_00950 Spt5_arch, 1 hit
InterProiView protein in InterPro
IPR005824 KOW
IPR006645 NGN_dom
IPR036735 NGN_dom_sf
IPR014722 Rib_L2_dom2
IPR005825 Ribosomal_L24/26_CS
IPR011590 Spt5_arc
IPR005100 TF_Spt5_NGN-domain
IPR008991 Translation_prot_SH3-like_sf
PfamiView protein in Pfam
PF00467 KOW, 1 hit
PF03439 Spt5-NGN, 1 hit
SMARTiView protein in SMART
SM00739 KOW, 1 hit
SM00738 NGN, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
TIGRFAMsiTIGR00405 KOW_elon_Spt5, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TZK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKIFAVRV THGQEETTAK LIYSKVRTYN LPIYAILAPS RVKGYIFVEA
60 70 80 90 100
PNKGVVDEAI RGIRHARGVL PGEVPFKEIE HFLEEKPAVS GLEPGDLVEV
110 120 130 140 150
IAGPFKGQKA KVVKIDESKD EVVVQFIDAI VPIPVTIKGD YVRLISKLQK

EE
Length:152
Mass (Da):16,792
Last modified:June 1, 2002 - v1
Checksum:iB9321A520404A450
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA Translation: AAL82114.1
RefSeqiWP_011013134.1, NC_003413.1

Genome annotation databases

EnsemblBacteriaiAAL82114; AAL82114; PF1990
GeneIDi1469874
KEGGipfu:PF1990
PATRICifig|186497.12.peg.2066

Similar proteinsi

Entry informationi

Entry nameiSPT5_PYRFU
AccessioniPrimary (citable) accession number: Q8TZK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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