Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription elongation factor Spt5

Gene

spt5

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates transcription elongation.UniRule annotation2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor Spt5UniRule annotation
Gene namesi
Name:spt5UniRule annotation
Ordered Locus Names:PF1990
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Transcription elongation factor Spt5PRO_0000422136Add
BLAST

Proteomic databases

PRIDEiQ8TZK1.

Interactioni

Subunit structurei

Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP) independently of nucleic acids. Forms a homodimer in solution.UniRule annotation2 Publications

Protein-protein interaction databases

DIPiDIP-59596N.
IntActiQ8TZK1. 1 interaction.
MINTiMINT-8173573.
STRINGi186497.PF1990.

Structurei

Secondary structure

1
152
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 107Combined sources
Helixi15 – 2915Combined sources
Beta strandi35 – 384Combined sources
Beta strandi44 – 529Combined sources
Helixi53 – 608Combined sources
Beta strandi66 – 694Combined sources
Helixi76 – 783Combined sources
Helixi80 – 823Combined sources
Turni88 – 914Combined sources
Beta strandi97 – 1004Combined sources
Turni104 – 1074Combined sources
Beta strandi109 – 1168Combined sources
Turni117 – 1204Combined sources
Beta strandi121 – 1288Combined sources
Beta strandi134 – 1385Combined sources
Helixi139 – 1413Combined sources
Beta strandi142 – 1465Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3P8BX-ray1.80B/D1-152[»]
3QQCX-ray3.30D1-152[»]
ProteinModelPortaliQ8TZK1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TZK1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 12431KOWUniRule annotationAdd
BLAST

Domaini

Composed of only a NusG N-terminal (NGN) domain and a KOW domain, similar to bacterial NusG. The NGN domain is the effector domain of the complex that mediates the interaction with RNAP. The NGN domain closes the RNAP active center cleft to lock nucleic acids and render the elongation complex stable and processive. The KOW domain may interact with RNA and/or other factors.2 Publications

Sequence similaritiesi

Belongs to the archaeal Spt5 family.UniRule annotation
Contains 1 KOW domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01920. Archaea.
COG0250. LUCA.
HOGENOMiHOG000229920.
KOiK02601.
OMAiPIPITIR.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
HAMAPiMF_00950. Spt5_arch.
InterProiIPR005824. KOW.
IPR006645. NGN_dom.
IPR014722. Rib_L2_dom2.
IPR005825. Ribosomal_L24/26_CS.
IPR011590. Spt5_arc.
IPR005100. TF_Spt5_NGN-domain.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PfamiPF00467. KOW. 1 hit.
PF03439. Spt5-NGN. 1 hit.
[Graphical view]
SMARTiSM00739. KOW. 1 hit.
SM00738. NGN. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
TIGRFAMsiTIGR00405. KOW_elon_Spt5. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TZK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKIFAVRV THGQEETTAK LIYSKVRTYN LPIYAILAPS RVKGYIFVEA
60 70 80 90 100
PNKGVVDEAI RGIRHARGVL PGEVPFKEIE HFLEEKPAVS GLEPGDLVEV
110 120 130 140 150
IAGPFKGQKA KVVKIDESKD EVVVQFIDAI VPIPVTIKGD YVRLISKLQK

EE
Length:152
Mass (Da):16,792
Last modified:June 1, 2002 - v1
Checksum:iB9321A520404A450
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL82114.1.
RefSeqiWP_011013134.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL82114; AAL82114; PF1990.
GeneIDi1469874.
KEGGipfu:PF1990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL82114.1.
RefSeqiWP_011013134.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3P8BX-ray1.80B/D1-152[»]
3QQCX-ray3.30D1-152[»]
ProteinModelPortaliQ8TZK1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59596N.
IntActiQ8TZK1. 1 interaction.
MINTiMINT-8173573.
STRINGi186497.PF1990.

Proteomic databases

PRIDEiQ8TZK1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL82114; AAL82114; PF1990.
GeneIDi1469874.
KEGGipfu:PF1990.

Phylogenomic databases

eggNOGiarCOG01920. Archaea.
COG0250. LUCA.
HOGENOMiHOG000229920.
KOiK02601.
OMAiPIPITIR.

Miscellaneous databases

EvolutionaryTraceiQ8TZK1.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
HAMAPiMF_00950. Spt5_arch.
InterProiIPR005824. KOW.
IPR006645. NGN_dom.
IPR014722. Rib_L2_dom2.
IPR005825. Ribosomal_L24/26_CS.
IPR011590. Spt5_arc.
IPR005100. TF_Spt5_NGN-domain.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PfamiPF00467. KOW. 1 hit.
PF03439. Spt5-NGN. 1 hit.
[Graphical view]
SMARTiSM00739. KOW. 1 hit.
SM00738. NGN. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
TIGRFAMsiTIGR00405. KOW_elon_Spt5. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity."
    Martinez-Rucobo F.W., Sainsbury S., Cheung A.C., Cramer P.
    EMBO J. 30:1302-1310(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.30 ANGSTROMS) IN COMPLEX WITH SPT4 AND RNAP, FUNCTION, SUBUNIT, DOMAIN.
  3. "RNA polymerase and transcription elongation factor Spt4/5 complex structure."
    Klein B.J., Bose D., Baker K.J., Yusoff Z.M., Zhang X., Murakami K.S.
    Proc. Natl. Acad. Sci. U.S.A. 108:546-550(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH SPT4, FUNCTION, SUBUNIT, DOMAIN.

Entry informationi

Entry nameiSPT5_PYRFU
AccessioniPrimary (citable) accession number: Q8TZK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.