ATP-dependent RNA helicase, putative - Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Preferred order of sections

Names and origin

Protein names

Submitted name:
ATP-dependent RNA helicase, putative EMBL AAL82139.1

Gene names

Ordered Locus Names:

PF2015

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	ATP-binding Calcium PDB 1J24 Metal-binding PDB 1J24 Nucleotide-binding
Molecular function	Helicase RuleBase RU000452 EMBL AAL82139.1 Hydrolase
Technical term	3D-structure PDB 1J24 PDB 1J25 PDB 1J22 PDB 1J23 PDB 1WP9 PDB 1X2I Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

584

1

Calcium PDB 1J24

Metal binding

594

1

Calcium PDB 1J24

Metal binding

595

1

Calcium; via carbonyl oxygen PDB 1J24

Sequences

Sequence

Length

Mass (Da)

Tools

Q8TZH8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 0561F58EB8E15679
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FASTA

764

86,939

        10         20         30         40         50         60 
MMLLRRDLIQ PRIYQEVIYA KCKETNCLIV LPTGLGKTLI AMMIAEYRLT KYGGKVLMLA 

        70         80         90        100        110        120 
PTKPLVLQHA ESFRRLFNLP PEKIVALTGE KSPEERSKAW ARAKVIVATP QTIENDLLAG 

       130        140        150        160        170        180 
RISLEDVSLI VFDEAHRAVG NYAYVFIARE YKRQAKNPLV IGLTASPGST PEKIMEVINN 

       190        200        210        220        230        240 
LGIEHIEYRS ENSPDVRPYV KGIRFEWVRV DLPEIYKEVR KLLREMLRDA LKPLAETGLL 

       250        260        270        280        290        300 
ESSSPDIPKK EVLRAGQIIN EEMAKGNHDL RGLLLYHAMA LKLHHAIELL ETQGLSALRA 

       310        320        330        340        350        360 
YIKKLYEEAK AGSTKASKEI FSDKRMKKAI SLLVQAKEIG LDHPKMDKLK EIIREQLQRK 

       370        380        390        400        410        420 
QNSKIIVFTN YRETAKKIVN ELVKDGIKAK RFVGQASKEN DRGLSQREQK LILDEFARGE 

       430        440        450        460        470        480 
FNVLVATSVG EEGLDVPEVD LVVFYEPVPS AIRSIQRRGR TGRHMPGRVI ILMAKGTRDE 

       490        500        510        520        530        540 
AYYWSSRQKE KIMQETIAKV SQAIKKQKQT SLVDFVREKE SEKTSLDKWL KKEKEEATEK 

       550        560        570        580        590        600 
EEKKVKAQEG VKVVVDSREL RSEVVKRLKL LGVKLEVKTL DVGDYIISED VAIERKSAND 

       610        620        630        640        650        660 
FIQSIIDGRL FDQVKRLKEA YSRPIMIVEG SLYGIRNVHP NAIRGAIAAV TVDFGVPIIF 

       670        680        690        700        710        720 
SSTPEETAQY IFLIAKREQE EREKPVRIRS EKKALTLAER QRLIVEGLPH VSATLARRLL 

       730        740        750        760 
KHFGSVERVF TASVAELMKV EGIGEKIAKE IRRVITAPYI EDEE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"X-ray and biochemical anatomy of an archaeal XPF/Rad1/Mus81 family nuclease: similarity between its endonuclease domain and restriction enzymes." Nishino T., Komori K., Ishino Y., Morikawa K. Structure 11:445-457(2003) [PubMed: 12679022] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 548-689 IN COMPLEX WITH CALCIUM.
[2]	"Crystal structure and functional implications of Pyrococcus furiosus hef helicase domain involved in branched DNA processing." Nishino T., Komori K., Tsuchiya D., Ishino Y., Morikawa K. Structure 13:143-153(2005) [PubMed: 15642269] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) OF 4-495.
[3]	"Structural and functional analyses of an archaeal XPF/Rad1/Mus81 nuclease: asymmetric DNA binding and cleavage mechanisms." Nishino T., Komori K., Ishino Y., Morikawa K. Structure 13:1183-1192(2005) [PubMed: 16084390] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 691-764.
[4]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE009950 Genomic DNA. Translation: AAL82139.1.

RefSeq

NP_579744.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J22	X-ray	1.80	A	548-689	[»]
1J23	X-ray	1.78	A	548-689	[»]
1J24	X-ray	1.78	A	548-689	[»]
1J25	X-ray	1.78	A	548-689	[»]
1WP9	X-ray	2.90	A/B/C/D/E/F	4-495	[»]
1X2I	X-ray	1.45	A/B	691-764	[»]

ProteinModelPortal

Q8TZH8.

SMR

Q8TZH8. Positions 2-487, 550-682, 694-761.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48431N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBPYRT00000005378; EBPYRP00000005239; EBPYRG00000005377.

GeneID

1469900.

GenomeReviews

Gene locus PF2015 in contig AE009950_GR.

KEGG

pfu:PF2015.

NMPDR

fig|186497.1.peg.2070.

Phylogenomic databases

GeneTree

EBGT00050000022150.

HOGENOM

HBG321504.

OMA

FYEPVPS.

PhylomeDB

Q8TZH8.

ProtClustDB

PRK13766.

Family and domain databases

InterPro

IPR014001. DEAD-like_helicase.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR001650. Helicase_C.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR011335. Restrct_endonuc_II-like.
IPR010994. RuvA_2-like.
[Graphical view]

Gene3D

G3DSA:3.40.50.10130. DNA_repair_nuc_XPF/helicase. 1 hit.

KO

K10896.

Pfam

PF00270. DEAD. 1 hit.
PF02732. ERCC4. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00891. ERCC4. 1 hit.
SM00490. HELICc. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]

SUPFAM

SSF52980. Restrict_endonuc_II-like_core. 1 hit.
SSF47781. RuvA_2_like. 1 hit.

PROSITE

PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q8TZH8_PYRFU

Accession

Primary (citable) accession number: Q8TZH8

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)