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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102ATPUniRule annotation1
Active sitei318Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi292 – 296ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:MK0001
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564271 – 352RNA 3'-terminal phosphate cyclaseAdd BLAST352

Interactioni

Protein-protein interaction databases

STRINGi190192.MK0001

Structurei

3D structure databases

ProteinModelPortaliQ8TZC9
SMRiQ8TZC9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04125 Archaea
COG0430 LUCA
HOGENOMiHOG000015264
KOiK18105
OMAiANRPKPG
OrthoDBiPOG093Z05RD

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 2 hits
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TZC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLIEIDGSY GEGGGQILRT AVGMSALTGE PVRIYNIRAN RPRPGLSHQH
60 70 80 90 100
LHAVKAVAEI CDAECEGLEI GSTEIVFEPG KVKGGEYEVD IGTAGSVTLL
110 120 130 140 150
LQAVKLAAIA ADGPVEMEVR GGTDVKWSPP VDYEINVNAH YLDRLGYRYE
160 170 180 190 200
LEVLRRGHYP RGGGIVRARM EPPKRLKPLE AVKFGELESV RGISHCVRLP
210 220 230 240 250
PHVAERQAKA ASEIIERELG IRPEIEIETY PKGRDPHLGP GSGIVLWAED
260 270 280 290 300
DQGNRIGADA LGEKGKPAEV VGREAAEQLV QRLRTGMALD EHMGDQILPF
310 320 330 340 350
LAIADGESVF GVTGVDPHLP TNAWVVEKFL PVSVEIRGKE GEPATVEVRP

EG
Length:352
Mass (Da):38,092
Last modified:June 1, 2002 - v1
Checksum:i9E0952828B031E5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA Translation: AAM01218.1

Genome annotation databases

EnsemblBacteriaiAAM01218; AAM01218; MK0001
KEGGimka:MK0001
PATRICifig|190192.8.peg.1

Similar proteinsi

Entry informationi

Entry nameiRTCA_METKA
AccessioniPrimary (citable) accession number: Q8TZC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health