Reviewed, UniProtKB/Swiss-Prot Q8TZC9 (RTCA_METKA)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	MK0001

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156427
Sites
	Active site	318	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TZC9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 9E0952828B031E5A	FASTA	352	38,092
	10 20 30 40 50 60 MSLIEIDGSY GEGGGQILRT AVGMSALTGE PVRIYNIRAN RPRPGLSHQH LHAVKAVAEI 70 80 90 100 110 120 CDAECEGLEI GSTEIVFEPG KVKGGEYEVD IGTAGSVTLL LQAVKLAAIA ADGPVEMEVR 130 140 150 160 170 180 GGTDVKWSPP VDYEINVNAH YLDRLGYRYE LEVLRRGHYP RGGGIVRARM EPPKRLKPLE 190 200 210 220 230 240 AVKFGELESV RGISHCVRLP PHVAERQAKA ASEIIERELG IRPEIEIETY PKGRDPHLGP 250 260 270 280 290 300 GSGIVLWAED DQGNRIGADA LGEKGKPAEV VGREAAEQLV QRLRTGMALD EHMGDQILPF 310 320 330 340 350 LAIADGESVF GVTGVDPHLP TNAWVVEKFL PVSVEIRGKE GEPATVEVRP EG

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009439 Genomic DNA. Translation: AAM01218.1.
RefSeq	NP_613288.1.
3D structure databases
HSSP	HSSP built from PDB template 1QMH based on UniProtKB P46849.
ModBase	Search...
Genome annotation databases
GeneID	1477327.
GenomeReviews	Gene locus MK0001 in contig AE009439_GR.
KEGG	mka:MK0001.
NMPDR	fig|190192.1.peg.1.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TZC9.
OMA	EIDGSYG.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0001-MON.
BRENDA	6.5.1.4. 7577.
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_METKA

Accession

Primary (citable) accession number: Q8TZC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)