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Protein

Replication factor C small subunit

Gene

rfcS

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 588ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: InterPro
  3. four-way junction helicase activity Source: InterPro

GO - Biological processi

  1. DNA recombination Source: InterPro
  2. DNA repair Source: InterPro
  3. DNA replication Source: UniProtKB-KW
  4. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C small subunit
Short name:
RFC small subunit
Alternative name(s):
Clamp loader small subunit
Cleaved into the following chain:
Gene namesi
Name:rfcS
Ordered Locus Names:MK0006
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8282Replication factor C small subunit, 1st partSequence AnalysisPRO_0000030368Add
BLAST
Chaini83 – 387305Mkn RFC inteinSequence AnalysisPRO_0000030369Add
BLAST
Chaini388 – 635248Replication factor C small subunit, 2nd partSequence AnalysisPRO_0000030370Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).By similarity

Protein-protein interaction databases

STRINGi190192.MK0006.

Structurei

3D structure databases

ProteinModelPortaliQ8TZC4.
SMRiQ8TZC4. Positions 5-83, 385-629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000154101.
KOiK04801.
OMAiNWRYEFR.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006142. INTEIN.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
[Graphical view]
PfamiPF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TZC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEHELRVLE IPWVEKYRPK RLDDIVDQEH VVERLKAYVN RGDMPNLLFA
60 70 80 90 100
GPPGTGKTTA ALCLARELFG EHWRDNFLEL NASVSADTPI LVRRGGEVLR
110 120 130 140 150
VTFEDLDSWY FGDRGGEYVD VSDLEVLTVD RNFRVTWARV SKLIRHRARK
160 170 180 190 200
ILRVHLEDGT IELTGNHAVM VLDEGGLRAV KASEIEEGSF LLSFVAELDE
210 220 230 240 250
QPTDGGTVVT SVGSGSRVSD TTYELPVEVR VELLRELADD GVIEASEDVS
260 270 280 290 300
VDLAWLARIS GVESRVTDDG VELVWETRTG DLLPADPVLK LVERLESDLV
310 320 330 340 350
DDLESWVFDG RVSKEAVRKV LSSVDAKNLR GDARRAYRML RTLVRSDVHA
360 370 380 390 400
VKVEDLDVMD YDGYVYDVSV PGNEMFFAGE VPVLLHNSDE RGIDVIRTKV
410 420 430 440 450
KNFARTRPMG GARFKIIFLD EADNLTRDSQ QALRRIMEMY SDACRFILAA
460 470 480 490 500
NYSSAIIDPI QSRCVVFKFT KLPESAIKER LRKIAESEGV EITEDALDAI
510 520 530 540 550
VYVSEGDMRR AINVLQAAAA LGREIDEDTV FQIAATARPE EVREMIHHAW
560 570 580 590 600
NGDFERAREL LHELLTRYGM SGEDVVRQVH REIFDMDEIP EEAIPELVNA
610 620 630
VGDFEYRLIR GSDERIQLEA LLARIHALGN EYSGG
Length:635
Mass (Da):71,586
Last modified:May 31, 2002 - v1
Checksum:i9AC69359719E08C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM01223.1.
RefSeqiNP_613293.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM01223; AAM01223; MK0006.
GeneIDi1477308.
KEGGimka:MK0006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM01223.1.
RefSeqiNP_613293.1. NC_003551.1.

3D structure databases

ProteinModelPortaliQ8TZC4.
SMRiQ8TZC4. Positions 5-83, 385-629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190192.MK0006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM01223; AAM01223; MK0006.
GeneIDi1477308.
KEGGimka:MK0006.

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000154101.
KOiK04801.
OMAiNWRYEFR.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006142. INTEIN.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
[Graphical view]
PfamiPF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiRFCS_METKA
AccessioniPrimary (citable) accession number: Q8TZC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2004
Last sequence update: May 31, 2002
Last modified: March 31, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.