Replication factor C small subunit - Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

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Q8TZC4 (RFCS_METKA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 60. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Replication factor C small subunit
Short name=RFC small subunit

Alternative name(s):
Clamp loader small subunit

Cleaved into the following chain:

Mkn RFC intein

Gene names

Name:	rfcS
Ordered Locus Names:	MK0006

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA By similarity. HAMAP MF_01509
Subunit structure	Heteromultimer composed of small subunits (rfcS) and large subunits (rfcL) By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP MF_01509
Sequence similarities	Belongs to the activator 1 small subunits family. RfcS subfamily.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro DNA replication factor C complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA clamp loader activity Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 82

Replication factor C small subunit, 1st part Potential

PRO_0000030368

Chain

83 – 387

305

Mkn RFC intein Potential

PRO_0000030369

Chain

388 – 635

248

Replication factor C small subunit, 2nd part Potential

PRO_0000030370

Regions

Nucleotide binding

51 – 58

ATP Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q8TZC4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 9AC69359719E08C5
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FASTA

635

71,586

        10         20         30         40         50         60 
MAEHELRVLE IPWVEKYRPK RLDDIVDQEH VVERLKAYVN RGDMPNLLFA GPPGTGKTTA 

        70         80         90        100        110        120 
ALCLARELFG EHWRDNFLEL NASVSADTPI LVRRGGEVLR VTFEDLDSWY FGDRGGEYVD 

       130        140        150        160        170        180 
VSDLEVLTVD RNFRVTWARV SKLIRHRARK ILRVHLEDGT IELTGNHAVM VLDEGGLRAV 

       190        200        210        220        230        240 
KASEIEEGSF LLSFVAELDE QPTDGGTVVT SVGSGSRVSD TTYELPVEVR VELLRELADD 

       250        260        270        280        290        300 
GVIEASEDVS VDLAWLARIS GVESRVTDDG VELVWETRTG DLLPADPVLK LVERLESDLV 

       310        320        330        340        350        360 
DDLESWVFDG RVSKEAVRKV LSSVDAKNLR GDARRAYRML RTLVRSDVHA VKVEDLDVMD 

       370        380        390        400        410        420 
YDGYVYDVSV PGNEMFFAGE VPVLLHNSDE RGIDVIRTKV KNFARTRPMG GARFKIIFLD 

       430        440        450        460        470        480 
EADNLTRDSQ QALRRIMEMY SDACRFILAA NYSSAIIDPI QSRCVVFKFT KLPESAIKER 

       490        500        510        520        530        540 
LRKIAESEGV EITEDALDAI VYVSEGDMRR AINVLQAAAA LGREIDEDTV FQIAATARPE 

       550        560        570        580        590        600 
EVREMIHHAW NGDFERAREL LHELLTRYGM SGEDVVRQVH REIFDMDEIP EEAIPELVNA 

       610        620        630 
VGDFEYRLIR GSDERIQLEA LLARIHALGN EYSGG

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References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01223.1.
RefSeq	NP_613293.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TZC4.
SMR	Q8TZC4. Positions 5-83, 385-629.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1477308.
GenomeReviews	Gene locus MK0006 in contig AE009439_GR.
KEGG	mka:MK0006.
NMPDR	fig\|190192.1.peg.6.
Phylogenomic databases
HOGENOM	HBG120083.
OMA	EMFFAGE.
PhylomeDB	Q8TZC4.
ProtClustDB	CLSK519869.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0006-MONOMER.
Family and domain databases
HAMAP	MF_01509. RfcS. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR008921. DNA_pol3_clamp-load_cplx_C. IPR010372. DNA_pol3_delta. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR006141. Intein_splice_site. IPR013748. Rep_factor-C. [Graphical view]
KO	K04801.
Pfam	PF06144. DNA_pol3_delta. 1 hit. PF08542. Rep_fac_C. 1 hit. PF05496. RuvB_N. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00382. AAA. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF48019. Pol_clamp_load_C. 1 hit.
TIGRFAMs	TIGR01443. Intein_Cterm. 1 hit. TIGR01445. Intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFCS_METKA

Accession

Primary (citable) accession number: Q8TZC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents