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Q8TZC4 (RFCS_METKA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replication factor C small subunit

Short name=RFC small subunit
Alternative name(s):
Clamp loader small subunit

Cleaved into the following chain:

  1. Mkn RFC intein
Gene names
Name:rfcS
Ordered Locus Names:MK0006
OrganismMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]
Taxonomic identifier190192 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus

Protein attributes

Sequence length635 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA By similarity.

Subunit structure

Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL) By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the activator 1 small subunits family. RfcS subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8282Replication factor C small subunit, 1st part Potential
PRO_0000030368
Chain83 – 387305Mkn RFC intein Potential
PRO_0000030369
Chain388 – 635248Replication factor C small subunit, 2nd part Potential
PRO_0000030370

Regions

Nucleotide binding51 – 588ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q8TZC4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 9AC69359719E08C5

FASTA63571,586
        10         20         30         40         50         60 
MAEHELRVLE IPWVEKYRPK RLDDIVDQEH VVERLKAYVN RGDMPNLLFA GPPGTGKTTA 

        70         80         90        100        110        120 
ALCLARELFG EHWRDNFLEL NASVSADTPI LVRRGGEVLR VTFEDLDSWY FGDRGGEYVD 

       130        140        150        160        170        180 
VSDLEVLTVD RNFRVTWARV SKLIRHRARK ILRVHLEDGT IELTGNHAVM VLDEGGLRAV 

       190        200        210        220        230        240 
KASEIEEGSF LLSFVAELDE QPTDGGTVVT SVGSGSRVSD TTYELPVEVR VELLRELADD 

       250        260        270        280        290        300 
GVIEASEDVS VDLAWLARIS GVESRVTDDG VELVWETRTG DLLPADPVLK LVERLESDLV 

       310        320        330        340        350        360 
DDLESWVFDG RVSKEAVRKV LSSVDAKNLR GDARRAYRML RTLVRSDVHA VKVEDLDVMD 

       370        380        390        400        410        420 
YDGYVYDVSV PGNEMFFAGE VPVLLHNSDE RGIDVIRTKV KNFARTRPMG GARFKIIFLD 

       430        440        450        460        470        480 
EADNLTRDSQ QALRRIMEMY SDACRFILAA NYSSAIIDPI QSRCVVFKFT KLPESAIKER 

       490        500        510        520        530        540 
LRKIAESEGV EITEDALDAI VYVSEGDMRR AINVLQAAAA LGREIDEDTV FQIAATARPE 

       550        560        570        580        590        600 
EVREMIHHAW NGDFERAREL LHELLTRYGM SGEDVVRQVH REIFDMDEIP EEAIPELVNA 

       610        620        630 
VGDFEYRLIR GSDERIQLEA LLARIHALGN EYSGG 

« Hide

References

[1]"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009439 Genomic DNA. Translation: AAM01223.1.
RefSeqNP_613293.1. NC_003551.1.

3D structure databases

ProteinModelPortalQ8TZC4.
SMRQ8TZC4. Positions 5-83, 385-629.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190192.MK0006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM01223; AAM01223; MK0006.
GeneID1477308.
KEGGmka:MK0006.

Phylogenomic databases

eggNOGCOG1372.
HOGENOMHOG000154101.
KOK04801.
OMANWRYEFR.

Family and domain databases

Gene3D2.170.16.10. 2 hits.
3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006142. INTEIN.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR013748. Rep_factorC_C_dom.
[Graphical view]
PfamPF08542. Rep_fac_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF48019. SSF48019. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFCS_METKA
AccessionPrimary (citable) accession number: Q8TZC4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries