Q8TZC4 (RFCS_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replication factor C small subunit
Short name=RFC small subunit

Alternative name(s):
Clamp loader small subunit

Cleaved into the following chain:

1. Mkn RFC intein

Gene names

Name:	rfcS
Ordered Locus Names:	MK0006

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus › Methanopyrus kandleri

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA By similarity. HAMAP-Rule MF_01509
Subunit structure	Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL) By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_01509
Sequence similarities	Belongs to the activator 1 small subunits family. RfcS subfamily.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 82	82	Replication factor C small subunit, 1st part Potential		PRO_0000030368
	Chain	83 – 387	305	Mkn RFC intein Potential		PRO_0000030369
	Chain	388 – 635	248	Replication factor C small subunit, 2nd part Potential		PRO_0000030370
Regions
	Nucleotide binding	51 – 58	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TZC4 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 9AC69359719E08C5	FASTA	635	71,586
	10 20 30 40 50 60 MAEHELRVLE IPWVEKYRPK RLDDIVDQEH VVERLKAYVN RGDMPNLLFA GPPGTGKTTA 70 80 90 100 110 120 ALCLARELFG EHWRDNFLEL NASVSADTPI LVRRGGEVLR VTFEDLDSWY FGDRGGEYVD 130 140 150 160 170 180 VSDLEVLTVD RNFRVTWARV SKLIRHRARK ILRVHLEDGT IELTGNHAVM VLDEGGLRAV 190 200 210 220 230 240 KASEIEEGSF LLSFVAELDE QPTDGGTVVT SVGSGSRVSD TTYELPVEVR VELLRELADD 250 260 270 280 290 300 GVIEASEDVS VDLAWLARIS GVESRVTDDG VELVWETRTG DLLPADPVLK LVERLESDLV 310 320 330 340 350 360 DDLESWVFDG RVSKEAVRKV LSSVDAKNLR GDARRAYRML RTLVRSDVHA VKVEDLDVMD 370 380 390 400 410 420 YDGYVYDVSV PGNEMFFAGE VPVLLHNSDE RGIDVIRTKV KNFARTRPMG GARFKIIFLD 430 440 450 460 470 480 EADNLTRDSQ QALRRIMEMY SDACRFILAA NYSSAIIDPI QSRCVVFKFT KLPESAIKER 490 500 510 520 530 540 LRKIAESEGV EITEDALDAI VYVSEGDMRR AINVLQAAAA LGREIDEDTV FQIAATARPE 550 560 570 580 590 600 EVREMIHHAW NGDFERAREL LHELLTRYGM SGEDVVRQVH REIFDMDEIP EEAIPELVNA 610 620 630 VGDFEYRLIR GSDERIQLEA LLARIHALGN EYSGG

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01223.1.
RefSeq	NP_613293.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TZC4.
SMR	Q8TZC4. Positions 5-83, 385-629.
ModBase	Search...
Protein-protein interaction databases
STRING	190192.MK0006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM01223; AAM01223; MK0006.
GeneID	1477308.
KEGG	mka:MK0006.
Phylogenomic databases
eggNOG	COG1372.
HOGENOM	HOG000154101.
KO	K04801.
OMA	EMFFAGE.
ProtClustDB	CLSK519869.
Family and domain databases
HAMAP	MF_01509. RfcS.
InterPro	IPR003593. AAA+_ATPase. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR008921. DNA_pol3_clamp-load_cplx_C. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006142. INTEIN. IPR006141. Intein_splice_site. IPR013748. Rep_factorC_C_dom. [Graphical view]
Pfam	PF08542. Rep_fac_C. 1 hit. PF05496. RuvB_N. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00382. AAA. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF48019. Pol_clamp_load_C. 1 hit.
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFCS_METKA

Accession

Primary (citable) accession number: Q8TZC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program