Q8TZ45 (ASPD_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase
EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	MK0094

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus › Methanopyrus kandleri

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP-Rule MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP-Rule MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP-Rule MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP-Rule MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	NAD binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Probable L-aspartate dehydrogenase HAMAP-Rule MF_01265		PRO_0000144897
Sites
	Active site	213	1	By similarity
	Binding site	128	1	NAD; via amide nitrogen By similarity
	Binding site	183	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TZ45 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 298438D77FC7BD75	FASTA	262	28,185
	10 20 30 40 50 60 MKKLSLALVG AGGIGTTVLR EIREGRLEGK VEPVLVCDRH PEKLKRIERW FPDCDTSTDL 70 80 90 100 110 120 DDAMSAEADV LLEAASVEAA ASLLPDALKR FDVIVMSVGA LVLEEGLLSR CREVAEVTGH 130 140 150 160 170 180 RLHVPSGAVG GLDVLRALRG RVREVTLTTI KPPKALNKDV SERTVLYEGS VRDAVRKFPK 190 200 210 220 230 240 NINVAAAVSL AVGDPSLVTV RIVCDPEVSV NTHVIEVESS AGTYRFELRN EALPDNPKTS 250 260 AVAAYSAVAL IERMTEGIRV GT

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01311.1.
RefSeq	NP_613381.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TZ45.
ModBase	Search...
Protein-protein interaction databases
STRING	190192.MK0094.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM01311; AAM01311; MK0094.
GeneID	1477397.
KEGG	mka:MK0094.
Phylogenomic databases
eggNOG	COG1712.
HOGENOM	HOG000206326.
KO	K06989.
OMA	VVECAPA.
Enzyme and pathway databases
UniPathway	UPA00253; UER00456.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_01265. NadX.
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR022487. Asp_DH_NAD_synth_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMs	TIGR03855. NAD_NadX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_METKA

Accession

Primary (citable) accession number: Q8TZ45

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program