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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityUniRule annotation
Active sitei609 – 6091For Fru-6P isomerization activityUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation (EC:2.6.1.16UniRule annotation)
Alternative name(s):
D-fructose-6-phosphate amidotransferaseUniRule annotation
GFATUniRule annotation
Glucosamine-6-phosphate synthaseUniRule annotation
Hexosephosphate aminotransferaseUniRule annotation
L-glutamine--D-fructose-6-phosphate amidotransferaseUniRule annotation
Gene namesi
Name:glmSUniRule annotation
Ordered Locus Names:MK0127
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 614613Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135425Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi190192.MK0127.

Structurei

3D structure databases

ProteinModelPortaliQ8TZ14.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 223222Glutamine amidotransferase type-2UniRule annotationAdd
BLAST
Domaini286 – 428143SIS 1UniRule annotationAdd
BLAST
Domaini463 – 604142SIS 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
Contains 2 SIS domains.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
KOiK00820.
OMAiGEFFCAS.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TZ14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGYTGE RDAAPIIVDS LVRLEYRGYD SAGVATIHEG RLYLEKDAGK
60 70 80 90 100
LTEGGEPTKL QRSLRKLPGK IGIGHTRWAT HGDPNRRNAH PHTDCRDEIA
110 120 130 140 150
VVHNGIIENF MQLREELEDK GHRFDSETDT EVVPHLIEQG MKEGKSFFEA
160 170 180 190 200
FVEAVRRLEG SYAIAAICTR EPDVILAARK ESPLVVGLGD DGNFLASDIP
210 220 230 240 250
AILPETNRVI PIDDGEIVVV KRDEVRILDA ETLEDVTEEK EVQIVEEDPH
260 270 280 290 300
TLERRGYPHF MLKEIHEQPE AVRNTLRIER ENLMEMAEEL VGGDYTKLYI
310 320 330 340 350
VACGTSYHAG LGAKYATELL AKFPVDVVIA SEFRYVTKEL VDENTLVLAI
360 370 380 390 400
SQSGETADTL AAVREANARG ATTIALTNVV GSTITREVDH VMYTHAGFEK
410 420 430 440 450
AVAATKTYTA QLAAMYTLAV ELARHFGEIT NKEAEEYHAE LNKVPEMLEE
460 470 480 490 500
VLSWEREREI AVMGGRYKER PNWFFIGRGP GYPTAMEGAL KLKEITYQHA
510 520 530 540 550
EAYPAGELKH GPLALIEEGV PVVAVAQPGG VYEKMLANIE EVKARGATVI
560 570 580 590 600
TVADEKDEAV EEHSDHVIRV PSISEVFSPI VYTVPLQLLA YYMSVARGID
610
PDYPRNLAKS VTVE
Length:614
Mass (Da):68,112
Last modified:January 23, 2007 - v3
Checksum:i46E24A0CC30CE1FA
GO

Sequence cautioni

The sequence AAM01344.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM01344.1. Different initiation.
RefSeqiNP_613414.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM01344; AAM01344; MK0127.
GeneIDi1477430.
KEGGimka:MK0127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM01344.1. Different initiation.
RefSeqiNP_613414.1. NC_003551.1.

3D structure databases

ProteinModelPortaliQ8TZ14.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190192.MK0127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM01344; AAM01344; MK0127.
GeneIDi1477430.
KEGGimka:MK0127.

Phylogenomic databases

eggNOGiCOG0449.
HOGENOMiHOG000258896.
KOiK00820.
OMAiGEFFCAS.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiGLMS_METKA
AccessioniPrimary (citable) accession number: Q8TZ14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.