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Protein

O-phospho-L-seryl-tRNA:Cys-tRNA synthase

Gene

MK0229

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).UniRule annotation

Catalytic activityi

O-phospho-L-seryl-tRNA(Cys) + sulfide = L-cysteinyl-tRNA(Cys) + phosphate.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851Sulfur donor or Sep-tRNA(Cys)UniRule annotation
Binding sitei109 – 1091Sulfur donor or Sep-tRNA(Cys)UniRule annotation
Binding sitei110 – 1101Sulfur donor or Sep-tRNA(Cys)UniRule annotation
Binding sitei191 – 1911Pyridoxal phosphateUniRule annotation
Binding sitei283 – 2831Sulfur donor or Sep-tRNA(Cys)UniRule annotation
Binding sitei292 – 2921Sulfur donor or Sep-tRNA(Cys)UniRule annotation

GO - Molecular functioni

  1. pyridoxal phosphate binding Source: InterPro
  2. Sep-tRNA:Cys-tRNA synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
O-phospho-L-seryl-tRNA:Cys-tRNA synthaseUniRule annotation (EC:2.5.1.73UniRule annotation)
Alternative name(s):
Sep-tRNA:Cys-tRNA synthaseUniRule annotation
Short name:
SepCysSUniRule annotation
Gene namesi
Ordered Locus Names:MK0229
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392O-phospho-L-seryl-tRNA:Cys-tRNA synthasePRO_0000359456Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei217 – 2171N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer. Interacts with SepRS.UniRule annotation

Protein-protein interaction databases

STRINGi190192.MK0229.

Structurei

3D structure databases

ProteinModelPortaliQ8TYR3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni84 – 852Pyridoxal phosphate bindingUniRule annotation
Regioni214 – 2163Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the SepCysS family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1103.
HOGENOMiHOG000015511.
KOiK06868.
OMAiLILNTAY.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
HAMAPiMF_01675. Sep_Cys_tRNA_synth.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR013375. Sep_Cys-tRNA_synth_arc.
IPR008829. SLA/LP_auto_ag.
[Graphical view]
PfamiPF05889. SLA_LP_auto_ag. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02539. SepCysS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TYR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLDRYRNIV RETERKYINV NPIQRGGVLT PEARKALLEF GDGYSVCDFC
60 70 80 90 100
EGLLHEIEKP PIRQFHEDLA EFLGMDVVRI TAGARYAKEA VMSALCEEGD
110 120 130 140 150
VVVADSLAHY TTFVAAEKAG ATVREVPNTG HPEYKVKVDE YARVIDEVED
160 170 180 190 200
ERGDPPALAL LTHVDSEYGN LADAEKFVKI CRKKGVPALL NCAYTMGRMD
210 220 230 240 250
LSNLSPKPDF MVGSGHKGMA ACAPCGVLAM REEWEEEVLR GSSLRGDVSG
260 270 280 290 300
REWPHKEVEM LGCTVMGAPI VTMMASFPHV VERVKRWKEE VRKTRWFVKE
310 320 330 340 350
MERIEGVRQL GERPKRHDLV KFETPGFHEV AEDHPRRGYF LYEELKKRGV
360 370 380 390
IGIQPGQTET IKASVYGLTD EQVEHVVRAF HEIAEEYGLE VS
Length:392
Mass (Da):44,252
Last modified:June 1, 2002 - v1
Checksum:i28CFB3206D0621BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM01446.1.
RefSeqiNP_613516.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM01446; AAM01446; MK0229.
GeneIDi1477532.
KEGGimka:MK0229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM01446.1.
RefSeqiNP_613516.1. NC_003551.1.

3D structure databases

ProteinModelPortaliQ8TYR3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190192.MK0229.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM01446; AAM01446; MK0229.
GeneIDi1477532.
KEGGimka:MK0229.

Phylogenomic databases

eggNOGiCOG1103.
HOGENOMiHOG000015511.
KOiK06868.
OMAiLILNTAY.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
HAMAPiMF_01675. Sep_Cys_tRNA_synth.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR013375. Sep_Cys-tRNA_synth_arc.
IPR008829. SLA/LP_auto_ag.
[Graphical view]
PfamiPF05889. SLA_LP_auto_ag. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02539. SepCysS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiSPSS_METKA
AccessioniPrimary (citable) accession number: Q8TYR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 1, 2002
Last modified: February 4, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.