Q8TYL5 (RISB_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	MK0281

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 141	141	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_0000134844

Sequences

	Sequence		Length	Mass (Da)
	Q8TYL5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: AB6E47CB7DA0C2CB	FASTA	141	15,697
	10 20 30 40 50 60 MSEIRLGLVV TEFNREITYA MEELAVQHAK DLGAKVVERV LVPGSFEVPL AVRKLLERDD 70 80 90 100 110 120 IDAVVTLGAI IKGDTDHDQA IAQQAFRKIQ DLMVEYGKPV ALGISGPGMT RMEALERVHY 130 140 AKRAVEAAVK MVRRLRELEG E

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01498.1.
RefSeq	NP_613568.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TYL5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1477584.
GenomeReviews	Gene locus MK0281 in contig AE009439_GR.
KEGG	mka:MK0281.
NMPDR	fig|190192.1.peg.281.
Phylogenomic databases
HOGENOM	HBG311126.
OMA	IQGATKH.
PhylomeDB	Q8TYL5.
ProtClustDB	PRK00061.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0281-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_METKA

Accession

Primary (citable) accession number: Q8TYL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program