Reviewed, UniProtKB/Swiss-Prot Q8TYL4 (PIMT_METKA)

Names and origin

Protein names

Recommended name:
    Protein-L-isoaspartate O-methyltransferase
    EC=2.1.1.77
Alternative name(s):
    Protein-beta-aspartate methyltransferase
      Short name=PIMT
    Protein L-isoaspartyl methyltransferase
    L-isoaspartyl protein carboxyl methyltransferase

Gene names

Name:	pcm
Ordered Locus Names:	MK0282

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: HAMAP protein repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Protein-L-isoaspartate O-methyltransferase HAMAP MF_00090		PRO_0000111917
Sites
	Active site	66	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TYL4-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 1BCD87E3265E61B4	FASTA	226	25,207
	10 20 30 40 50 60 MAEDDKVFRR ARERLVERLK SLGYIRSNRV AEAMLKVPRH EFVPEDLRDR AYVDSPLPIG 70 80 90 100 110 120 RGQTISAPHM VAIMTELLDP RPGHKVLEVG AGSGYHAAVV AELVKPDGRV ITVERIPELA 130 140 150 160 170 180 DFARNNLKKT GYDRFVKVLV GDGTKGYPPE APYDRILVTA GAPDVPESLL EQLKPGGKMV 190 200 210 220 IPVGDRHMQE LWLVEKTEDG EIRRRRHGGC AFVPLIGEEG FQEPES

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010326 Genomic DNA. Translation: AAM01499.1.
RefSeq	NP_613569.1.
3D structure databases
HSSP	HSSP built from PDB template 1JG1 based on UniProtKB Q8TZR3.
ModBase	Search...
Genome annotation databases
GeneID	1477585.
GenomeReviews	Gene locus MK0282 in contig AE009439_GR.
KEGG	mka:MK0282.
NMPDR	fig|190192.1.peg.282.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TYL4.
OMA	Q8TYL4. APYDRIY.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0282-MON.
BRENDA	2.1.1.77. 7577.
Family and domain databases
HAMAP	MF_00090. [Tree]
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PCMT. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_METKA

Accession

Primary (citable) accession number: Q8TYL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)