Q8TYD7 (GUAAB_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Synonyms:	guaA_1
Ordered Locus Names:	MK0363

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thiamine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140242
Regions
	Domain	2 – 186	185	GMPS ATP-PPase
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TYD7 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: B37326D571706311	FASTA	314	35,327
	10 20 30 40 50 60 MFDPKKFVEE AIEELRREIG DRKAIIAVSG GVDSTTAAVL THRAIGSHLV CVFVDHGFMR 70 80 90 100 110 120 KGEPERIREL LEEELGLNLR FVEAAEEFFE ALRGVTDPEE KRKIIGEKFI EVFERIAEEE 130 140 150 160 170 180 EAEVLVQGTI APDIIESERG IKSHHNVGGL PEKLNLDVVE PLRDLYKDEV REVARYLGIP 190 200 210 220 230 240 DEIVERMPFP GPGLAVRVLG EVTPEKVEIV REANAIVEEE VEKAVEEGKM SKPWQAFAAL 250 260 270 280 290 300 LDCKATGVKG DERDYGWVIA VRIVESIDAM IADVPEVPWE VLRNIQDRIT SEVPEVTRVL 310 FDITPKPPAT IEFE

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01578.1.
RefSeq	NP_613648.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TYD7.
SMR	Q8TYD7. Positions 3-314.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1477666.
GenomeReviews	Gene locus MK0363 in contig AE009439_GR.
KEGG	mka:MK0363.
NMPDR	fig|190192.1.peg.361.
Phylogenomic databases
HOGENOM	HBG391775.
OMA	QGTIAPD.
PhylomeDB	Q8TYD7.
ProtClustDB	PRK00919.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0363-MONOMER.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. IPR020536. ThiI_C. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00958. GMP_synt_C. 1 hit. PF02568. ThiI. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_METKA

Accession

Primary (citable) accession number: Q8TYD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program