Reviewed, UniProtKB/Swiss-Prot Q8TY76 (AROD_METKA)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate dehydratase
      Short name=3-dehydroquinase
    EC=4.2.1.10
Alternative name(s):
    Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	MK0429

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000138830
Sites
	Active site	114	1	Proton acceptor By similarity
	Active site	140	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TY76-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A92DAA620A48FB2B	FASTA	215	23,919
	10 20 30 40 50 60 MIIATLTGRT IEDMVELAIE AVEQGADALE VRLDYLENLD MSTALRAVRE CTRYERVVAT 70 80 90 100 110 120 LRREEEGGLY KGDEDRRLEI LERVSSEADY VDLELDVAEE EIISPSCETI VSYHNFENTP 130 140 150 160 170 180 PKEELIGIRD RCAELGDVAK VVTMARGHED ALRILEVVRT AEAPTIGFAM GEEAKYTRVV 190 200 210 SVLIGGFATY AAVRKKAAPG QLTVEETRKL LELLG

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01644.1.
RefSeq	NP_613714.1.
3D structure databases
SMR	Q8TY76. Positions 1-213.
ModBase	Search...
Genome annotation databases
GeneID	1477732.
GenomeReviews	Gene locus MK0429 in contig AE009439_GR.
KEGG	mka:MK0429.
NMPDR	fig|190192.1.peg.427.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG732926.
OMA	IAKLAVM.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0429-MONOMER.
BRENDA	4.2.1.10. 7577.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. aroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_METKA

Accession

Primary (citable) accession number: Q8TY76

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)