Q8TY09 (PURL_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Synonyms:	purL_2
Ordered Locus Names:	MK0497

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100514
Regions
	Nucleotide binding	91 – 102	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TY09 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A291CB5C84E7D331	FASTA	724	78,777
	10 20 30 40 50 60 MTLDERDLEL IREELGRDPN ATERAMFENM WSEHCAYRST RHLLRQLPSE ADHVIVGPGD 70 80 90 100 110 120 DAAVVAIDDE WAVVVGIESH NHPSYVDPYN GAATGVGGIV RDVLSMGAFP IALLDPLRFG 130 140 150 160 170 180 PLDGERVPYL VDGVVRGISD YGNRIGVPTV GGELEFDPSY ERNPLVNVMC VGIVRRSEIV 190 200 210 220 230 240 RGRADRPGDV LVLVGARTGR DGIGGAAFAS EELGEESEEE DRPAVQIGDP FTERQLIIAI 250 260 270 280 290 300 REAVERGLVK GCKDLGAAGL TCAATEMAAD GGTGVEIDVF KVPLREEGME PWEIMLSESQ 310 320 330 340 350 360 ERMLLVVAPE DVDEVIEICR KYGLEASVVG RVTDDGYLTV KDGDDVIARV PAEFLADGAP 370 380 390 400 410 420 EVEWEEEPYS YPENVDVPEP DPEDLVRSVL SSPNVSPALR EWVYRQYDHE VQGRTVVKPG 430 440 450 460 470 480 HDAAVMWLQH EGLEDVALAL TTDSNPRHVL IDPKTGTEGC VAEALRNLAT VGAEPLCLVD 490 500 510 520 530 540 CLNFGSPENP RVYYQLRRSI EGLGKAAREF EVPVVGGNVS LYNEHEVDGP VNPTPVIGAV 550 560 570 580 590 600 GVIRGLDYLE DFPREPEEGE AVIVLGETRE ELGGSLYLIE YHGIKGGKVP RVRYREERAL 610 620 630 640 650 660 HDLLRRIARK NMVSSVTDVS TGGLLAAVAE LLGPVGASLS LSEVPNSVSR WDFLLLSESH 670 680 690 700 710 720 GRAIVTTDRP DDVLGAAEEA GVPAQVVGEV TGDGVLRISV GPVDVSLDRE ELEELWRSPL HYLE

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01712.1.
RefSeq	NP_613782.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TY09.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1476598.
GenomeReviews	Gene locus MK0497 in contig AE009439_GR.
KEGG	mka:MK0497.
NMPDR	fig|190192.1.peg.495.
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q8TY09.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0497-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METKA

Accession

Primary (citable) accession number: Q8TY09

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program