Reviewed, UniProtKB/Swiss-Prot Q8TXY4 (MER_METKA)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydromethanopterin reductase
    EC=1.5.99.11
Alternative name(s):
    Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
    Methylene-H(4)MPT reductase

Gene names

Name:	mer
Ordered Locus Names:	MK0524

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT. HAMAP MF_01091
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_01091
Enzyme regulation	Requires the presence of relatively high concentrations of either sulfate or phosphate for maximal activity. HAMAP MF_01091
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP MF_01091
Subunit structure	Homotetramer composed of two loosely associated dimers Probable. HAMAP MF_01091
Subcellular location	Cytoplasm HAMAP MF_01091.
Sequence similarities	Belongs to the mer family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 349

348

5,10-methylenetetrahydromethanopterin reductase HAMAP MF_01091

PRO_0000084808

Experimental info

Sequence conflict

21

1

H → A AA sequence Ref.3

Sequence conflict

162

1

N → K in AAA92085. Ref.1

Sequence conflict

191

1

D → N in AAA92085. Ref.1

Sequence conflict

344

1

I → L in AAA92085. Ref.1

Secondary structure

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349

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Helix

15 – 27

13

Beta strand

32 – 35

4

Helix

44 – 53

10

Beta strand

56 – 65

10

Beta strand

67 – 70

4

Helix

72 – 85

14

Beta strand

92 – 94

3

Helix

99 – 105

7

Helix

126 – 141

16

Beta strand

151 – 154

4

Helix

167 – 169

3

Beta strand

172 – 175

4

Helix

179 – 188

10

Beta strand

190 – 195

6

Helix

199 – 215

17

Helix

220 – 222

3

Beta strand

223 – 228

6

Beta strand

231 – 233

3

Helix

237 – 253

17

Helix

257 – 262

6

Turn

269 – 271

3

Helix

272 – 276

5

Turn

277 – 280

4

Helix

282 – 288

7

Helix

291 – 297

7

Beta strand

299 – 302

4

Helix

303 – 315

13

Beta strand

320 – 323

4

Helix

331 – 341

11

Helix

343 – 345

3

Sequences

	Sequence		Length	Mass (Da)
	Q8TXY4-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 12AF28EDF65F26F0	FASTA	349	37,501
	10 20 30 40 50 60 MAEVSFGIEL LPDDKPTKIA HLIKVAEDNG FEYAWICDHY NNYSYMGVLT LAAVITSKIK 70 80 90 100 110 120 LGPGITNPYT RHPLITASNI ATLDWISGGR AIIGMGPGDK ATFDKMGLPF PCKIPIWNPE 130 140 150 160 170 180 AEDEVGPATA IREVKEVIYQ YLEGGPVEYE GKYVKTGTAD VNARSIQGSD IPFYMGAQGP 190 200 210 220 230 240 IMLKTAGEIA DGVLVNASNP KDFEVAVPKI EEGAKEAGRS LDEIDVAAYT CFSIDKDEDK 250 260 270 280 290 300 AIEATKIVVA FIVMGSPDVV LERHGIDTEK AEQIAEAIGK GDFGTAIGLV DEDMIEAFSI 310 320 330 340 AGDPDTVVDK IEELLKAGVT QVVVGSPIGP DKEKAIELVG QEVIPHFKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri." Noelling J., Pihl T.D., Reeve J.N. J. Bacteriol. 177:7238-7244(1995) [PubMed: 8522533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
[3]	"Purification and properties of N5,N10-methylenetetrahydromethanopterin reductase (coenzyme F420-dependent) from the extreme thermophile Methanopyrus kandleri." Ma K., Linder D., Stetter K.O., Thauer R.K. Arch. Microbiol. 155:593-600(1991) [PubMed: 1953299] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-27, CHARACTERIZATION. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.
[4]	"Structure of coenzyme F(420) dependent methylenetetrahydromethanopterin reductase from two methanogenic archaea." Shima S., Warkentin E., Grabarse W., Sordel M., Wicke M., Thauer R.K., Ermler U. J. Mol. Biol. 300:935-950(2000) [PubMed: 10891279] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS). Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U31567 Genomic DNA. Translation: AAA92085.1.
AE009439 Genomic DNA. Translation: AAM01739.1.

RefSeq

NP_613809.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EZW	X-ray	1.65	A	1-349	[»]

ModBase

Search...

Genome annotation databases

GeneID

1476625.

GenomeReviews

Gene locus MK0524 in contig AE009439_GR.

KEGG

mka:MK0524.

NMPDR

fig|190192.1.peg.522.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG497169.

OMA

LNEISDG.

Enzyme and pathway databases

BioCyc

MKAN190192:MK0524-MONOMER.

BRENDA

1.5.99.11. 7577.

Family and domain databases

HAMAP

MF_01091. F420_mer.
[Tree]

InterPro

IPR011251. Luciferase-like.
IPR019946. MeH4methanopterin_reductase.
[Graphical view]

Gene3D

G3DSA:3.20.20.30. Luciferase_like. 1 hit.

TIGRFAMs

TIGR03555. F420_mer. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MER_METKA

Accession

Primary (citable) accession number: Q8TXY4
Secondary accession number(s): Q49598

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)