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Q8TXW8

- AGOG_METKA

UniProt

Q8TXW8 - AGOG_METKA

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Protein

N-glycosylase/DNA lyase

Gene
MK0541
Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates By similarity.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 2518-oxoguanine By similarity
Binding sitei52 – 5218-oxoguanine; via carbonyl oxygen By similarity
Binding sitei63 – 6318-oxoguanine By similarity
Active sitei143 – 1431Schiff-base intermediate with DNA By similarity
Binding sitei147 – 14718-oxoguanine By similarity
Binding sitei173 – 17318-oxoguanine; via carbonyl oxygen By similarity
Active sitei175 – 1751 Reviewed prediction
Binding sitei209 – 20918-oxoguanine By similarity
Binding sitei213 – 21318-oxoguanine By similarity

GO - Molecular functioni

  1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA excision, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyase
Alternative name(s):
8-oxoguanine DNA glycosylase (EC:3.2.2.-)
AGOG
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Ordered Locus Names:MK0541
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 242242N-glycosylase/DNA lyaseUniRule annotationPRO_0000185110Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi190192.MK0541.

Structurei

3D structure databases

ProteinModelPortaliQ8TXW8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 18365Helix-hairpin-helixUniRule annotationAdd
BLAST

Domaini

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4047.
HOGENOMiHOG000254352.
KOiK01741.
OMAiVKMFGYA.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_01168. AGOG.
InterProiIPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view]
PfamiPF09171. DUF1886. 1 hit.
[Graphical view]
PIRSFiPIRSF008955. AGOG. 1 hit.
SUPFAMiSSF48150. SSF48150. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TXW8-1 [UniParc]FASTAAdd to Basket

« Hide

MTVPTFLRKV TLREICVIEE YVDVQYRAIE ALMDTLPSTD LVRLTVANAL    50
VSYQLSSSGE DWWREFSSYF RERRPRDIVR EYARFLPRSR GNRRLIRQKL 100
RRLHRAKAFL EELSWQDAKS YYRDMNRLRL DLARVLNADP ESKTIVFTVK 150
MFGYALRAIT GRFRPYPFEI PIPVDARIER ITRRITNDDP QLYWDSIARR 200
TGIPPLHLDS ILWVGTSRDP EVKRLLAKVL PKLIGELEML GN 242
Length:242
Mass (Da):28,614
Last modified:June 1, 2002 - v1
Checksum:i25FF075E3383719E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM01756.1.
RefSeqiNP_613826.1. NC_003551.1.
WP_011018911.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM01756; AAM01756; MK0541.
GeneIDi1476642.
KEGGimka:MK0541.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM01756.1 .
RefSeqi NP_613826.1. NC_003551.1.
WP_011018911.1. NC_003551.1.

3D structure databases

ProteinModelPortali Q8TXW8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190192.MK0541.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM01756 ; AAM01756 ; MK0541 .
GeneIDi 1476642.
KEGGi mka:MK0541.

Phylogenomic databases

eggNOGi COG4047.
HOGENOMi HOG000254352.
KOi K01741.
OMAi VKMFGYA.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_01168. AGOG.
InterProi IPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view ]
Pfami PF09171. DUF1886. 1 hit.
[Graphical view ]
PIRSFi PIRSF008955. AGOG. 1 hit.
SUPFAMi SSF48150. SSF48150. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiAGOG_METKA
AccessioniPrimary (citable) accession number: Q8TXW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3