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Q8TXW8 (AGOG_METKA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
N-glycosylase/DNA lyase
Alternative name(s):
8-oxoguanine DNA glycosylase
EC=3.2.2.-
AGOG
DNA-(apurinic or apyrimidinic site) lyase
Short name=AP lyase
EC=4.2.99.18
Gene names
Ordered Locus Names:MK0541
OrganismMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]
Taxonomic identifier190192 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus ›

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates By similarity. HAMAP-Rule MF_01168

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_01168

Domain

Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif). HAMAP-Rule MF_01168

Sequence similarities

Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 242242N-glycosylase/DNA lyase HAMAP-Rule MF_01168
PRO_0000185110

Regions

Region119 – 18365Helix-hairpin-helix HAMAP-Rule MF_01168

Sites

Active site1431Schiff-base intermediate with DNA By similarity
Active site1751 Potential
Binding site2518-oxoguanine By similarity
Binding site5218-oxoguanine; via carbonyl oxygen By similarity
Binding site6318-oxoguanine By similarity
Binding site14718-oxoguanine By similarity
Binding site17318-oxoguanine; via carbonyl oxygen By similarity
Binding site20918-oxoguanine By similarity
Binding site21318-oxoguanine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TXW8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 25FF075E3383719E

FASTA24228,614
        10         20         30         40         50         60 
MTVPTFLRKV TLREICVIEE YVDVQYRAIE ALMDTLPSTD LVRLTVANAL VSYQLSSSGE 

        70         80         90        100        110        120 
DWWREFSSYF RERRPRDIVR EYARFLPRSR GNRRLIRQKL RRLHRAKAFL EELSWQDAKS 

       130        140        150        160        170        180 
YYRDMNRLRL DLARVLNADP ESKTIVFTVK MFGYALRAIT GRFRPYPFEI PIPVDARIER 

       190        200        210        220        230        240 
ITRRITNDDP QLYWDSIARR TGIPPLHLDS ILWVGTSRDP EVKRLLAKVL PKLIGELEML 


GN

« Hide

References

[1]"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE009439 Genomic DNA. Translation: AAM01756.1.
RefSeqNP_613826.1. NC_003551.1.

3D structure databases

ProteinModelPortalQ8TXW8.
ModBaseSearch...

Protein-protein interaction databases

STRING190192.MK0541.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM01756; AAM01756; MK0541.
GeneID1476642.
KEGGmka:MK0541.

Phylogenomic databases

eggNOGCOG4047.
HOGENOMHOG000254352.
KOK01741.
OMAVKMFGYA.
ProtClustDBPRK13280.

Family and domain databases

Gene3D1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPMF_01168. AGOG.
InterProIPR011257. DNA_glycosylase.
IPR023170. HTH_base_excis_C.
IPR015254. N-Glyclase/DNA_lyase-like_arc.
IPR016544. N-Glyclase/DNA_lyase_arc.
[Graphical view]
PfamPF09171. DUF1886. 1 hit.
[Graphical view]
PIRSFPIRSF008955. AGOG. 1 hit.
SUPFAMSSF48150. DNA_glycsylse. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAGOG_METKA
AccessionPrimary (citable) accession number: Q8TXW8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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