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Q8TXW8

- AGOG_METKA

UniProt

Q8TXW8 - AGOG_METKA

Protein

N-glycosylase/DNA lyase

Gene

MK0541

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.UniRule annotation

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 2518-oxoguanineUniRule annotation
    Binding sitei52 – 5218-oxoguanine; via carbonyl oxygenUniRule annotation
    Binding sitei63 – 6318-oxoguanineUniRule annotation
    Active sitei143 – 1431Schiff-base intermediate with DNAUniRule annotation
    Binding sitei147 – 14718-oxoguanineUniRule annotation
    Binding sitei173 – 17318-oxoguanine; via carbonyl oxygenUniRule annotation
    Active sitei175 – 1751UniRule annotation
    Binding sitei209 – 20918-oxoguanineUniRule annotation
    Binding sitei213 – 21318-oxoguanineUniRule annotation

    GO - Molecular functioni

    1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
    2. oxidized base lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. base-excision repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA excision, DNA repair

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-glycosylase/DNA lyaseUniRule annotation
    Alternative name(s):
    8-oxoguanine DNA glycosylaseUniRule annotation (EC:3.2.2.-UniRule annotation)
    AGOGUniRule annotation
    DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation (EC:4.2.99.18UniRule annotation)
    Short name:
    AP lyaseUniRule annotation
    Gene namesi
    Ordered Locus Names:MK0541
    OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
    Taxonomic identifieri190192 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
    ProteomesiUP000001826: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 242242N-glycosylase/DNA lyasePRO_0000185110Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi190192.MK0541.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TXW8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni119 – 18365Helix-hairpin-helixAdd
    BLAST

    Domaini

    Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).

    Sequence similaritiesi

    Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG4047.
    HOGENOMiHOG000254352.
    KOiK01741.
    OMAiVKMFGYA.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPiMF_01168. AGOG.
    InterProiIPR011257. DNA_glycosylase.
    IPR023170. HTH_base_excis_C.
    IPR015254. N-Glyclase/DNA_lyase-like_arc.
    IPR016544. N-Glyclase/DNA_lyase_arc.
    [Graphical view]
    PfamiPF09171. DUF1886. 1 hit.
    [Graphical view]
    PIRSFiPIRSF008955. AGOG. 1 hit.
    SUPFAMiSSF48150. SSF48150. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8TXW8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVPTFLRKV TLREICVIEE YVDVQYRAIE ALMDTLPSTD LVRLTVANAL    50
    VSYQLSSSGE DWWREFSSYF RERRPRDIVR EYARFLPRSR GNRRLIRQKL 100
    RRLHRAKAFL EELSWQDAKS YYRDMNRLRL DLARVLNADP ESKTIVFTVK 150
    MFGYALRAIT GRFRPYPFEI PIPVDARIER ITRRITNDDP QLYWDSIARR 200
    TGIPPLHLDS ILWVGTSRDP EVKRLLAKVL PKLIGELEML GN 242
    Length:242
    Mass (Da):28,614
    Last modified:June 1, 2002 - v1
    Checksum:i25FF075E3383719E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009439 Genomic DNA. Translation: AAM01756.1.
    RefSeqiNP_613826.1. NC_003551.1.
    WP_011018911.1. NC_003551.1.

    Genome annotation databases

    EnsemblBacteriaiAAM01756; AAM01756; MK0541.
    GeneIDi1476642.
    KEGGimka:MK0541.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009439 Genomic DNA. Translation: AAM01756.1 .
    RefSeqi NP_613826.1. NC_003551.1.
    WP_011018911.1. NC_003551.1.

    3D structure databases

    ProteinModelPortali Q8TXW8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 190192.MK0541.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM01756 ; AAM01756 ; MK0541 .
    GeneIDi 1476642.
    KEGGi mka:MK0541.

    Phylogenomic databases

    eggNOGi COG4047.
    HOGENOMi HOG000254352.
    KOi K01741.
    OMAi VKMFGYA.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    HAMAPi MF_01168. AGOG.
    InterProi IPR011257. DNA_glycosylase.
    IPR023170. HTH_base_excis_C.
    IPR015254. N-Glyclase/DNA_lyase-like_arc.
    IPR016544. N-Glyclase/DNA_lyase_arc.
    [Graphical view ]
    Pfami PF09171. DUF1886. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF008955. AGOG. 1 hit.
    SUPFAMi SSF48150. SSF48150. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

    Entry informationi

    Entry nameiAGOG_METKA
    AccessioniPrimary (citable) accession number: Q8TXW8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3