Reviewed, UniProtKB/Swiss-Prot Q8TXV8 (GUAAA_METKA)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit A
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase

Gene names

Name:	guaAA
Synonyms:	guaA_2
Ordered Locus Names:	MK0551

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140222
Regions
	Domain	1 – 187	187	Glutamine amidotransferase type-1
Sites
	Active site	76	1	Nucleophile By similarity
	Active site	164	1	By similarity
	Active site	166	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TXV8-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A76FEDA8E3D7B304	FASTA	187	21,042
	10 20 30 40 50 60 MILIIDNHGQ YVHLIRKNFD YMGVPAEIIP NTTDPEDVRE RASGVVISGG PSRERAGNSR 70 80 90 100 110 120 EIIEELTGEV PILGICLGHQ LMAEVFGGKV DWAAGREEYA RTEVEILDHE GIFEGLPDKI 130 140 150 160 170 180 VAWASHRDEV KEVPDEFVVT ARSDRCEVEA MRHEELPLYG VQFHPELKFT EYGPDILKNF AKLCGEL

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009439 Genomic DNA. Translation: AAM01766.1.
RefSeq	NP_613836.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
ModBase	Search...
Genome annotation databases
GeneID	1476652.
GenomeReviews	Gene locus MK0551 in contig AE009439_GR.
KEGG	mka:MK0551.
NMPDR	fig|190192.1.peg.549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TXV8.
OMA	GQYVHRI.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0551-MON.
BRENDA	6.3.5.2. 7577.
Family and domain databases
HAMAP	MF_01510. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR004739. GMP_synth_N. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_METKA

Accession

Primary (citable) accession number: Q8TXV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)