Reviewed, UniProtKB/Swiss-Prot Q8TXT8 (HDRB_METKA)

Names and origin

Protein names

Recommended name:
    CoB--CoM heterodisulfide reductase subunit B
    EC=1.8.98.1

Gene names

Name:	hdrB
Ordered Locus Names:	MK0572

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). HdrB may have a function in energy transduction By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Pathway	Cofactor metabolism; coenzyme B/coenzyme M regeneration; coenzyme B and coenzyme M from CoB-CoM heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase is composed of three subunits; hdrA, hdrB and hdrC By similarity.
Sequence similarities	Belongs to the hdrB family.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cofactor metabolic process Inferred from electronic annotation. Source: InterPro methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	CoB--CoM heterodisulfide reductase subunit B		PRO_0000150067

Sequences

	Sequence		Length	Mass (Da)
	Q8TXT8-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A23028DA7A976AAA	FASTA	305	34,131
	10 20 30 40 50 60 MAKKLCFFLG CIMPNRYPGI EKATRLVFEE LGYELVDMDG ASCCPAPGVF GSFDLKTWVT 70 80 90 100 110 120 IAARNLSIAE EKGYDILTVC NGCFGSLNEA NHLLQENPEL REFVNEKLAE IDREYKGKVK 130 140 150 160 170 180 VYHVNTFLYE EVGVKKIKEK VERPLEKTDG EPLKVAVHYG CHLLKPSEVT GFPGSVEDPR 190 200 210 220 230 240 TLDELVEALG AESVDYKDKI MCCGAGGGVR SRELKVSLHF TREKIFNMLE AGADCTTNVC 250 260 270 280 290 300 PFCHLQFDRG QIEMKEHFEK LPPKKLPVFH YCQLAGLAFG MDPEELALET HEIDCTPVLE KLGLA

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010351 Genomic DNA. Translation: AAM01787.1.
RefSeq	NP_613857.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1476673.
GenomeReviews	Gene locus MK0572 in contig AE009439_GR.
KEGG	mka:MK0572.
NMPDR	fig|190192.1.peg.570.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TXT8.
OMA	Q8TXT8. DKMMCCG.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0572-MON.
BRENDA	1.8.98.1. 7577.
Family and domain databases
InterPro	IPR017678. CoB/CoM_hetero-S_Rdtase_bsu. IPR004017. Cys_rich_region. [Graphical view]
Pfam	PF02754. CCG. 2 hits. [Graphical view]
TIGRFAMs	TIGR03288. CoB_CoM_SS_B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HDRB_METKA

Accession

Primary (citable) accession number: Q8TXT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)