Reviewed, UniProtKB/Swiss-Prot Q8TXT7 (HDRC_METKA)

Names and origin

Protein names

Recommended name:
    CoB--CoM heterodisulfide reductase iron-sulfur subunit C
    EC=1.8.98.1

Gene names

Name:	hdrC
Ordered Locus Names:	MK0573

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B) By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Pathway	Cofactor metabolism; coenzyme B/coenzyme M regeneration; coenzyme B and coenzyme M from CoB-CoM heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase is composed of three subunits; hdrA, hdrB and hdrC By similarity.
Sequence similarities	Belongs to the hdrC family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cofactor metabolic process Inferred from electronic annotation. Source: InterPro methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	CoB--CoM heterodisulfide reductase iron-sulfur subunit C		PRO_0000150075
Regions
	Domain	32 – 61	30	4Fe-4S ferredoxin-type 1
	Domain	75 – 105	31	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	41	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	44	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	47	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	51	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	85	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	88	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	91	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	95	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TXT7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2645D1648F85F4DB	FASTA	192	22,022
	10 20 30 40 50 60 MVEPRDTVIR EEDLNPDFLE ELSELVEPVF EEEEVLSVQA CYQCGTCTGS CPSGRRTSYR 70 80 90 100 110 120 TRLIMRKLQL GLVDEVIKSD ELWMCTTCYT CYERCPRGVK IVDAVKAARN LAAKKGYMAK 130 140 150 160 170 180 AHRMVAMFVI KTGHAVPIND EIREVRKNIG LDEVPPTTHR YEEALEEVQK LVKINEFDKL 190 IGYDWEEGDL VD

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010351 Genomic DNA. Translation: AAM01788.1.
RefSeq	NP_613858.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1476674.
GenomeReviews	Gene locus MK0573 in contig AE009439_GR.
KEGG	mka:MK0573.
NMPDR	fig|190192.1.peg.571.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TXT7.
OMA	Q8TXT7. GLNTRRI.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0573-MON.
BRENDA	1.8.98.1. 7577.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR017680. CoB/CoM_hetero-S_Rdtase_csu. IPR012285. Fum_reductase_C. [Graphical view]
Gene3D	G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR03290. CoB_CoM_SS_C. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HDRC_METKA

Accession

Primary (citable) accession number: Q8TXT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)