Q8TXN4 (AROA_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	MK0628

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Probable 3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088330

Sequences

	Sequence		Length	Mass (Da)
	Q8TXN4 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: CD8C67E906E935BD	FASTA	428	45,827
	10 20 30 40 50 60 MKRVELEGIP EVRGTVCPPP SKSGSHRALI AASLCDGSTE LWNVLDAEDV RATLRLCRML 70 80 90 100 110 120 GAEVDVDGEE RLEATVSGFG DSPRAPEDVV DCGNSGTTLR LGCGLAALVE GTTILTGDDS 130 140 150 160 170 180 LRSRPVGDLL AALRSLGVDA RGRVVRGEEY PPVVISGRPL RERVAVYGDV SSQFVSALLF 190 200 210 220 230 240 LGAGLGALRV DVVGDLRSRP YVDMTVETLE RFGVSVVREG SSFEVEGRPR SPGKLRVEND 250 260 270 280 290 300 WSSAGYFVAL GAIGGEMRIE GVDLDSSHPD RRIVEITREM GAEVRRIDGG IVVRSTGRLE 310 320 330 340 350 360 GVEVDLSDSP DLVPTVAAMA CFAEGVTRIE NVGHLRYKEV DRLRALAAEL PKFGVEVREG 370 380 390 400 410 420 KDWLEIVGGE PVGARVDSRG DHRMAMALAV VGAFARGKTV VERADAVSIS YPRFWEDLAS VGVPVHSV

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01843.1.
RefSeq	NP_613913.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TXN4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1476729.
GenomeReviews	Gene locus MK0628 in contig AE009439_GR.
KEGG	mka:MK0628.
NMPDR	fig|190192.1.peg.626.
Phylogenomic databases
HOGENOM	HBG646626.
OMA	GADIEWG.
PhylomeDB	Q8TXN4.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0628-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. False negative. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_METKA

Accession

Primary (citable) accession number: Q8TXN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program