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Q8TXK0

- SPCS_METKA

UniProt

Q8TXK0 - SPCS_METKA

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Protein

O-phosphoseryl-tRNA(Sec) selenium transferase

Gene

spcS

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.By similarity

Catalytic activityi

O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H2O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei66 – 661May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylateBy similarity
Binding sitei67 – 671PLPBy similarity
Binding sitei89 – 891SubstrateBy similarity
Binding sitei90 – 901SubstrateBy similarity
Binding sitei97 – 971SubstrateBy similarity
Binding sitei298 – 2981SubstrateBy similarity

GO - Molecular functioni

  1. pyridoxal phosphate binding Source: InterPro
  2. transferase activity, transferring selenium-containing groups Source: InterPro
  3. tRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
  2. translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Pyridoxal phosphate, RNA-binding, Selenium, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00906; UER00898.

Names & Taxonomyi

Protein namesi
Recommended name:
O-phosphoseryl-tRNA(Sec) selenium transferase (EC:2.9.1.2)
Alternative name(s):
Selenocysteine synthase
Short name:
Sec synthase
Selenocysteinyl-tRNA(Sec) synthase
Sep-tRNA:Sec-tRNA synthase
Short name:
SepSecS
Gene namesi
Name:spcS
Ordered Locus Names:MK0672
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431O-phosphoseryl-tRNA(Sec) selenium transferasePRO_0000219882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei269 – 2691N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi190192.MK0672.

Structurei

3D structure databases

ProteinModelPortaliQ8TXK0.
SMRiQ8TXK0. Positions 1-426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3636TetramerizationBy similarityAdd
BLAST
Regioni88 – 9811Phosphate loop (P-loop)By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the SepSecS family.Curated

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000254245.
KOiK03341.
OMAiHIINGAY.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR019872. Sec-tRNA_Se_transferase.
IPR008829. SLA/LP_auto_ag.
[Graphical view]
PANTHERiPTHR12944. PTHR12944. 1 hit.
PfamiPF05889. SLA_LP_auto_ag. 1 hit.
[Graphical view]
PIRSFiPIRSF017689. SepSecS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR03531. selenium_SpcS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TXK0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRGLIPDHML ERGRTVLDSY REPVERLLSE RRMPEEGWPD DVIATFLWEL
60 70 80 90 100
SRMDTDKDPK AARIGEREAR VASRLAEESV FGFCHGVGRS GTLVDPQPKA
110 120 130 140 150
PGASIMYALT NRLVTDFLRR LGFRIEGAFV VPGATGLSIA LCLSALGEGE
160 170 180 190 200
EVIYPYAAHK SPIKAVRLAG FGMRVVDTEI EGDRIVVDPG DVEEALERSE
210 220 230 240 250
SPAAVLSTLT FFPPRSSDPL PEIAELCEEY GVPHVVNAAY GIQHEQYRDL
260 270 280 290 300
LNRAIKRGRV DVVVSSTDKN LLTPVGGGIV YAPDEETLRE VSRAYPGRAS
310 320 330 340 350
AAPVAHALIS LLSLGMKGYR RLMRRQKECK ALLDELLEDL EARRDDVRVL
360 370 380 390 400
DVDNPIASAV AVEGHDPVDL AARLYVRRVT GPRGVRADDP FGTSRLRGYH
410 420 430
SNYITINAAI GVREEDVKTA VERLERELEG E
Length:431
Mass (Da):47,589
Last modified:June 1, 2002 - v1
Checksum:i4557FD9AF640CA03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM01887.1.
RefSeqiNP_613957.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM01887; AAM01887; MK0672.
GeneIDi1476773.
KEGGimka:MK0672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM01887.1 .
RefSeqi NP_613957.1. NC_003551.1.

3D structure databases

ProteinModelPortali Q8TXK0.
SMRi Q8TXK0. Positions 1-426.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190192.MK0672.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM01887 ; AAM01887 ; MK0672 .
GeneIDi 1476773.
KEGGi mka:MK0672.

Phylogenomic databases

eggNOGi COG0076.
HOGENOMi HOG000254245.
KOi K03341.
OMAi HIINGAY.

Enzyme and pathway databases

UniPathwayi UPA00906 ; UER00898 .

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR019872. Sec-tRNA_Se_transferase.
IPR008829. SLA/LP_auto_ag.
[Graphical view ]
PANTHERi PTHR12944. PTHR12944. 1 hit.
Pfami PF05889. SLA_LP_auto_ag. 1 hit.
[Graphical view ]
PIRSFi PIRSF017689. SepSecS. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR03531. selenium_SpcS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiSPCS_METKA
AccessioniPrimary (citable) accession number: Q8TXK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2002
Last modified: November 26, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3