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Q8TXK0

- SPCS_METKA

UniProt

Q8TXK0 - SPCS_METKA

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Protein

O-phosphoseryl-tRNA(Sec) selenium transferase

Gene
spcS, MK0672
Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis By similarity.

Catalytic activityi

O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H2O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate.

Cofactori

Pyridoxal phosphate By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei66 – 661May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylate By similarity
Binding sitei67 – 671PLP By similarity
Binding sitei89 – 891Substrate By similarity
Binding sitei90 – 901Substrate By similarity
Binding sitei97 – 971Substrate By similarity
Binding sitei298 – 2981Substrate By similarity

GO - Molecular functioni

  1. pyridoxal phosphate binding Source: InterPro
  2. transferase activity, transferring selenium-containing groups Source: InterPro
  3. tRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
  2. translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Pyridoxal phosphate, RNA-binding, Selenium, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00906; UER00898.

Names & Taxonomyi

Protein namesi
Recommended name:
O-phosphoseryl-tRNA(Sec) selenium transferase (EC:2.9.1.2)
Alternative name(s):
Selenocysteine synthase
Short name:
Sec synthase
Selenocysteinyl-tRNA(Sec) synthase
Sep-tRNA:Sec-tRNA synthase
Short name:
SepSecS
Gene namesi
Name:spcS
Ordered Locus Names:MK0672
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431O-phosphoseryl-tRNA(Sec) selenium transferasePRO_0000219882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei269 – 2691N6-(pyridoxal phosphate)lysine By similarity

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi190192.MK0672.

Structurei

3D structure databases

ProteinModelPortaliQ8TXK0.
SMRiQ8TXK0. Positions 1-426.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3636Tetramerization By similarityAdd
BLAST
Regioni88 – 9811Phosphate loop (P-loop) By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the SepSecS family.

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000254245.
KOiK03341.
OMAiHIINGAY.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR019872. Sec-tRNA_Se_transferase.
IPR008829. SLA/LP_auto_ag.
[Graphical view]
PANTHERiPTHR12944. PTHR12944. 1 hit.
PfamiPF05889. SLA_LP_auto_ag. 1 hit.
[Graphical view]
PIRSFiPIRSF017689. SepSecS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR03531. selenium_SpcS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TXK0-1 [UniParc]FASTAAdd to Basket

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MRGLIPDHML ERGRTVLDSY REPVERLLSE RRMPEEGWPD DVIATFLWEL    50
SRMDTDKDPK AARIGEREAR VASRLAEESV FGFCHGVGRS GTLVDPQPKA 100
PGASIMYALT NRLVTDFLRR LGFRIEGAFV VPGATGLSIA LCLSALGEGE 150
EVIYPYAAHK SPIKAVRLAG FGMRVVDTEI EGDRIVVDPG DVEEALERSE 200
SPAAVLSTLT FFPPRSSDPL PEIAELCEEY GVPHVVNAAY GIQHEQYRDL 250
LNRAIKRGRV DVVVSSTDKN LLTPVGGGIV YAPDEETLRE VSRAYPGRAS 300
AAPVAHALIS LLSLGMKGYR RLMRRQKECK ALLDELLEDL EARRDDVRVL 350
DVDNPIASAV AVEGHDPVDL AARLYVRRVT GPRGVRADDP FGTSRLRGYH 400
SNYITINAAI GVREEDVKTA VERLERELEG E 431
Length:431
Mass (Da):47,589
Last modified:June 1, 2002 - v1
Checksum:i4557FD9AF640CA03
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM01887.1.
RefSeqiNP_613957.1. NC_003551.1.
WP_011019042.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM01887; AAM01887; MK0672.
GeneIDi1476773.
KEGGimka:MK0672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM01887.1 .
RefSeqi NP_613957.1. NC_003551.1.
WP_011019042.1. NC_003551.1.

3D structure databases

ProteinModelPortali Q8TXK0.
SMRi Q8TXK0. Positions 1-426.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190192.MK0672.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM01887 ; AAM01887 ; MK0672 .
GeneIDi 1476773.
KEGGi mka:MK0672.

Phylogenomic databases

eggNOGi COG0076.
HOGENOMi HOG000254245.
KOi K03341.
OMAi HIINGAY.

Enzyme and pathway databases

UniPathwayi UPA00906 ; UER00898 .

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
InterProi IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR019872. Sec-tRNA_Se_transferase.
IPR008829. SLA/LP_auto_ag.
[Graphical view ]
PANTHERi PTHR12944. PTHR12944. 1 hit.
Pfami PF05889. SLA_LP_auto_ag. 1 hit.
[Graphical view ]
PIRSFi PIRSF017689. SepSecS. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR03531. selenium_SpcS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiSPCS_METKA
AccessioniPrimary (citable) accession number: Q8TXK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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