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Q8TXK0

- SPCS_METKA

UniProt

Q8TXK0 - SPCS_METKA

Protein

O-phosphoseryl-tRNA(Sec) selenium transferase

Gene

spcS

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.By similarity

    Catalytic activityi

    O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H2O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei66 – 661May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylateBy similarity
    Binding sitei67 – 671PLPBy similarity
    Binding sitei89 – 891SubstrateBy similarity
    Binding sitei90 – 901SubstrateBy similarity
    Binding sitei97 – 971SubstrateBy similarity
    Binding sitei298 – 2981SubstrateBy similarity

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. transferase activity, transferring selenium-containing groups Source: InterPro
    3. tRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
    2. translation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate, RNA-binding, Selenium, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00906; UER00898.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    O-phosphoseryl-tRNA(Sec) selenium transferase (EC:2.9.1.2)
    Alternative name(s):
    Selenocysteine synthase
    Short name:
    Sec synthase
    Selenocysteinyl-tRNA(Sec) synthase
    Sep-tRNA:Sec-tRNA synthase
    Short name:
    SepSecS
    Gene namesi
    Name:spcS
    Ordered Locus Names:MK0672
    OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
    Taxonomic identifieri190192 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
    ProteomesiUP000001826: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 431431O-phosphoseryl-tRNA(Sec) selenium transferasePRO_0000219882Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei269 – 2691N6-(pyridoxal phosphate)lysineBy similarity

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi190192.MK0672.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TXK0.
    SMRiQ8TXK0. Positions 1-426.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 3636TetramerizationBy similarityAdd
    BLAST
    Regioni88 – 9811Phosphate loop (P-loop)By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SepSecS family.Curated

    Phylogenomic databases

    eggNOGiCOG0076.
    HOGENOMiHOG000254245.
    KOiK03341.
    OMAiHIINGAY.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    InterProiIPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR019872. Sec-tRNA_Se_transferase.
    IPR008829. SLA/LP_auto_ag.
    [Graphical view]
    PANTHERiPTHR12944. PTHR12944. 1 hit.
    PfamiPF05889. SLA_LP_auto_ag. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017689. SepSecS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR03531. selenium_SpcS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8TXK0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRGLIPDHML ERGRTVLDSY REPVERLLSE RRMPEEGWPD DVIATFLWEL    50
    SRMDTDKDPK AARIGEREAR VASRLAEESV FGFCHGVGRS GTLVDPQPKA 100
    PGASIMYALT NRLVTDFLRR LGFRIEGAFV VPGATGLSIA LCLSALGEGE 150
    EVIYPYAAHK SPIKAVRLAG FGMRVVDTEI EGDRIVVDPG DVEEALERSE 200
    SPAAVLSTLT FFPPRSSDPL PEIAELCEEY GVPHVVNAAY GIQHEQYRDL 250
    LNRAIKRGRV DVVVSSTDKN LLTPVGGGIV YAPDEETLRE VSRAYPGRAS 300
    AAPVAHALIS LLSLGMKGYR RLMRRQKECK ALLDELLEDL EARRDDVRVL 350
    DVDNPIASAV AVEGHDPVDL AARLYVRRVT GPRGVRADDP FGTSRLRGYH 400
    SNYITINAAI GVREEDVKTA VERLERELEG E 431
    Length:431
    Mass (Da):47,589
    Last modified:June 1, 2002 - v1
    Checksum:i4557FD9AF640CA03
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009439 Genomic DNA. Translation: AAM01887.1.
    RefSeqiNP_613957.1. NC_003551.1.
    WP_011019042.1. NC_003551.1.

    Genome annotation databases

    EnsemblBacteriaiAAM01887; AAM01887; MK0672.
    GeneIDi1476773.
    KEGGimka:MK0672.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009439 Genomic DNA. Translation: AAM01887.1 .
    RefSeqi NP_613957.1. NC_003551.1.
    WP_011019042.1. NC_003551.1.

    3D structure databases

    ProteinModelPortali Q8TXK0.
    SMRi Q8TXK0. Positions 1-426.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 190192.MK0672.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM01887 ; AAM01887 ; MK0672 .
    GeneIDi 1476773.
    KEGGi mka:MK0672.

    Phylogenomic databases

    eggNOGi COG0076.
    HOGENOMi HOG000254245.
    KOi K03341.
    OMAi HIINGAY.

    Enzyme and pathway databases

    UniPathwayi UPA00906 ; UER00898 .

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    InterProi IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR019872. Sec-tRNA_Se_transferase.
    IPR008829. SLA/LP_auto_ag.
    [Graphical view ]
    PANTHERi PTHR12944. PTHR12944. 1 hit.
    Pfami PF05889. SLA_LP_auto_ag. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017689. SepSecS. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR03531. selenium_SpcS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

    Entry informationi

    Entry nameiSPCS_METKA
    AccessioniPrimary (citable) accession number: Q8TXK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3