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Protein

O-phosphoseryl-tRNA(Sec) selenium transferase

Gene

spcS

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.By similarity

Catalytic activityi

O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H2O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate.By similarity

Cofactori

pyridoxal 5'-phosphateBy similarity

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route).By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-seryl-tRNA(Sec) kinase (pstK)
  2. O-phosphoseryl-tRNA(Sec) selenium transferase (spcS)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei66May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylateBy similarity1
Binding sitei67Pyridoxal phosphateBy similarity1
Binding sitei89SubstrateBy similarity1
Binding sitei90SubstrateBy similarity1
Binding sitei97SubstrateBy similarity1
Binding sitei298SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processProtein biosynthesis
LigandPyridoxal phosphate, Selenium

Enzyme and pathway databases

UniPathwayiUPA00906; UER00898

Names & Taxonomyi

Protein namesi
Recommended name:
O-phosphoseryl-tRNA(Sec) selenium transferase (EC:2.9.1.2By similarity)
Alternative name(s):
Selenocysteine synthase
Short name:
Sec synthase
Selenocysteinyl-tRNA(Sec) synthase
Sep-tRNA:Sec-tRNA synthase
Short name:
SepSecS
Gene namesi
Name:spcS
Ordered Locus Names:MK0672
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002198821 – 431O-phosphoseryl-tRNA(Sec) selenium transferaseAdd BLAST431

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei269N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi190192.MK0672

Structurei

3D structure databases

ProteinModelPortaliQ8TXK0
SMRiQ8TXK0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 36TetramerizationBy similarityAdd BLAST36
Regioni88 – 98Phosphate loop (P-loop)By similarityAdd BLAST11

Sequence similaritiesi

Belongs to the SepSecS family.Curated

Phylogenomic databases

eggNOGiarCOG00119 Archaea
COG0076 LUCA
HOGENOMiHOG000254245
KOiK03341
OMAiHIINGAY
OrthoDBiPOG093Z026N

Family and domain databases

Gene3Di3.40.640.10, 1 hit
InterProiView protein in InterPro
IPR015424 PyrdxlP-dep_Trfase
IPR015421 PyrdxlP-dep_Trfase_major
IPR019872 Sec-tRNA_Se_transferase
IPR008829 SepSecS/SepCysS
PANTHERiPTHR12944 PTHR12944, 1 hit
PfamiView protein in Pfam
PF05889 SepSecS, 1 hit
PIRSFiPIRSF017689 SepSecS, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR03531 selenium_SpcS, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TXK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGLIPDHML ERGRTVLDSY REPVERLLSE RRMPEEGWPD DVIATFLWEL
60 70 80 90 100
SRMDTDKDPK AARIGEREAR VASRLAEESV FGFCHGVGRS GTLVDPQPKA
110 120 130 140 150
PGASIMYALT NRLVTDFLRR LGFRIEGAFV VPGATGLSIA LCLSALGEGE
160 170 180 190 200
EVIYPYAAHK SPIKAVRLAG FGMRVVDTEI EGDRIVVDPG DVEEALERSE
210 220 230 240 250
SPAAVLSTLT FFPPRSSDPL PEIAELCEEY GVPHVVNAAY GIQHEQYRDL
260 270 280 290 300
LNRAIKRGRV DVVVSSTDKN LLTPVGGGIV YAPDEETLRE VSRAYPGRAS
310 320 330 340 350
AAPVAHALIS LLSLGMKGYR RLMRRQKECK ALLDELLEDL EARRDDVRVL
360 370 380 390 400
DVDNPIASAV AVEGHDPVDL AARLYVRRVT GPRGVRADDP FGTSRLRGYH
410 420 430
SNYITINAAI GVREEDVKTA VERLERELEG E
Length:431
Mass (Da):47,589
Last modified:June 1, 2002 - v1
Checksum:i4557FD9AF640CA03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA Translation: AAM01887.1

Genome annotation databases

EnsemblBacteriaiAAM01887; AAM01887; MK0672
KEGGimka:MK0672
PATRICifig|190192.8.peg.710

Similar proteinsi

Entry informationi

Entry nameiSPCS_METKA
AccessioniPrimary (citable) accession number: Q8TXK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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