Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8TXJ4

- EF2_METKA

UniProt

Q8TXJ4 - EF2_METKA

Protein

Elongation factor 2

Gene

fusA

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi616 – 6205GTPUniRule annotation
    Nucleotide bindingi670 – 6734GTPUniRule annotation

    GO - Molecular functioni

    1. endonuclease activity Source: UniProtKB-KW
    2. GTPase activity Source: InterPro
    3. GTP binding Source: UniProtKB-KW
    4. translation elongation factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro
    2. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Elongation factor, Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Intron homing, Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor 2UniRule annotation
    Short name:
    EF-2UniRule annotation
    Cleaved into the following chain:
    Gene namesi
    Name:fusAUniRule annotation
    Ordered Locus Names:MK0679
    OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
    Taxonomic identifieri190192 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
    ProteomesiUP000001826: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3434Elongation factor 2, 1st partSequence AnalysisPRO_0000007436Add
    BLAST
    Chaini35 – 556522Mka FusA inteinSequence AnalysisPRO_0000007437Add
    BLAST
    Chaini557 – 1257701Elongation factor 2, 2nd partSequence AnalysisPRO_0000007438Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1120 – 11201DiphthamideUniRule annotation

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.By similarity

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Protein-protein interaction databases

    STRINGi190192.MK0679.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TXJ4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini273 – 402130DOD-type homing endonucleaseAdd
    BLAST
    Domaini541 – 782242tr-type GAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0480.
    HOGENOMiHOG000112334.
    KOiK03234.
    OMAiHIDHGKC.

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 3 hits.
    HAMAPiMF_00054_A. EF_G_EF_2_A.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR004543. Transl_elong_EFG/EF2_arc.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF14492. EFG_II. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00838. EFG_C. 1 hit.
    SM00889. EFG_IV. 1 hit.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR00490. aEF-2. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8TXJ4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRKEKMAEK CRKLMTEPGK IRNIGIIAHI DHGKCVAPET KICLADGRFV     50
    RADELFEELK ERGRLVKCDE SEEVYELREP VGVSSLDKDA VEIVEGKITH 100
    VWRLKADKLV EVEVKNGRSI RTTPEHKFLV LDPSGEIVEK RADELEIGDY 150
    IVCTQKLVHE GMSEEELKRE VFRRLGRDFF VHLPEEEAES VLELAKERGI 200
    KALWETLEVD IEENSFYYQL RKGRIRADIL VDLAEELGLD LADLYDAVEV 250
    SYRSNTKSTK PIRLPEPEDL FYLAGLMFGD GCWNQLTNGS EAIQGEVKRI 300
    ASDMGLEVRV RRYEGKTARI DFPETVPRIL EALFDYPRRK KAHRIRVNDF 350
    LTRAPLDCIA EFIRGYFDAD GTVEEGRSAV SVTSVSREFL EDLQLLLQKF 400
    DVASYLREGD GAYTLYVSGA RSLERFPGFR EPEKAEKLKK LMEKASSSEL 450
    EKVPISGEIL REVRGDVPTT RMFNCYSNYE GGQVGLTKSS LEKVISTLEA 500
    VGVEGEALER LKALARDDVC FLEVVRVEEV EYDGYVYDFT VEEHHNFAAE 550
    GFVVHNTTLS DQLLAGAGMI SEELAGDQLV LDFDEMEQER GITIDAANVS 600
    MVHEYEGEEY LINLIDTPGH VDFSGDVTRA MRAVDGAIVV VCAVEGVMPQ 650
    TETVLRQALR ERVRPVLYIN KVDRLINELK LSPEEMQNRF LEIISEVNKM 700
    IEQMAPEEFK DEWKVSVEDG SVAFGSAYYG WGISFPFMEK TGITFKDIIE 750
    YCQQDKQKEL AQEAPVYQVV LDMVVKHLPD PVTAQEYRIE QIWPGDPESE 800
    DGKTLRKCDP NGKLAMVVTD VRIDEHAGEV ATGRVYSGTI REGQQVYLAS 850
    SKKETRVQQV GIYMGPDRIR TDEVPAGNIA AVTGLRDVWA GETVTDPEDP 900
    IEPFEELQHF AEPVVTVAVE AKNTQDLPKL IEILHQIAKE DPTVKVEINE 950
    ETGQHLVSGM GELHLEIIAH RIKERGVDIK VSEPIVVYRE GVFGVCDDEV 1000
    EGKSPNKHNK FYVTVEPVEE EIVEAIEEGK FNPEEMSKKE LEETLMEYGM 1050
    DRDDAKAVET VKGTNFFLDK TVGLQYLNEV MELLIEGFEE AMEEGPLAKE 1100
    PCRGVKVSLV DAEIHEDPVH RGPAQVIPAI KRAIYGGMLL ADTHLLEPMQ 1150
    YIYVTVPQDY MGAVTKEIQG RRGTIEEIQQ EGDTVIIKGK APVAEMFGFA 1200
    NDIRSATEGR AIWTTEHAGY ERVPEELEEQ IIREIRERKG LKPEPPKPED 1250
    YIEDYGG 1257
    Length:1,257
    Mass (Da):142,071
    Last modified:June 1, 2002 - v1
    Checksum:i2C916583EF9D9B3C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009439 Genomic DNA. Translation: AAM01894.1.
    RefSeqiNP_613964.1. NC_003551.1.

    Genome annotation databases

    EnsemblBacteriaiAAM01894; AAM01894; MK0679.
    GeneIDi1476780.
    KEGGimka:MK0679.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009439 Genomic DNA. Translation: AAM01894.1 .
    RefSeqi NP_613964.1. NC_003551.1.

    3D structure databases

    ProteinModelPortali Q8TXJ4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 190192.MK0679.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM01894 ; AAM01894 ; MK0679 .
    GeneIDi 1476780.
    KEGGi mka:MK0679.

    Phylogenomic databases

    eggNOGi COG0480.
    HOGENOMi HOG000112334.
    KOi K03234.
    OMAi HIDHGKC.

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.30.230.10. 1 hit.
    3.30.70.240. 1 hit.
    3.40.50.300. 3 hits.
    HAMAPi MF_00054_A. EF_G_EF_2_A.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR004543. Transl_elong_EFG/EF2_arc.
    IPR005517. Transl_elong_EFG/EF2_IV.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF14492. EFG_II. 1 hit.
    PF03764. EFG_IV. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00838. EFG_C. 1 hit.
    SM00889. EFG_IV. 1 hit.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF54980. SSF54980. 2 hits.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR00490. aEF-2. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

    Entry informationi

    Entry nameiEF2_METKA
    AccessioniPrimary (citable) accession number: Q8TXJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 29, 2003
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The intein interrupts the GTP-binding site.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3