Elongation factor 2 - Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Elongation factor 2
Short name=EF-2

Cleaved into the following chain:

Mka fusA intein

Gene names

Name:	fusA
Ordered Locus Names:	MK0679

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	1257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_A.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity. HAMAP MF_00054_A
Miscellaneous	The intein interrupts the GTP-binding site. HAMAP MF_00054_A
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 34

34

Elongation factor 2, 1st part Potential

PRO_0000007436

Chain

35 – 556

522

Mka fusA intein Potential

PRO_0000007437

Chain

557 – 1257

701

Elongation factor 2, 2nd part Potential

PRO_0000007438

Regions

Domain

273 – 402

130

DOD-type homing endonuclease

Nucleotide binding

616 – 620

5

GTP By similarity

Nucleotide binding

670 – 673

4

GTP By similarity

Amino acid modifications

Modified residue

1120

1

Diphthamide By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8TXJ4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 2C916583EF9D9B3C
Show »

FASTA

1,257

142,071

        10         20         30         40         50         60 
MGRKEKMAEK CRKLMTEPGK IRNIGIIAHI DHGKCVAPET KICLADGRFV RADELFEELK 

        70         80         90        100        110        120 
ERGRLVKCDE SEEVYELREP VGVSSLDKDA VEIVEGKITH VWRLKADKLV EVEVKNGRSI 

       130        140        150        160        170        180 
RTTPEHKFLV LDPSGEIVEK RADELEIGDY IVCTQKLVHE GMSEEELKRE VFRRLGRDFF 

       190        200        210        220        230        240 
VHLPEEEAES VLELAKERGI KALWETLEVD IEENSFYYQL RKGRIRADIL VDLAEELGLD 

       250        260        270        280        290        300 
LADLYDAVEV SYRSNTKSTK PIRLPEPEDL FYLAGLMFGD GCWNQLTNGS EAIQGEVKRI 

       310        320        330        340        350        360 
ASDMGLEVRV RRYEGKTARI DFPETVPRIL EALFDYPRRK KAHRIRVNDF LTRAPLDCIA 

       370        380        390        400        410        420 
EFIRGYFDAD GTVEEGRSAV SVTSVSREFL EDLQLLLQKF DVASYLREGD GAYTLYVSGA 

       430        440        450        460        470        480 
RSLERFPGFR EPEKAEKLKK LMEKASSSEL EKVPISGEIL REVRGDVPTT RMFNCYSNYE 

       490        500        510        520        530        540 
GGQVGLTKSS LEKVISTLEA VGVEGEALER LKALARDDVC FLEVVRVEEV EYDGYVYDFT 

       550        560        570        580        590        600 
VEEHHNFAAE GFVVHNTTLS DQLLAGAGMI SEELAGDQLV LDFDEMEQER GITIDAANVS 

       610        620        630        640        650        660 
MVHEYEGEEY LINLIDTPGH VDFSGDVTRA MRAVDGAIVV VCAVEGVMPQ TETVLRQALR 

       670        680        690        700        710        720 
ERVRPVLYIN KVDRLINELK LSPEEMQNRF LEIISEVNKM IEQMAPEEFK DEWKVSVEDG 

       730        740        750        760        770        780 
SVAFGSAYYG WGISFPFMEK TGITFKDIIE YCQQDKQKEL AQEAPVYQVV LDMVVKHLPD 

       790        800        810        820        830        840 
PVTAQEYRIE QIWPGDPESE DGKTLRKCDP NGKLAMVVTD VRIDEHAGEV ATGRVYSGTI 

       850        860        870        880        890        900 
REGQQVYLAS SKKETRVQQV GIYMGPDRIR TDEVPAGNIA AVTGLRDVWA GETVTDPEDP 

       910        920        930        940        950        960 
IEPFEELQHF AEPVVTVAVE AKNTQDLPKL IEILHQIAKE DPTVKVEINE ETGQHLVSGM 

       970        980        990       1000       1010       1020 
GELHLEIIAH RIKERGVDIK VSEPIVVYRE GVFGVCDDEV EGKSPNKHNK FYVTVEPVEE 

      1030       1040       1050       1060       1070       1080 
EIVEAIEEGK FNPEEMSKKE LEETLMEYGM DRDDAKAVET VKGTNFFLDK TVGLQYLNEV 

      1090       1100       1110       1120       1130       1140 
MELLIEGFEE AMEEGPLAKE PCRGVKVSLV DAEIHEDPVH RGPAQVIPAI KRAIYGGMLL 

      1150       1160       1170       1180       1190       1200 
ADTHLLEPMQ YIYVTVPQDY MGAVTKEIQG RRGTIEEIQQ EGDTVIIKGK APVAEMFGFA 

      1210       1220       1230       1240       1250 
NDIRSATEGR AIWTTEHAGY ERVPEELEEQ IIREIRERKG LKPEPPKPED YIEDYGG

« Hide

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01894.1.
RefSeq	NP_613964.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TXJ4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1476780.
GenomeReviews	Gene locus MK0679 in contig AE009439_GR.
KEGG	mka:MK0679.
NMPDR	fig\|190192.1.peg.677.
Phylogenomic databases
PhylomeDB	Q8TXJ4.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0679-MONOMER.
Family and domain databases
HAMAP	MF_00054_A. EF-G_EF-2_A. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004543. Transl_elong_EFG/EF2_arc. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K03234.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00490. AEF-2. 1 hit. TIGR01443. Intein_Cterm. 1 hit. TIGR01445. Intein_Nterm. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EF2_METKA

Accession

Primary (citable) accession number: Q8TXJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	June 1, 2002
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents