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Q8TXJ4 (EF2_METKA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor 2

Short name=EF-2

Cleaved into the following chain:

  1. Mka FusA intein
Gene names
Name:fusA
Ordered Locus Names:MK0679
OrganismMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]
Taxonomic identifier190192 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus

Protein attributes

Sequence length1257 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP-Rule MF_00054

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00054.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity. HAMAP-Rule MF_00054

Miscellaneous

The intein interrupts the GTP-binding site.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3434Elongation factor 2, 1st part Potential
PRO_0000007436
Chain35 – 556522Mka FusA intein Potential
PRO_0000007437
Chain557 – 1257701Elongation factor 2, 2nd part Potential
PRO_0000007438

Regions

Domain273 – 402130DOD-type homing endonuclease
Nucleotide binding616 – 6205GTP By similarity
Nucleotide binding670 – 6734GTP By similarity

Amino acid modifications

Modified residue11201Diphthamide By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TXJ4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 2C916583EF9D9B3C

FASTA1,257142,071
        10         20         30         40         50         60 
MGRKEKMAEK CRKLMTEPGK IRNIGIIAHI DHGKCVAPET KICLADGRFV RADELFEELK 

        70         80         90        100        110        120 
ERGRLVKCDE SEEVYELREP VGVSSLDKDA VEIVEGKITH VWRLKADKLV EVEVKNGRSI 

       130        140        150        160        170        180 
RTTPEHKFLV LDPSGEIVEK RADELEIGDY IVCTQKLVHE GMSEEELKRE VFRRLGRDFF 

       190        200        210        220        230        240 
VHLPEEEAES VLELAKERGI KALWETLEVD IEENSFYYQL RKGRIRADIL VDLAEELGLD 

       250        260        270        280        290        300 
LADLYDAVEV SYRSNTKSTK PIRLPEPEDL FYLAGLMFGD GCWNQLTNGS EAIQGEVKRI 

       310        320        330        340        350        360 
ASDMGLEVRV RRYEGKTARI DFPETVPRIL EALFDYPRRK KAHRIRVNDF LTRAPLDCIA 

       370        380        390        400        410        420 
EFIRGYFDAD GTVEEGRSAV SVTSVSREFL EDLQLLLQKF DVASYLREGD GAYTLYVSGA 

       430        440        450        460        470        480 
RSLERFPGFR EPEKAEKLKK LMEKASSSEL EKVPISGEIL REVRGDVPTT RMFNCYSNYE 

       490        500        510        520        530        540 
GGQVGLTKSS LEKVISTLEA VGVEGEALER LKALARDDVC FLEVVRVEEV EYDGYVYDFT 

       550        560        570        580        590        600 
VEEHHNFAAE GFVVHNTTLS DQLLAGAGMI SEELAGDQLV LDFDEMEQER GITIDAANVS 

       610        620        630        640        650        660 
MVHEYEGEEY LINLIDTPGH VDFSGDVTRA MRAVDGAIVV VCAVEGVMPQ TETVLRQALR 

       670        680        690        700        710        720 
ERVRPVLYIN KVDRLINELK LSPEEMQNRF LEIISEVNKM IEQMAPEEFK DEWKVSVEDG 

       730        740        750        760        770        780 
SVAFGSAYYG WGISFPFMEK TGITFKDIIE YCQQDKQKEL AQEAPVYQVV LDMVVKHLPD 

       790        800        810        820        830        840 
PVTAQEYRIE QIWPGDPESE DGKTLRKCDP NGKLAMVVTD VRIDEHAGEV ATGRVYSGTI 

       850        860        870        880        890        900 
REGQQVYLAS SKKETRVQQV GIYMGPDRIR TDEVPAGNIA AVTGLRDVWA GETVTDPEDP 

       910        920        930        940        950        960 
IEPFEELQHF AEPVVTVAVE AKNTQDLPKL IEILHQIAKE DPTVKVEINE ETGQHLVSGM 

       970        980        990       1000       1010       1020 
GELHLEIIAH RIKERGVDIK VSEPIVVYRE GVFGVCDDEV EGKSPNKHNK FYVTVEPVEE 

      1030       1040       1050       1060       1070       1080 
EIVEAIEEGK FNPEEMSKKE LEETLMEYGM DRDDAKAVET VKGTNFFLDK TVGLQYLNEV 

      1090       1100       1110       1120       1130       1140 
MELLIEGFEE AMEEGPLAKE PCRGVKVSLV DAEIHEDPVH RGPAQVIPAI KRAIYGGMLL 

      1150       1160       1170       1180       1190       1200 
ADTHLLEPMQ YIYVTVPQDY MGAVTKEIQG RRGTIEEIQQ EGDTVIIKGK APVAEMFGFA 

      1210       1220       1230       1240       1250 
NDIRSATEGR AIWTTEHAGY ERVPEELEEQ IIREIRERKG LKPEPPKPED YIEDYGG 

« Hide

References

[1]"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009439 Genomic DNA. Translation: AAM01894.1.
RefSeqNP_613964.1. NC_003551.1.

3D structure databases

ProteinModelPortalQ8TXJ4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190192.MK0679.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM01894; AAM01894; MK0679.
GeneID1476780.
KEGGmka:MK0679.

Phylogenomic databases

eggNOGCOG0480.
HOGENOMHOG000112334.
KOK03234.

Family and domain databases

Gene3D3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 3 hits.
HAMAPMF_00054_A. EF_G_EF_2_A.
InterProIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR00490. aEF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEF2_METKA
AccessionPrimary (citable) accession number: Q8TXJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries