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Q8TXJ4

- EF2_METKA

UniProt

Q8TXJ4 - EF2_METKA

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Protein
Elongation factor 2
Gene
fusA, MK0679
Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi616 – 6205GTP By similarity
Nucleotide bindingi670 – 6734GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. translation elongation factor activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor, Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Intron homing, Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Cleaved into the following chain:
Gene namesi
Name:fusA
Ordered Locus Names:MK0679
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3434Elongation factor 2, 1st part Reviewed prediction
PRO_0000007436Add
BLAST
Chaini35 – 556522Mka FusA intein Reviewed prediction
PRO_0000007437Add
BLAST
Chaini557 – 1257701Elongation factor 2, 2nd part Reviewed prediction
PRO_0000007438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1120 – 11201Diphthamide By similarity

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi190192.MK0679.

Structurei

3D structure databases

ProteinModelPortaliQ8TXJ4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini273 – 402130DOD-type homing endonuclease
Add
BLAST
Domaini541 – 782242tr-type G
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0480.
HOGENOMiHOG000112334.
KOiK03234.
OMAiHIDHGKC.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 3 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A.
InterProiIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TXJ4-1 [UniParc]FASTAAdd to Basket

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MGRKEKMAEK CRKLMTEPGK IRNIGIIAHI DHGKCVAPET KICLADGRFV     50
RADELFEELK ERGRLVKCDE SEEVYELREP VGVSSLDKDA VEIVEGKITH 100
VWRLKADKLV EVEVKNGRSI RTTPEHKFLV LDPSGEIVEK RADELEIGDY 150
IVCTQKLVHE GMSEEELKRE VFRRLGRDFF VHLPEEEAES VLELAKERGI 200
KALWETLEVD IEENSFYYQL RKGRIRADIL VDLAEELGLD LADLYDAVEV 250
SYRSNTKSTK PIRLPEPEDL FYLAGLMFGD GCWNQLTNGS EAIQGEVKRI 300
ASDMGLEVRV RRYEGKTARI DFPETVPRIL EALFDYPRRK KAHRIRVNDF 350
LTRAPLDCIA EFIRGYFDAD GTVEEGRSAV SVTSVSREFL EDLQLLLQKF 400
DVASYLREGD GAYTLYVSGA RSLERFPGFR EPEKAEKLKK LMEKASSSEL 450
EKVPISGEIL REVRGDVPTT RMFNCYSNYE GGQVGLTKSS LEKVISTLEA 500
VGVEGEALER LKALARDDVC FLEVVRVEEV EYDGYVYDFT VEEHHNFAAE 550
GFVVHNTTLS DQLLAGAGMI SEELAGDQLV LDFDEMEQER GITIDAANVS 600
MVHEYEGEEY LINLIDTPGH VDFSGDVTRA MRAVDGAIVV VCAVEGVMPQ 650
TETVLRQALR ERVRPVLYIN KVDRLINELK LSPEEMQNRF LEIISEVNKM 700
IEQMAPEEFK DEWKVSVEDG SVAFGSAYYG WGISFPFMEK TGITFKDIIE 750
YCQQDKQKEL AQEAPVYQVV LDMVVKHLPD PVTAQEYRIE QIWPGDPESE 800
DGKTLRKCDP NGKLAMVVTD VRIDEHAGEV ATGRVYSGTI REGQQVYLAS 850
SKKETRVQQV GIYMGPDRIR TDEVPAGNIA AVTGLRDVWA GETVTDPEDP 900
IEPFEELQHF AEPVVTVAVE AKNTQDLPKL IEILHQIAKE DPTVKVEINE 950
ETGQHLVSGM GELHLEIIAH RIKERGVDIK VSEPIVVYRE GVFGVCDDEV 1000
EGKSPNKHNK FYVTVEPVEE EIVEAIEEGK FNPEEMSKKE LEETLMEYGM 1050
DRDDAKAVET VKGTNFFLDK TVGLQYLNEV MELLIEGFEE AMEEGPLAKE 1100
PCRGVKVSLV DAEIHEDPVH RGPAQVIPAI KRAIYGGMLL ADTHLLEPMQ 1150
YIYVTVPQDY MGAVTKEIQG RRGTIEEIQQ EGDTVIIKGK APVAEMFGFA 1200
NDIRSATEGR AIWTTEHAGY ERVPEELEEQ IIREIRERKG LKPEPPKPED 1250
YIEDYGG 1257
Length:1,257
Mass (Da):142,071
Last modified:June 1, 2002 - v1
Checksum:i2C916583EF9D9B3C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM01894.1.
RefSeqiNP_613964.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM01894; AAM01894; MK0679.
GeneIDi1476780.
KEGGimka:MK0679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM01894.1 .
RefSeqi NP_613964.1. NC_003551.1.

3D structure databases

ProteinModelPortali Q8TXJ4.
ModBasei Search...

Protein-protein interaction databases

STRINGi 190192.MK0679.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM01894 ; AAM01894 ; MK0679 .
GeneIDi 1476780.
KEGGi mka:MK0679.

Phylogenomic databases

eggNOGi COG0480.
HOGENOMi HOG000112334.
KOi K03234.
OMAi HIDHGKC.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 3 hits.
HAMAPi MF_00054_A. EF_G_EF_2_A.
InterProi IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR00490. aEF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiEF2_METKA
AccessioniPrimary (citable) accession number: Q8TXJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the GTP-binding site.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi