Q8TXJ4 (EF2_METKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor 2
Short name=EF-2

Cleaved into the following chain:

1. Mka FusA intein

Gene names

Name:	fusA
Ordered Locus Names:	MK0679

Organism

Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) [Reference proteome] [HAMAP]

Taxonomic identifier

190192 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus › Methanopyrus kandleri

Protein attributes

Sequence length	1257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP-Rule MF_00054
Subcellular location	Cytoplasm By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity. HAMAP-Rule MF_00054
Miscellaneous	The intein interrupts the GTP-binding site. HAMAP-Rule MF_00054
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 34	34	Elongation factor 2, 1st part Potential		PRO_0000007436
	Chain	35 – 556	522	Mka FusA intein Potential		PRO_0000007437
	Chain	557 – 1257	701	Elongation factor 2, 2nd part Potential		PRO_0000007438
Regions
	Domain	273 – 402	130	DOD-type homing endonuclease
	Nucleotide binding	616 – 620	5	GTP By similarity
	Nucleotide binding	670 – 673	4	GTP By similarity
Amino acid modifications
	Modified residue	1120	1	Diphthamide By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TXJ4 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2C916583EF9D9B3C	FASTA	1,257	142,071
	10 20 30 40 50 60 MGRKEKMAEK CRKLMTEPGK IRNIGIIAHI DHGKCVAPET KICLADGRFV RADELFEELK 70 80 90 100 110 120 ERGRLVKCDE SEEVYELREP VGVSSLDKDA VEIVEGKITH VWRLKADKLV EVEVKNGRSI 130 140 150 160 170 180 RTTPEHKFLV LDPSGEIVEK RADELEIGDY IVCTQKLVHE GMSEEELKRE VFRRLGRDFF 190 200 210 220 230 240 VHLPEEEAES VLELAKERGI KALWETLEVD IEENSFYYQL RKGRIRADIL VDLAEELGLD 250 260 270 280 290 300 LADLYDAVEV SYRSNTKSTK PIRLPEPEDL FYLAGLMFGD GCWNQLTNGS EAIQGEVKRI 310 320 330 340 350 360 ASDMGLEVRV RRYEGKTARI DFPETVPRIL EALFDYPRRK KAHRIRVNDF LTRAPLDCIA 370 380 390 400 410 420 EFIRGYFDAD GTVEEGRSAV SVTSVSREFL EDLQLLLQKF DVASYLREGD GAYTLYVSGA 430 440 450 460 470 480 RSLERFPGFR EPEKAEKLKK LMEKASSSEL EKVPISGEIL REVRGDVPTT RMFNCYSNYE 490 500 510 520 530 540 GGQVGLTKSS LEKVISTLEA VGVEGEALER LKALARDDVC FLEVVRVEEV EYDGYVYDFT 550 560 570 580 590 600 VEEHHNFAAE GFVVHNTTLS DQLLAGAGMI SEELAGDQLV LDFDEMEQER GITIDAANVS 610 620 630 640 650 660 MVHEYEGEEY LINLIDTPGH VDFSGDVTRA MRAVDGAIVV VCAVEGVMPQ TETVLRQALR 670 680 690 700 710 720 ERVRPVLYIN KVDRLINELK LSPEEMQNRF LEIISEVNKM IEQMAPEEFK DEWKVSVEDG 730 740 750 760 770 780 SVAFGSAYYG WGISFPFMEK TGITFKDIIE YCQQDKQKEL AQEAPVYQVV LDMVVKHLPD 790 800 810 820 830 840 PVTAQEYRIE QIWPGDPESE DGKTLRKCDP NGKLAMVVTD VRIDEHAGEV ATGRVYSGTI 850 860 870 880 890 900 REGQQVYLAS SKKETRVQQV GIYMGPDRIR TDEVPAGNIA AVTGLRDVWA GETVTDPEDP 910 920 930 940 950 960 IEPFEELQHF AEPVVTVAVE AKNTQDLPKL IEILHQIAKE DPTVKVEINE ETGQHLVSGM 970 980 990 1000 1010 1020 GELHLEIIAH RIKERGVDIK VSEPIVVYRE GVFGVCDDEV EGKSPNKHNK FYVTVEPVEE 1030 1040 1050 1060 1070 1080 EIVEAIEEGK FNPEEMSKKE LEETLMEYGM DRDDAKAVET VKGTNFFLDK TVGLQYLNEV 1090 1100 1110 1120 1130 1140 MELLIEGFEE AMEEGPLAKE PCRGVKVSLV DAEIHEDPVH RGPAQVIPAI KRAIYGGMLL 1150 1160 1170 1180 1190 1200 ADTHLLEPMQ YIYVTVPQDY MGAVTKEIQG RRGTIEEIQQ EGDTVIIKGK APVAEMFGFA 1210 1220 1230 1240 1250 NDIRSATEGR AIWTTEHAGY ERVPEELEEQ IIREIRERKG LKPEPPKPED YIEDYGG

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009439 Genomic DNA. Translation: AAM01894.1.
RefSeq	NP_613964.1. NC_003551.1.
3D structure databases
ProteinModelPortal	Q8TXJ4.
ModBase	Search...
Protein-protein interaction databases
STRING	190192.MK0679.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM01894; AAM01894; MK0679.
GeneID	1476780.
KEGG	mka:MK0679.
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HOG000112334.
KO	K03234.
Family and domain databases
Gene3D	3.30.230.10. 1 hit. 3.30.70.240. 1 hit.
HAMAP	MF_00054_A. EF-G_EF-2_A.
InterPro	IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004543. Transl_elong_EFG/EF2_arc. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00490. aEF-2. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EF2_METKA

Accession

Primary (citable) accession number: Q8TXJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program