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Q8TXJ4

- EF2_METKA

UniProt

Q8TXJ4 - EF2_METKA

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Protein

Elongation factor 2

Gene

fusA

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi616 – 6205GTPUniRule annotation
Nucleotide bindingi670 – 6734GTPUniRule annotation

GO - Molecular functioni

  1. endonuclease activity Source: UniProtKB-KW
  2. GTPase activity Source: InterPro
  3. GTP binding Source: UniProtKB-KW
  4. translation elongation factor activity Source: UniProtKB-KW

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor, Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Intron homing, Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2UniRule annotation
Short name:
EF-2UniRule annotation
Cleaved into the following chain:
Gene namesi
Name:fusAUniRule annotation
Ordered Locus Names:MK0679
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3434Elongation factor 2, 1st partSequence AnalysisPRO_0000007436Add
BLAST
Chaini35 – 556522Mka FusA inteinSequence AnalysisPRO_0000007437Add
BLAST
Chaini557 – 1257701Elongation factor 2, 2nd partSequence AnalysisPRO_0000007438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1120 – 11201DiphthamideUniRule annotation

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.By similarity

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi190192.MK0679.

Structurei

3D structure databases

ProteinModelPortaliQ8TXJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini273 – 402130DOD-type homing endonucleaseAdd
BLAST
Domaini541 – 782242tr-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0480.
HOGENOMiHOG000112334.
KOiK03234.
OMAiHIDHGKC.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 3 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A.
InterProiIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TXJ4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRKEKMAEK CRKLMTEPGK IRNIGIIAHI DHGKCVAPET KICLADGRFV
60 70 80 90 100
RADELFEELK ERGRLVKCDE SEEVYELREP VGVSSLDKDA VEIVEGKITH
110 120 130 140 150
VWRLKADKLV EVEVKNGRSI RTTPEHKFLV LDPSGEIVEK RADELEIGDY
160 170 180 190 200
IVCTQKLVHE GMSEEELKRE VFRRLGRDFF VHLPEEEAES VLELAKERGI
210 220 230 240 250
KALWETLEVD IEENSFYYQL RKGRIRADIL VDLAEELGLD LADLYDAVEV
260 270 280 290 300
SYRSNTKSTK PIRLPEPEDL FYLAGLMFGD GCWNQLTNGS EAIQGEVKRI
310 320 330 340 350
ASDMGLEVRV RRYEGKTARI DFPETVPRIL EALFDYPRRK KAHRIRVNDF
360 370 380 390 400
LTRAPLDCIA EFIRGYFDAD GTVEEGRSAV SVTSVSREFL EDLQLLLQKF
410 420 430 440 450
DVASYLREGD GAYTLYVSGA RSLERFPGFR EPEKAEKLKK LMEKASSSEL
460 470 480 490 500
EKVPISGEIL REVRGDVPTT RMFNCYSNYE GGQVGLTKSS LEKVISTLEA
510 520 530 540 550
VGVEGEALER LKALARDDVC FLEVVRVEEV EYDGYVYDFT VEEHHNFAAE
560 570 580 590 600
GFVVHNTTLS DQLLAGAGMI SEELAGDQLV LDFDEMEQER GITIDAANVS
610 620 630 640 650
MVHEYEGEEY LINLIDTPGH VDFSGDVTRA MRAVDGAIVV VCAVEGVMPQ
660 670 680 690 700
TETVLRQALR ERVRPVLYIN KVDRLINELK LSPEEMQNRF LEIISEVNKM
710 720 730 740 750
IEQMAPEEFK DEWKVSVEDG SVAFGSAYYG WGISFPFMEK TGITFKDIIE
760 770 780 790 800
YCQQDKQKEL AQEAPVYQVV LDMVVKHLPD PVTAQEYRIE QIWPGDPESE
810 820 830 840 850
DGKTLRKCDP NGKLAMVVTD VRIDEHAGEV ATGRVYSGTI REGQQVYLAS
860 870 880 890 900
SKKETRVQQV GIYMGPDRIR TDEVPAGNIA AVTGLRDVWA GETVTDPEDP
910 920 930 940 950
IEPFEELQHF AEPVVTVAVE AKNTQDLPKL IEILHQIAKE DPTVKVEINE
960 970 980 990 1000
ETGQHLVSGM GELHLEIIAH RIKERGVDIK VSEPIVVYRE GVFGVCDDEV
1010 1020 1030 1040 1050
EGKSPNKHNK FYVTVEPVEE EIVEAIEEGK FNPEEMSKKE LEETLMEYGM
1060 1070 1080 1090 1100
DRDDAKAVET VKGTNFFLDK TVGLQYLNEV MELLIEGFEE AMEEGPLAKE
1110 1120 1130 1140 1150
PCRGVKVSLV DAEIHEDPVH RGPAQVIPAI KRAIYGGMLL ADTHLLEPMQ
1160 1170 1180 1190 1200
YIYVTVPQDY MGAVTKEIQG RRGTIEEIQQ EGDTVIIKGK APVAEMFGFA
1210 1220 1230 1240 1250
NDIRSATEGR AIWTTEHAGY ERVPEELEEQ IIREIRERKG LKPEPPKPED

YIEDYGG
Length:1,257
Mass (Da):142,071
Last modified:June 1, 2002 - v1
Checksum:i2C916583EF9D9B3C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM01894.1.
RefSeqiNP_613964.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM01894; AAM01894; MK0679.
GeneIDi1476780.
KEGGimka:MK0679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009439 Genomic DNA. Translation: AAM01894.1 .
RefSeqi NP_613964.1. NC_003551.1.

3D structure databases

ProteinModelPortali Q8TXJ4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190192.MK0679.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM01894 ; AAM01894 ; MK0679 .
GeneIDi 1476780.
KEGGi mka:MK0679.

Phylogenomic databases

eggNOGi COG0480.
HOGENOMi HOG000112334.
KOi K03234.
OMAi HIDHGKC.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 3 hits.
HAMAPi MF_00054_A. EF_G_EF_2_A.
InterProi IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR00490. aEF-2. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiEF2_METKA
AccessioniPrimary (citable) accession number: Q8TXJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the GTP-binding site.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3