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Reviewed, UniProtKB/Swiss-Prot Q8TXF7 (ACDA1_METKA)

Last modified June 16, 2009. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Acetyl-CoA decarbonylase/synthase complex subunit alpha 1
      Short name=ACDS complex subunit alpha 1
    EC=1.2.99.2
Alternative name(s):
    ACDS complex carbon monoxide dehydrogenase 1
      Short name=ACDS CODH 1
Gene names
Name: cdhA1
Ordered Locus Names: MK0717
OrganismMethanopyrus kandleri [Complete proteome] [HAMAP]
Taxonomic identifier2320 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

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Protein attributes

Sequence length760 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO2 By similarity.

Catalytic activity

CO + H2O + A = CO2 + AH2. HAMAP MF_01137

Cofactor

Binds 7 4Fe-4S clusters per heterotetramer Potential.

Binds 2 nickel-iron-sulfur clusters per heterotetramer Potential.

Subunit structure

Heterotetramer of two alpha and two epsilon chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha2epsilon2)(4)-beta(8)-(gamma1delta1)8 Potential.

Domain

Cluster B is an all-cysteinyl-liganded 4Fe4S cluster; cluster C is a mixed Ni-Fe-S cluster which appears to be the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe4S cluster that bridges the two subunits of the CODH dimer. May contain two additional 4Fe-4S clusters, dubbed E and F, that might reroute electron transfer along different paths. HAMAP MF_01137

Sequence similarities

Belongs to the Ni-containing carbon monoxide dehydrogenase family.

Contains 2 4Fe-4S ferredoxin-type domains.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 760760Acetyl-CoA decarbonylase/synthase complex subunit alpha 1 HAMAP MF_01137
PRO_0000155078

Regions

Domain381 – 410304Fe-4S ferredoxin-type 1
Domain418 – 450334Fe-4S ferredoxin-type 2

Sites

Metal binding561Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity
Metal binding591Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity
Metal binding601Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding621Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding671Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding771Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2311Nickel-iron-sulfur By similarity
Metal binding2591Nickel-iron-sulfur By similarity
Metal binding2981Nickel-iron-sulfur By similarity
Metal binding3901Iron-sulfur 3 (4Fe-4S) Potential
Metal binding3931Iron-sulfur 3 (4Fe-4S) Potential
Metal binding3961Iron-sulfur 3 (4Fe-4S) Potential
Metal binding4001Iron-sulfur 3 (4Fe-4S) Potential
Metal binding4281Iron-sulfur 4 (4Fe-4S) Potential
Metal binding4311Iron-sulfur 4 (4Fe-4S) Potential
Metal binding4341Iron-sulfur 4 (4Fe-4S) Potential
Metal binding4381Iron-sulfur 4 (4Fe-4S) Potential
Metal binding4961Nickel-iron-sulfur By similarity
Metal binding5251Nickel-iron-sulfur By similarity
Metal binding5601Nickel-iron-sulfur By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q8TXF7-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: E82B0EF7D4163CAA

FASTA76084,637
        10         20         30         40         50         60 
MSPFELEFEG LKVQIGSIEG FEPRGEGPLP CPTVSDLADW DRKLFARYRV IAFPICDMCC 

        70         80         90        100        110        120 
MCTYGRCNLA EGRRGACGID IRSNTARFTA LKTCIGAACH AAHARHLVEY ILEKLGDVEI 

       130        140        150        160        170        180 
DLGSEVDVMT PIFETLVGFK PKTVSDLEKG LEYIERELTK VLSSVHVGQE MDPHDYESKA 

       190        200        210        220        230        240 
LHAGMIDNLA LEIADVAQIA AFDMPKGEAP LVEFGPFAAD DSKPCILLVG HNVAPGTEVL 

       250        260        270        280        290        300 
DYLEERGLDE EVEVLGICCT AWDVSRVDDR SKVIGPLSRQ LHYVRMGIAD VVVLDEQCIR 

       310        320        330        340        350        360 
ADIVEEANEV GSRVIATRDL VMAGLPDVTD EPTEKIIEKM VSGEWMGVFI EDLEKAAEVA 

       370        380        390        400        410        420 
VEVAIRVHER RKKEIPQPDP KKLQKEAKRC LGCGDCERVC PNDLPIVEAM ERAANGDFEG 

       430        440        450        460        470        480 
LADLFDRCVG CARCESECPT KLRVMNMIED AWRLRTKEEK YKVRTGRGPI KDVEIRQVGG 

       490        500        510        520        530        540 
PIVMGDIPGV VAFVACPNYP DDVKQVGKMV EELLERNYIV LTSGCTAMAL GMYTDEDGKT 

       550        560        570        580        590        600 
LYEKYEDRFD AGCLVNTGSC VSNAHILGAC IKIAAIFAKK PLKGNFKEIA DYILNRIGAC 

       610        620        630        640        650        660 
GVLWGTMSQK ALAISTGFTR WGIPIVYGPA GLKYQTLYIG DLDGDWTVYD ARTGKECKEY 

       670        680        690        700        710        720 
CPIHLKYAAE DWREALVQAV KLCIRPNDTP QGRQTKLQNY IELYKEFYNE LPPDLPLYVR 

       730        740        750        760 
DKNDVPITLR DEVMEYLEEV GWKPRKGITE PTLLEENVRG

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References

[1]"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens."
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

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Cross-references

Sequence databases

AE010365 Genomic DNA. Translation: AAM01931.1.
RefSeqNP_614001.1.

3D structure databases

HSSPHSSP built from PDB template 1G6B based on UniProtKB P00214.
ModBaseSearch...

Genome annotation databases

GeneID1476818.
GenomeReviewsGene locus MK0717 in contig AE009439_GR.
KEGGmka:MK0717.
NMPDRfig|190192.1.peg.714.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ8TXF7.
OMAQ8TXF7. ICCTAID.

Enzyme and pathway databases

BioCycMKAN190192:MK0717-MON.
BRENDA1.2.99.2. 7577.

Family and domain databases

HAMAPMF_01137.
[Tree]
InterProIPR017896. 4Fe4S_Fe-S-bd.
IPR001450. 4Fe4S_Fe_S_bd_subgr.
IPR017900. 4Fe4S_Fe_S_CS.
IPR004460. CO_DH/Ac-CoA_synth_asu.
IPR016101. CO_DH_a-bundle.
IPR004137. Prismane.
IPR016099. Prismane-like_a/b-sand.
[Graphical view]
Gene3DG3DSA:1.20.1270.30. CO_DH_a-bundle. 1 hit.
G3DSA:3.40.50.2030. Prismane-like_a/b-sand. 2 hits.
PfamPF00037. Fer4. 2 hits.
PF03063. Prismane. 1 hit.
[Graphical view]
TIGRFAMsTIGR00314. cdhA. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameACDA1_METKA
AccessionPrimary (citable) accession number: Q8TXF7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: June 1, 2002
Last modified: June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents