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Protein

FO synthase subunit 2

Gene

cofH

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO).UniRule annotation

Catalytic activityi

5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H2O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH3.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: coenzyme F0 biosynthesis

This protein is involved in the pathway coenzyme F0 biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway coenzyme F0 biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi74 – 741Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi77 – 771Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00072.

Names & Taxonomyi

Protein namesi
Recommended name:
FO synthase subunit 2UniRule annotation (EC:2.5.1.77UniRule annotation)
Alternative name(s):
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2UniRule annotation
Gene namesi
Name:cofHUniRule annotation
Ordered Locus Names:MK0897
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369FO synthase subunit 2PRO_0000141719Add
BLAST

Interactioni

Subunit structurei

Consists of two subunits, CofG and CofH.UniRule annotation

Protein-protein interaction databases

STRINGi190192.MK0897.

Structurei

3D structure databases

ProteinModelPortaliQ8TWY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. CofH family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00656. Archaea.
COG1060. LUCA.
HOGENOMiHOG000224281.
KOiK11781.
OMAiYGHVENE.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01612. FO_synth_sub2.
InterProiIPR013785. Aldolase_TIM.
IPR019940. CofH_family.
IPR006638. Elp3/MiaB/NifB.
IPR020050. FO_synthase_su2.
IPR005244. FO_synthase_su2-like.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF004762. CHP00423. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03551. F420_cofH. 1 hit.
TIGR00423. TIGR00423. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TWY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDSPDEVSV DELVNMAVAG GIDEGTALDA LQGKLDPYKV MRAAHEARLK
60 70 80 90 100
IVGEHVTFVV NRNINFTNVC INRCRFCAFR RDPDDPDAYR MTPEEVGERA
110 120 130 140 150
AEARDAGATE VCLQGGLHPE ATFEYYLEML DEIKSQAPDI HVHGYSPMEV
160 170 180 190 200
KYCAKLAGED IEDVLRELKR AGLDSMPGTA AEIFSPEVRK RLCPDKLEAD
210 220 230 240 250
EWEHIIRIAH ELGIPTTCTM MYGHIDSPRD WIDHMKRLRG IQEDTGGFTE
260 270 280 290 300
FVPLSFVHSN APIYRRGGAR PGVSGMTDVL VHAVARLYFG PLIPNIQASW
310 320 330 340 350
VKLGVKLAQM TLHAGANDLG GTLMEENISR EAGATEGEQL EPEEIVEIIR
360
EAGFTPVQRT TLYEPVKVY
Length:369
Mass (Da):41,064
Last modified:June 1, 2002 - v1
Checksum:i3BC771AA5C211AFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM02110.1.

Genome annotation databases

EnsemblBacteriaiAAM02110; AAM02110; MK0897.
KEGGimka:MK0897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM02110.1.

3D structure databases

ProteinModelPortaliQ8TWY4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190192.MK0897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM02110; AAM02110; MK0897.
KEGGimka:MK0897.

Phylogenomic databases

eggNOGiarCOG00656. Archaea.
COG1060. LUCA.
HOGENOMiHOG000224281.
KOiK11781.
OMAiYGHVENE.

Enzyme and pathway databases

UniPathwayiUPA00072.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01612. FO_synth_sub2.
InterProiIPR013785. Aldolase_TIM.
IPR019940. CofH_family.
IPR006638. Elp3/MiaB/NifB.
IPR020050. FO_synthase_su2.
IPR005244. FO_synthase_su2-like.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF004762. CHP00423. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03551. F420_cofH. 1 hit.
TIGR00423. TIGR00423. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiCOFH_METKA
AccessioniPrimary (citable) accession number: Q8TWY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.