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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei631 – 6311ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MK0899
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
ProteomesiUP000001826: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10801080Isoleucine--tRNA ligasePRO_0000098582Add
BLAST

Proteomic databases

PRIDEiQ8TWY2.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi190192.MK0899.

Structurei

3D structure databases

ProteinModelPortaliQ8TWY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi628 – 6325"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiVLGDWDN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TWY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAGEMAEAL GEELEREIQR RWEEMDLLSK VLEKNRDGPL FYFLDGPPYA
60 70 80 90 100
SGSIHLGTAW NKIIKDAVNR YKLMRGYRVR LQPGWDCHGL PIEVKVEQEV
110 120 130 140 150
LSDEIECKKD IEEKVGVDKF VEKCKEFALK HVEIMTEQFK RLGVLMDWDN
160 170 180 190 200
PYMTLDNEYI EGAWYTLKRA HERGLLDRDV RIVNWCPRCE TALADHEVEY
210 220 230 240 250
KEVEDPSIFV IFPIEDDSDA EVDLPENSAL LIWTTTPWTL PANLAVAVHP
260 270 280 290 300
EEEYVLARAE VDGEEWHLIV ADKLKVVLSV VTDSYEIVDS FPGEALEGLR
310 320 330 340 350
YRPPLWEEVP KLRELHEEDD RVHRVYTAEW VTMEEGTGCV HSAPGHGEED
360 370 380 390 400
FELGREVGLP PHCPVAEDGT FTEDGGKYEG LYVRDANEKI VEDLREKGLL
410 420 430 440 450
AHEDTVEHRY GHCWRCKTPI IYRATEQWFL KVTEVKDEML EWIERVEWIP
460 470 480 490 500
EWAGHSRFKS WVENARDWCI SRQRYWGIPL PVWECEECGH LEVIGSLSEL
510 520 530 540 550
EAKAVSLPPG EPDLHRPWVD EVVLKCPECG SYMRRVPDVL DVWVDSGVAA
560 570 580 590 600
WASLGYPRRE DEFERWFLKE GRCDPDDPEA GADFITEGHD QTRGWFYSQL
610 620 630 640 650
GCGVVTFDTC PYRTVLMHGF TLDEEGRKMS KSLGNVVDPM DVVEKYGADT
660 670 680 690 700
LRWYVLRSNA PWRDMHFSWQ DVRDTHRALN VLWNAYRFTK MYSELDEFDP
710 720 730 740 750
EEHPLEDLEE HLKPEDRWLL SRINSLVEEV TDAFERYHVH EAARALYRFV
760 770 780 790 800
TEDLSRWYIR LVRERVWLEG DDPEKLAVYA VLHYTFDRLV RLLAPIVPHV
810 820 830 840 850
AERIYLDYVR AGDDPESVHL TDWPEVDDRW VDEGLEKAME LVRKAAEAAL
860 870 880 890 900
SVRQRAGVKT RWPLRRLFVE VEDPKRLEDL KDVLARVANV KEVELGEEFP
910 920 930 940 950
EKVPVAEPRP DKIGPEFRSL AGRVIEHVKD RAEEVARSIL KDGEYRTELD
960 970 980 990 1000
GEDVVLTEEH VKVTEDLPEG WEAEEFEGGR VYVFVELDEE LKSEAWAREV
1010 1020 1030 1040 1050
VRRVQEMRKE LDLNLEERIR VWIETDEEIA EAVEEHSEYV RGETRADELH
1060 1070 1080
VNEGWPEEVD LEREWEVEDR TIRIAVVVSG
Length:1,080
Mass (Da):126,028
Last modified:June 1, 2002 - v1
Checksum:i0024CBCD26D75BC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM02112.1.
RefSeqiNP_614182.1. NC_003551.1.

Genome annotation databases

EnsemblBacteriaiAAM02112; AAM02112; MK0899.
GeneIDi1477000.
KEGGimka:MK0899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA. Translation: AAM02112.1.
RefSeqiNP_614182.1. NC_003551.1.

3D structure databases

ProteinModelPortaliQ8TWY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190192.MK0899.

Proteomic databases

PRIDEiQ8TWY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM02112; AAM02112; MK0899.
GeneIDi1477000.
KEGGimka:MK0899.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiVLGDWDN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Entry informationi

Entry nameiSYI_METKA
AccessioniPrimary (citable) accession number: Q8TWY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: March 4, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.