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Q8TWY2

- SYI_METKA

UniProt

Q8TWY2 - SYI_METKA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei631 – 6311ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MK0899
    OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
    Taxonomic identifieri190192 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
    ProteomesiUP000001826: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10801080Isoleucine--tRNA ligasePRO_0000098582Add
    BLAST

    Proteomic databases

    PRIDEiQ8TWY2.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi190192.MK0899.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TWY2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi628 – 6325"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiADNINIY.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8TWY2-1 [UniParc]FASTAAdd to Basket

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    MGAGEMAEAL GEELEREIQR RWEEMDLLSK VLEKNRDGPL FYFLDGPPYA     50
    SGSIHLGTAW NKIIKDAVNR YKLMRGYRVR LQPGWDCHGL PIEVKVEQEV 100
    LSDEIECKKD IEEKVGVDKF VEKCKEFALK HVEIMTEQFK RLGVLMDWDN 150
    PYMTLDNEYI EGAWYTLKRA HERGLLDRDV RIVNWCPRCE TALADHEVEY 200
    KEVEDPSIFV IFPIEDDSDA EVDLPENSAL LIWTTTPWTL PANLAVAVHP 250
    EEEYVLARAE VDGEEWHLIV ADKLKVVLSV VTDSYEIVDS FPGEALEGLR 300
    YRPPLWEEVP KLRELHEEDD RVHRVYTAEW VTMEEGTGCV HSAPGHGEED 350
    FELGREVGLP PHCPVAEDGT FTEDGGKYEG LYVRDANEKI VEDLREKGLL 400
    AHEDTVEHRY GHCWRCKTPI IYRATEQWFL KVTEVKDEML EWIERVEWIP 450
    EWAGHSRFKS WVENARDWCI SRQRYWGIPL PVWECEECGH LEVIGSLSEL 500
    EAKAVSLPPG EPDLHRPWVD EVVLKCPECG SYMRRVPDVL DVWVDSGVAA 550
    WASLGYPRRE DEFERWFLKE GRCDPDDPEA GADFITEGHD QTRGWFYSQL 600
    GCGVVTFDTC PYRTVLMHGF TLDEEGRKMS KSLGNVVDPM DVVEKYGADT 650
    LRWYVLRSNA PWRDMHFSWQ DVRDTHRALN VLWNAYRFTK MYSELDEFDP 700
    EEHPLEDLEE HLKPEDRWLL SRINSLVEEV TDAFERYHVH EAARALYRFV 750
    TEDLSRWYIR LVRERVWLEG DDPEKLAVYA VLHYTFDRLV RLLAPIVPHV 800
    AERIYLDYVR AGDDPESVHL TDWPEVDDRW VDEGLEKAME LVRKAAEAAL 850
    SVRQRAGVKT RWPLRRLFVE VEDPKRLEDL KDVLARVANV KEVELGEEFP 900
    EKVPVAEPRP DKIGPEFRSL AGRVIEHVKD RAEEVARSIL KDGEYRTELD 950
    GEDVVLTEEH VKVTEDLPEG WEAEEFEGGR VYVFVELDEE LKSEAWAREV 1000
    VRRVQEMRKE LDLNLEERIR VWIETDEEIA EAVEEHSEYV RGETRADELH 1050
    VNEGWPEEVD LEREWEVEDR TIRIAVVVSG 1080
    Length:1,080
    Mass (Da):126,028
    Last modified:June 1, 2002 - v1
    Checksum:i0024CBCD26D75BC0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009439 Genomic DNA. Translation: AAM02112.1.
    RefSeqiNP_614182.1. NC_003551.1.
    WP_011019267.1. NC_003551.1.

    Genome annotation databases

    EnsemblBacteriaiAAM02112; AAM02112; MK0899.
    GeneIDi1477000.
    KEGGimka:MK0899.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009439 Genomic DNA. Translation: AAM02112.1 .
    RefSeqi NP_614182.1. NC_003551.1.
    WP_011019267.1. NC_003551.1.

    3D structure databases

    ProteinModelPortali Q8TWY2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 190192.MK0899.

    Proteomic databases

    PRIDEi Q8TWY2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM02112 ; AAM02112 ; MK0899 .
    GeneIDi 1477000.
    KEGGi mka:MK0899.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ADNINIY.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

    Entry informationi

    Entry nameiSYI_METKA
    AccessioniPrimary (citable) accession number: Q8TWY2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3